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CSF Flashcards

1
Q

Third major body fluid

A

CSF

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2
Q

Who recognized CSF

A

Cotugno ( 1764)

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3
Q

It lines the brain and spinal cord

A

Meninges

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4
Q

T or F:
CSF is NOT an ultrafiltrate of plasma

A

True

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5
Q

3 layers of meninges

A

Dura mater– outer
Arachnoid space
Pia mater— inner

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6
Q

Latin word fo dura mater

A

Hard mother

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7
Q

Latin word for pia mater

A

Gentle mother

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8
Q

Spiderweb like layer

A

Arachnoid layer

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9
Q

It is where CSF flows

A

Subarachnoid space

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10
Q

It produces the CSF

A

Choroid plexuses

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11
Q

It reabsorbs CSF

A

Arachnoid villi/ granulation

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12
Q

How many mL of CSF fluid is produced every 1 hour

A

20 mL

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13
Q

Fxn of CSF

A

✓Supply nutrients
✓Remove metabolic wastes
✓Mechanical barrier to cushion the brain and spinal cord

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14
Q

A tight fitting juncture structure of endothelial cell that protects the brain from chemicals anf other substances

A

BBB

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15
Q

Normal value of CSF

A

Adult: 90-150 mL
Neonates: 10-60 mL

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16
Q

Normal color of CSF

A

Colorless

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17
Q

Normal VISCOSITY of CSF

A

Smae with water

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18
Q

Normal SG of CSF

A

1.006 to 1.008

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19
Q

Normal pH of CSF

A

7.30 to 7.45

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20
Q

Normal pressure of CSF

A

50 to 200 mm H2O

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21
Q

3 collection tubes for CSF

A

Tube 1: chemistry and Serology
Tube 2: microbiology
Tube 3: hematology and cell count

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22
Q

Sequence of tubes when there is ONLY 1 tube

A

Microbiology: tube 2
Hematology: tube 3
Chemistry: tube 1

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23
Q

Preservation of tubes for CSF

A

Tube 1: frozen
Tube 2: room temp
Tube 3: refrigerated

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24
Q

Presence of RBC degradation product

A

Xantochromic CSF

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25
____ of WBCs disintegrates within 2hours therefore it must be preformed immediately
40%
26
T or F: CSF collection tube for cell counts shoul be refrigerated
True
27
Routine performed on CSF
Wbc count
28
How to differentiate PMNs to mononuclear cells
3% glacial acetic acid with methylene blue
29
Normal value of WBC count
Adult: 0-5 wbc/uL Neonates: 0-30 wbc/uL
30
Done only in cases of traumatic tap to correct total protein and wbc count
RBC cell count
31
How to calculate the RBC count
Total count- WBC count= RBC count
32
Diluent used for total cell count
Normal saline solution
33
May indicate presence of WBC , RBC, protein, or bacteria
Cloudy CSF
34
May be due to subarachnoid hemorrhage or traumatic tap
Bloody CSF
35
Reference csf glucost
65 mg/dL
36
Blood glucose shoul be drawn
2 hrs prior to spinal tap
37
Csf glucose and lactate is normal to
Viral meningitis
38
Normal value of csf lactate
10-24 mg/dL
39
It is produced form ammonia and alpha- ketoglutarate by the brain cells
Csf glutamine
40
When there is an Excess ammonia in csf, use......
Indirect test
41
Normal value for csf glutamine
8-18 mg/dL
42
Elevated level of csf glutamine are associated with
Reye's syndrome and hepatic ancephalopathy
43
Indicates recent destruction of myelin sheath
Myelin basic protein
44
Measurement use for myelin basic protein
Immunoassay techniques
45
By measuring the amount myelin basic protein, it could monitor
Course of multiple sclerosis
46
Detection of oligoclonal bands
Csf electrophoresis
47
Total cell count uses
Normal saline (3% acetic acid with methylene blue)
48
Dilution
Slightly hazy= 1:10 Hazy= 1:20 Slightly cloudy= 1:100 Cloudy slightly bloody= 1:200 Bloody turbid= 1:10,000
49
Distribution of blood in traumatic tap and intracranial hemorrhage
TT: uneven IH: even
50
Clot formation in traumatic tap and intracranial hemorrhage
TT: pos IH: neg
51
Supernatant of traumatic tap and intracranial hemorrhage
TT: clear IH: xanthochromic
52
Erythrophages and D-dimer of traumatic tap and Intracranial hemorrhages
TT: neg IH: pos
53
The amount of blood decreases or clears progressively
Traumatic tap
54
T or F: In traumatic tap, the CSF clots
True
55
T or F: There is NO presence of hemosiderin in traumatic tap
True
56
Addition of ______ to 0.1 mL of CSF can produce an adequate cell yield
30% albumin
57
Key word for cytocentrifugation
Monolayer
58
Normal wbc in csf
Lymphocyte and monocytes
59
Term for thr in increase in number of normal cell in csf (abnormal condition)
Pleocytosis
60
Most frequently performed chemical test
Csf protein
61
Normal values of csf protein
15-45 mg/dL
62
Major csf protein
Albumin
63
T or F: Presence of TAU protein indicates true csf
True
64
Reagent of choice because it will precipitate BOTH albumin ang globulin equally
TCA
65
2 or more oligoclonal band in csf is valuable for
Multiple sclerosis
66
Ohter neurologic disorder in csf electrophoresis
Multiple sclerosis Neutrosyphilis Encephalitis Guillain-Barre Syndrome [ MS NEG]
67
Glutamine test is frequently requested fro px with
Coma
68
It detects gram neg bacterial endotoxin
Lumulus lysate test
69
Reagent of limulus lysate test
Blood of horseshoe crab
70
Positive result in limulus lysate test
Clumping or clot formation
71
Serologic test for detection of bacterial antigens
Latext agglutination and ELISA
72
Recommended for detection of neurosyphilis
VDRL
73
Most common cause of false positive rxt in latex agglutination test is the interference of
Rheumatoid factor
74
Formula of totat cell count
CD over AD #of counted cells x dilution factor Sq counted x vol of 1sq
75
Csf serum albumin index < 9 represents
Intact bbb
76
Csf serum albumin index > 9 represents
Disrupted bbb
77
IgG index of > 0.70 indicates
IgG production within the CNS
78
Creatinine kinase of < 17 mg/mL predicts
Recovery
79
Capillary networks that form the CSF from plasma
Choroid plexus
80
Csf serum albumin index evaluates
Integrity of BBB

Cm (10 decks)

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