SECTIONS OF LABORATORY AND COMMONLY REQUESTED LABORATORY TESTS Flashcards
- This section is intended for the testing of blood and other body fluids to quantify essential soluble CHEMICALS including waste products useful for the diagnosis of certain diseases
- analyzes serum and plasma for chemical constituents to evaluate general health and disorders of body systems and organs
Clinical chemistry
- for the diagnosis of diabetes mellitus
Fasting Blood Sugar (FBS) and Glycosylated Hemoglobin
- High- and Low-Density Lipoprotein
- Triglycerides
Total Cholesterol
For the diagnosis of cardiovascular diseases
triglycerides
Elevated levels indicate kidney disorders or gout
blood uric acid
Elevated levels indicate kidney disorders
blood urea nitrogen
Elevated levels indicate kidney disorders
Creatinine
ALT means?
Alanine aminotransferase
- Elevated levels indicate liver disorders
Aspartate aminotransferase
- Elevated levels indicate BONE or LIVER disorders
Alkaline phosphatase
- Elevated levels indicate liver or hemolytic disorders
Bilirubin
Elevated levels indicate early liver disorders
Gamma-glutamyltransferase (GGT)
Elevated levels indicate myocardial infarction or lung or liver disorders
Lactic dehydrogenase
Elevated level indicate pancreatitis.
Amylase and lipase
Evaluate body fluid balance
Electrolyte (Na, K, Cl, CO2)
This section is subdivided into
four sections:
* Bacteriology
* Mycobacteriology
* Mycology and Virology
- Focused on the identification of bacteria and fungi on specimens received.
* Most samples are obtained from the blood, urine, throat, sputum, genitourinary tract, wounds, cerebrospinal fluid and feces
Microbiology
4 steps of primary stain
- Crystal violet
- Iodine
- Alcohol
- Safranin
- This section deals with the enumeration of cells in the blood and other body fluids (e.g., CSF, pleural fluid, etc.)
- Coagulation studies focus on blood testing for the determination of various coagulation factors
Hematology and Coagulation Studies
✓ the most common body fluid analyzed in this section and a specimen obtained by using a collection tubes with lavender stopper that contain ethylenediaminetetraacetic acid (EDTA) which inverted eight times.
✔The liquid portion of the blood is PLASMA if it is obtained from a sample that has been anticoagulated. Contains protein fibrinogen
✓If the sample is allowed to clot, the liquid portion is SERUM
Whole blood
- Is the most common type of test performed in the Hematology section
- Screening test to assess patient conditions such as INFECTIONS and MALIGNANCY.
Complete Blood Count
Determines the percentage of the different types of WBC and evaluates RBC and platelet morphology
differential
Determines the volume of RBC packed by CENTRIFUGATION
hematocrit
Determines the OXYGEN-carrying capacity of RBC
hemoglobin
Determines the hemoglobin in RBC amount of
Mean corpuscular hemoglobin
Determines the weight of hemoglobin in RBC and compares it with the size of the cell
Mean corpuscular hemoglobin
concentration
Determines the size of RBC
Mean corpuscular volume
Determines the number of platelets in circulating blood
Platelet count
Determines the number of RBC in circulating blood
Red blood cell count
Calculation to determine
differences in the size of RBC
Red blood cell distribution width
Determines the number of WBC in circulating blood
White blood cell count
Determines the number and type of cells in bone marrow
Body fluid analysis
Determines the number and type of cells in bone marrow
Bone marrow
Determines the rate of RBC sedimentation
Erythrocyte Sedimentation Rate
Evaluates bone marrow production of RBC
Reticulocyte count
Screening test for sickle cell anemia
Sickle cell
- In this area, the overall process of Hemostasis (stoppage of blood flow from a damaged blood vessel) is evaluated; this includes platelets, blood vessels, coagulation factors, fibrinolysis, inhibitors and anticoagulant therapy
- Plasma from a sample drawn in tube with light blue stopper that contains the anticoagulant sodium citrate
Coagulation Section
Evaluates the intrinsic system of coagulation cascade and monitors heparin therapy
Activated Partial Thromboplastin Time
Screening test for increased clotting tendencies
Antithrombin III
Evaluate the function of platelets
Bleeding time
Measures abnormal blood clotting and fibrinolysis
D-dimer
Detect factor deficiencies that prolong coagulation
Factor Assays
Test for increased fibrinolysis
Fibrin degradation products
Determines the amount of fibrinogen in plasma
Fibrinogen
Evaluates the function of platelets
Platelet aggregation
Evaluates the extrinsic system of coagulation cascade and monitors Coumadin therapy
Prothrombin time and international normalized ratio
Determines if adequate fibrinogen is present for normal coagulation
Thrombin time
- This section comprise two areas:
* Urine analysis and other body fluids
* Examination of stool or routine fecalysis
* Urinalysis is the routine screening procedure to detect disorders and infections of the kidney and to detect metabolic disorder such as diabetes mellitus and liver disease.
* Consists of physical, chemical, and microscopic examination in urine
Clinical microscopy
In urinalysis, this…
- detects blood, bilirubin and other pigments
- Depends on hydration status
- Normal color of urine = Yellow
color
In urinalysis, this:
Detects cellular and crystalline elements
Appearance
In urinalysis, this:
Measures the concentration of urine
Specific gravity
In urinalysis, this: Determines the acidity of urine
pH
In urinalysis, this: Elevated levels indicates KIDNEY DISORDERS
Protein
In urinalysis, this: Elevated levels indicates DIABETES MELLITUS
Glucose
In urinalysis, this: Detects red blood cells or hemoglobin
Blood
In urinalysis, Elevated levels indicate LIVER DISORDERS
Bilirubin
In urinalysis, Elevated levels indicate LIVER or HEMOLYTIC DISORDER
Urobilinogen
In urinalysis, this: Detects BACTERIAL INFECTION
Nitrite
In urinalysis, this: Detects WBC
Leukocyte esterase
In urinalysis, this determines the number and type of CELLULAR ELEMENTS (elements include: RBC, WBC, Bacteria, casts, crystals, other forms of crystal elements)
Microscopic
- Blood typing and compatibility testing are the two main activities performed in this section.
- Screening for all ANTIBODIES and identification of antibodies as well as blood components used for transfusion are also conducted.
- Blood bank samples are collected in plain red, lavender, or pink stopper plasma.
Immunohematology/blood banking
Refers to: ABO and Rh (Rehus) typing
(Blood) Group and Type
Detects abnormal antibodies in serum
Antibody screen
Detects abnormal antibodies on RBCs
Direct antihuman globulin test or direct Coombs
Identifies abnormal antibodies in serum
Panel
ABO, Rh typing, and compatibility test
Type and crossmatch
ABO, Rh typing, and compatibility test
Type and screen
- Performs tests to evaluate the body’s immune response; that is, the production of antibodies and cellular activation
- Analyses of SERUM ANTIBODIES in certain infectious agents (primarily viral agents) are performed in this section
- Hepatitis B profile tests, serological test for syphilis, and tests for hepatitis C and dengue fever some examples of antibody screening tests
- Blood for this section is collected with Cred stoppers.
Immunology/Serology
Study of immune system
Immunology
Study of serum
serology
Liquid portion of clotted blood
serum
liquid portion of unclotted/anticoagulated blood
plasma
most abundant coagulation protein in the blood
fibrinogen
HbA1C
glycosylated hemoglobin
- total cholesterol
- HDL - high density lipoprotein
- LDL - low density lipoprotein
- triglycerides
lipid profile
fasting time range for fasting blood sugar?
8-10 hrs
Fasting time range for lipid profile?
12 - 14 hrs
high lvls of sodium
hypernatremia
low lvls of sodium
hyponatremia
high lvls of potassium
hyperkalemia
low lvls of potassium
hypokalemia
high lvls of chlorine
hyperchloremia
low lvls of chlorine
hypochloremia