Seminal Fluid Flashcards
Most common male infertility is
Varicocele
Hardening pf veins that drain the testes
Varicocele
_______ samples be collected not less than 7 days or more than 3 weeks apart
2-3 samples
____ samples is considered significant in fertility testing
2 abnormal samples
Reasons for analysis of semen
✓ fertility testing
✓ post- vasectomy analysis
✓ forensic analysis
Collection of semen
Atleast 2 days to not more than 7 days
Spx awaiting analysis should be kept at
37 celsius
Reasons why spx should not exceed more than 7 days
Prolonged abstinence will result to:
✓Higher volume
✓Decreased sperm motility
✓Increased flavin giving the semen a yellowish color
What will happen if the first portion of ejaculate is missing
✓decrease sperm count
✓increase pH
✓ spx will not liquefy
What will happen if the last portion of ejaculate is missing
✓ increased sperm count
✓ decreased pH
✓ spx will not clot
✓ decrease semen volume
Fresh seme spz is clotted and should be liquefy within
30-60 mins after collection
Failure to liquefy may be caused by
Deficiency in prostatic enzyme
What should be adder if the semen spx is not yes liqeufy after 2hrs
Dulbecco’s phosphate-buffered saline or proteolytic enzyme
Stage of cellular maturation
Spermatogonia - spermatocytes - spermatids - spermatozoa
Seminal fluid contains high concentration of
Prostatic acid phosphatase
Test used for suspected rape case
Medico legal
To assess sperm morphology used
Papanicolaou’s
Wright stain
Giemsa
To assess sperm viability used
Eosin Nigrosin
To assess sperm count
Dilute with cjilled tap water or formalin bicarbonate solution
How many days is sperm maturation
90 days
Normal volume of semen
2-5 mL
Normal appearance of semen
Gray white translucent
Incompletely liquefied spx are
Clumped and highly viscous
Semen is considered highly viscous when the threads are ______
longer than 2cm
Rating of semen viscosity
0 (watery) to 4 (gel-like)
Normal viscosity
Pour in droplets and will not appear clumped and stingy
Normal pH of semen
7.2 to 8.0
Reference value for sperm concentration
20M to 250 M sperm/ mL
Borderline of sperm concentration
10M to 20M/ mL
Normal sperm count
> 40M per ejaculate
How to evaluate motility
Speed and direction
Normal sper motility
50% with a rating of 2.0 after 1 hr
Provides objective determination of both sperm velocity and trajectory
Computer assisted semen analysis (casa)
Routine Criteria for semen analysis
✓ thin smears
✓ stains using giemsa, shorr, or papanicolaou’s
✓ atleast 200 sperms should be evaluated
