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AUBF > SEMENALYSIS > Flashcards

SEMENALYSIS Flashcards

1
Q

in collection, abstinence of ___ days

A

2-7 days

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2
Q

methods of collection

A

Masturbation
Coitus interruptus
Condom method

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3
Q

best method of collection

A

masturbation

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4
Q

method of collection which is not reliable or aka the withdrawal method

A

Coitus interruptus

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5
Q

the condom method uses non-lubricant-containing rubber or _____ condom

A

polyurethane condom

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6
Q

specimen should be delivered to the lab within ___ of collection

A

1 hour

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7
Q

in collection, you should take note of the time of this three

A

specimen collection
specimen received
liquefaction

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8
Q

liquefaction time is usually takes ___

A

10-30 mins

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9
Q

specimen should be kept at what temp

A

37C

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10
Q

reasons for seminal fluid analysis

A

Fertility testing
Post vasectomy semen analysis
Forensic analysis

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11
Q

most common reason for seminal fluid analysis

A

fertility testing

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12
Q

post vasectomy semen analysis is done how long after the procedure

A

2 months

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13
Q

composition of semen

A

5% spermatozoa
60-70% seminal fluid
20-30% prostate fluid
5% bulbourethral gland

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14
Q

site of spermatogenesis

A

seminiferous tubules

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15
Q

seminiferous tubules are also known as

A

testes

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16
Q

site of sperm maturation

A

Epididymis

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17
Q

propel sperm to ejaculatory ducts

A

Vas deferens

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18
Q

spermatogenesis and sperm maturation takes ___ days

A

90 days

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19
Q

provides nutrients for sperm and fluid

A

seminal vesicles

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20
Q

secretions rich in ____ = for sperm motility

A

fructose

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21
Q

acidic fluid contains ___, ___, _____ and other enzymes

A

Acid phosphatase enzyme (ACP), zinc, citric acid

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22
Q

Prostate fluid , for _____ and ____

A

Coagulation and Liquefaction

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23
Q

secretes thick alkaline mucus, neutralized acidity from the prostatic secretions and vagina

A

bulbourethral gland

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24
Q

stages of sperm maturation

A
  1. Spermatogium
  2. 1° Spermatocyte
  3. 2° Spermatocyte
  4. Spermatid
  5. Spermatozoon
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25
Q

Semen Appearance: Gray-white, translucent (with musty of bleach odor)

A

Normal

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26
Q

Semen Appearance: Increased white turbidity

A

Infection (increase WBC)

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27
Q

Semen Appearance: Red or Brown coloration

A

Blood

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28
Q

Semen Appearance: Yellow coloration

A

medication, abstinence, urine contamination

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29
Q

Semen normal volume

A

2-5 mL

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30
Q

Semen increased volume means

A

↑ abstinence, hyperspermia

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31
Q

Semen Decreased volume means

A

incomplete collection, infertility

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32
Q

Semen Viscosity Normal

A

pour in droplets

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33
Q

Semen Viscosity Abnormal

A

> 2cm long threads

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34
Q

Reporting of Semen Viscosity:
watery = ?

A

0

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35
Q

Reporting of Semen Viscosity: gel like =

A

4

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36
Q

Reporting of Semen Viscosity: may also be reported as ___, normal, or ___

A

slow, normal, or high

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37
Q

Semen pH normal

A

7.2-8.0

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38
Q

Semen ↑ pH = ?

A

infection

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39
Q

Semen ↓ pH = ?

A

↑ prostate fluid

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40
Q

pH should be measured within ___ hour of ejaculation

A

1 hour

41
Q

Sperm concentration. Normal value = ?

A

> 20 million sperms/mL

42
Q

Sperm Concentration. Methods

A
  1. Improved Neubauer Counting Chamber
  2. Markler Counting Chamber
43
Q

Improved Neubauer Counting Chamber. Dilution= ?

A

1:20

44
Q

Improved Neubauer Counting Chamber. Diluents purpose

A

to immobilize the sperm

45
Q

Improved Neubauer Counting Chamber. 5 Diluents

A
  1. Formalin
  2. Sodium bicarbonate
  3. Saline
  4. Distilled water
  5. Cold tap water
46
Q

sperm concentration method used for undiluted sperm

A

Markler Counting Chamber

47
Q

Markler Counting Chamber uses __ to immobilize sperms

A

heat

48
Q

Sperm Concentration Computation is the ____ method

A

shortcut method

49
Q

2 WBC squares = no of sperms counted x ____

A

100,000

50
Q

5 RBC squares = no. of sperms counted x _____

A

1,000,000

51
Q

Standard Neubauer Formula is the ___ method

A

long

52
Q

Formula: Sperm concentration (per mL) =

A

No. of sperms counted x dilution/ area x depth

53
Q

Post vasectomy semen analysis is done after ___ months of surgery

A

2 months

54
Q

Sperm Count. Normal Value=

A

> 40 million sperms per ejaculate

55
Q

formula for sperm count

A

sperm concentration x specimen volume

56
Q

Sperm Motility. Normal value within 1 hour= ?. Quality= ?

A

> 50% motile
2.0

57
Q

Rapid, straight line motility. WHO CRITERIA AND GRADING?

A

a/ 4.0

58
Q

Slower speed, some lateral movement. WHO CRITERIA AND GRADING?

A

b/3.0

59
Q

Slow forward progression, noticeable lateral movement

A

c/20

60
Q

No forward progression. WHO CRITERIA AND GRADING?

A

d/1.0

61
Q

No movement. WHO CRITERIA AND GRADING?

A

0/e

62
Q

sperm moving linearly or in a large circle. grading?

A

Progressive Motility (PM)

63
Q

Sperm moving with an absence in progression. Grading??

A

Nonprogressive Motility (NP)

64
Q

No movement. Grading (alternative)?

A

Immotility

65
Q

determines sperm concentration, morphology, velocity, and trajectory

A

Computer-Assisted Semen Analysis (CASA)

66
Q

Sperm Morphology. Normal value= ?

A

> 30% normal forms

67
Q

Sperm morphology. Used to measure the head, neck, tail?

A

Micrometer or Morphometry

68
Q

width of sperm

A

3um

69
Q

sperm head size

A

5um

70
Q

sperm midpiece size

A

7um

71
Q

sperm tail (flagellum) size

A

45um

72
Q

head of sperm consists of ___, ___ ,and __

A

acrosome, cell membrane, nucleus

73
Q

sperm midpiece consists of ___

A

mitochondria

74
Q

sperm acrosomal cap is ___ of the head

A

1/2 of the head

75
Q

sperm acrosomal cap is ___ of the nucleus

A

2/3 of the nucleus

76
Q

the ___ is the thickest part of the sperm tail

A

neck

77
Q

in preparing smears use what degree angle

A

45

78
Q

stains for sperm morphology

A

Papanicolaou’s stain
Wright’s stain
Giemsa stain
Shorr stain

79
Q

Sperm Head. Normal = ?

A

oval head

80
Q

Sperm head. abnormal shape causes?

A

poor ovum penetration

81
Q

Tail abnormal shape causes?

A

poor motility

82
Q

hardening of veins that drains the testes. It is the most common cause of infertility

A

Varicocele

83
Q

Varicocele. Shape of sperm head?

A

Tapered

84
Q

sperm morphology without tail

A

spermatid

85
Q

Modified Blom’s Test is used to test the ___ of sperm?

A

Viability

86
Q

Modified Blom’s Test should be done within __ hour of ejaculation

A

1 hour

87
Q

reagents of Modified Blom’s Test

A

Eosin + Nigrosin

88
Q

Modified Blom’s Test. Living sperms color

A

unstained

89
Q

Modified Blom’s Test. Dead sperms color

A

Red

90
Q

Modified Blom’s Test. Normal value

A

> 50% living sperms

91
Q

Sperm fluid fructose is tested within __ hours

A

2 hours

92
Q

Sperm fluid fructose specimen is stores ___ to prevent fructolysis

A

frozen

93
Q

screening test for sperm fluid fructose

A

Resorcinol/ Seliwanoff’s Test

94
Q

Seliwanoff’s Test positive color

A

orange-red color

95
Q

when performing fertility testing, WHO recommends that _ or _ samples be collected

A

2 or 3

96
Q

motile sperm can be detected for up to __ hours after intercourse

A

24 hours

97
Q

non-motile sperm can persist for __

A

3 days

98
Q

as the sperm die off, the __ remains and may be present for 7 days after intercourse

A

head

99
Q
A

AUBF (8 decks)

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