SEMENALYSIS Flashcards

1
Q

in collection, abstinence of ___ days

A

2-7 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

methods of collection

A

Masturbation
Coitus interruptus
Condom method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

best method of collection

A

masturbation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

method of collection which is not reliable or aka the withdrawal method

A

Coitus interruptus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

the condom method uses non-lubricant-containing rubber or _____ condom

A

polyurethane condom

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

specimen should be delivered to the lab within ___ of collection

A

1 hour

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

in collection, you should take note of the time of this three

A

specimen collection
specimen received
liquefaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

liquefaction time is usually takes ___

A

10-30 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

specimen should be kept at what temp

A

37C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

reasons for seminal fluid analysis

A

Fertility testing
Post vasectomy semen analysis
Forensic analysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

most common reason for seminal fluid analysis

A

fertility testing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

post vasectomy semen analysis is done how long after the procedure

A

2 months

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

composition of semen

A

5% spermatozoa
60-70% seminal fluid
20-30% prostate fluid
5% bulbourethral gland

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

site of spermatogenesis

A

seminiferous tubules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

seminiferous tubules are also known as

A

testes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

site of sperm maturation

A

Epididymis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

propel sperm to ejaculatory ducts

A

Vas deferens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

spermatogenesis and sperm maturation takes ___ days

A

90 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

provides nutrients for sperm and fluid

A

seminal vesicles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

secretions rich in ____ = for sperm motility

A

fructose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

acidic fluid contains ___, ___, _____ and other enzymes

A

Acid phosphatase enzyme (ACP), zinc, citric acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Prostate fluid , for _____ and ____

A

Coagulation and Liquefaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

secretes thick alkaline mucus, neutralized acidity from the prostatic secretions and vagina

A

bulbourethral gland

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

stages of sperm maturation

A
  1. Spermatogium
  2. 1° Spermatocyte
  3. 2° Spermatocyte
  4. Spermatid
  5. Spermatozoon
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Semen Appearance: Gray-white, translucent (with musty of bleach odor)
Normal
26
Semen Appearance: Increased white turbidity
Infection (increase WBC)
27
Semen Appearance: Red or Brown coloration
Blood
28
Semen Appearance: Yellow coloration
medication, abstinence, urine contamination
29
Semen normal volume
2-5 mL
30
Semen increased volume means
↑ abstinence, hyperspermia
31
Semen Decreased volume means
incomplete collection, infertility
32
Semen Viscosity Normal
pour in droplets
33
Semen Viscosity Abnormal
>2cm long threads
34
Reporting of Semen Viscosity: watery = ?
0
35
Reporting of Semen Viscosity: gel like =
4
36
Reporting of Semen Viscosity: may also be reported as ___, normal, or ___
slow, normal, or high
37
Semen pH normal
7.2-8.0
38
Semen ↑ pH = ?
infection
39
Semen ↓ pH = ?
↑ prostate fluid
40
pH should be measured within ___ hour of ejaculation
1 hour
41
Sperm concentration. Normal value = ?
>20 million sperms/mL
42
Sperm Concentration. Methods
1. Improved Neubauer Counting Chamber 2. Markler Counting Chamber
43
Improved Neubauer Counting Chamber. Dilution= ?
1:20
44
Improved Neubauer Counting Chamber. Diluents purpose
to immobilize the sperm
45
Improved Neubauer Counting Chamber. 5 Diluents
1. Formalin 2. Sodium bicarbonate 3. Saline 4. Distilled water 5. Cold tap water
46
sperm concentration method used for undiluted sperm
Markler Counting Chamber
47
Markler Counting Chamber uses __ to immobilize sperms
heat
48
Sperm Concentration Computation is the ____ method
shortcut method
49
2 WBC squares = no of sperms counted x ____
100,000
50
5 RBC squares = no. of sperms counted x _____
1,000,000
51
Standard Neubauer Formula is the ___ method
long
52
Formula: Sperm concentration (per mL) =
No. of sperms counted x dilution/ area x depth
53
Post vasectomy semen analysis is done after ___ months of surgery
2 months
54
Sperm Count. Normal Value=
>40 million sperms per ejaculate
55
formula for sperm count
sperm concentration x specimen volume
56
Sperm Motility. Normal value within 1 hour= ?. Quality= ?
>50% motile >2.0
57
Rapid, straight line motility. WHO CRITERIA AND GRADING?
a/ 4.0
58
Slower speed, some lateral movement. WHO CRITERIA AND GRADING?
b/3.0
59
Slow forward progression, noticeable lateral movement
c/20
60
No forward progression. WHO CRITERIA AND GRADING?
d/1.0
61
No movement. WHO CRITERIA AND GRADING?
0/e
62
sperm moving linearly or in a large circle. grading?
Progressive Motility (PM)
63
Sperm moving with an absence in progression. Grading??
Nonprogressive Motility (NP)
64
No movement. Grading (alternative)?
Immotility
65
determines sperm concentration, morphology, velocity, and trajectory
Computer-Assisted Semen Analysis (CASA)
66
Sperm Morphology. Normal value= ?
>30% normal forms
67
Sperm morphology. Used to measure the head, neck, tail?
Micrometer or Morphometry
68
width of sperm
3um
69
sperm head size
5um
70
sperm midpiece size
7um
71
sperm tail (flagellum) size
45um
72
head of sperm consists of ___, ___ ,and __
acrosome, cell membrane, nucleus
73
sperm midpiece consists of ___
mitochondria
74
sperm acrosomal cap is ___ of the head
1/2 of the head
75
sperm acrosomal cap is ___ of the nucleus
2/3 of the nucleus
76
the ___ is the thickest part of the sperm tail
neck
77
in preparing smears use what degree angle
45
78
stains for sperm morphology
Papanicolaou's stain Wright's stain Giemsa stain Shorr stain
79
Sperm Head. Normal = ?
oval head
80
Sperm head. abnormal shape causes?
poor ovum penetration
81
Tail abnormal shape causes?
poor motility
82
hardening of veins that drains the testes. It is the most common cause of infertility
Varicocele
83
Varicocele. Shape of sperm head?
Tapered
84
sperm morphology without tail
spermatid
85
Modified Blom's Test is used to test the ___ of sperm?
Viability
86
Modified Blom's Test should be done within __ hour of ejaculation
1 hour
87
reagents of Modified Blom's Test
Eosin + Nigrosin
88
Modified Blom's Test. Living sperms color
unstained
89
Modified Blom's Test. Dead sperms color
Red
90
Modified Blom's Test. Normal value
> 50% living sperms
91
Sperm fluid fructose is tested within __ hours
2 hours
92
Sperm fluid fructose specimen is stores ___ to prevent fructolysis
frozen
93
screening test for sperm fluid fructose
Resorcinol/ Seliwanoff's Test
94
Seliwanoff's Test positive color
orange-red color
95
when performing fertility testing, WHO recommends that _ or _ samples be collected
2 or 3
96
motile sperm can be detected for up to __ hours after intercourse
24 hours
97
non-motile sperm can persist for __
3 days
98
as the sperm die off, the __ remains and may be present for 7 days after intercourse
head
99