Biosafety and Security (PPT) Flashcards
- Any microbial entity, cellular or non-cellular structurally, occurring or engineered, capable of replication or of transferring genetic material that may be able top provoke infection, allergy, toxicity, or other adverse effects in humans, animals, or plants (ISO 35001:2019)
- example: HIV, SARS-COV2
Biological agent
- Any material comprised of, containing, or that may contain biological agents and/or their harmful products such as toxins and allergens (ISO 35001:2019)
- example: blood in the test tube, sputum in the mask
biological material
potential source of harm caused by biological materials (ISO 35001:2019)
biohazard
containment principles, technologies, and practices implemented to prevent unintentional/accidental exposure to pathogens and toxins, or their unintentional/accidental release (WHO laboratory manual 3rd edition)
laboratory biosafety
institutional and personal security measures designed to prevent that loss, theft, misuse, diversion, or intentional release of pathogens and toxins (WHO laboratory manual 3rd edition)
laboratory biosecurity
Biosafety or biosecurity?
recapping of needles
biosafety
Biosafety or biosecurity?
medtech is a member of terrorist group
biosecurity
Biosafety or biosecurity?
lab personal is not provided with PPE
biosafety
Biosafety or biosecurity?
disgruntled staff
biosecurity
Biosafety or biosecurity?
workload overload
Biosafety
Biosafety or biosecurity?
waste from lab are not segregated
biosafety
Biosafety or biosecurity?
lack of staff training
biosafety
Biosafety or biosecurity?
staff with a huge debt
biosafety (lack of prioritization on procedures performed) and biosecurity (may risk the pathogens for selling to settle debt)
Organizations in the field of biosafety:
* Internationally: ABSA, EBSA, A-PBA
What are the names of these abbreviations?
- American Biological Safety Association (ABSA)
- European Biological Safety Association (EBSA)
- Asia-Pacific Biosafety Association (A-PBA)
Organizations in the field of biosafety:
* locally: PhBBA, and BRAP
PhBBA, and BRAP means?
- Philippine Biosafety and Biosecurity Association (PhBBA)
- Biological Risk Association of the Philippines (BRAP)
Difference between PhBBA and BRAP?
PhBBA is a govt institution and aids DOH and DOA. Meanwhile, BRAP is a NGO
– Most important concept/ strict adherence
– Aware of potential hazard
– Trained and proficient in techniques
– Supervisors responsible for appropriate laboratory facilities, personnel and training
standard practices
Includes:
– Occupational health programs
special practices and procedures
What is under the first principle of biosafety, A. Practice and procedures?
- standard practices
- special practices and procedures
– Primary containment barrier
– Minimize exposure to hazard (prevent contact/contain aerosols)
– Engineering controls, equipment, PPE, Biosafety cabinet, covered animal cage system, sealed centrifuge rotors
Safety and equipment
– Secondary barrier
– Protects outside the laboratory
– Building and lab design, ventilation, autoclaves, cage wash facility, sealed laboratory walls/floors, HEPA filters
facility design and construction
also referred as Containment levels. It is the combination of laboratory practices and procedures, safety equipment (primary barriers) and laboratory facilities (secondary barriers)
biosafety levels
- Student training and teaching laboratory
- Well-defined and characterized agents
– Nonpathogenic organism
❑Bacillus subtilis, non pathogenic strain of E.coli
❑Naegleria gruberi
❑Saccharomyces cerevisiae - No harm to humans
- Relies on standard microbiology practices
Biosafety Level 1
Practices
* Standard microbiological practices are followed.
* Work can be performed on an open lab bench or table
Biosafety level 1