Schultz-Oct 9 Flashcards
1
Q
chromatin
A
DNA + histones + any other proteins associated with DNA
2
Q
nucleosome
A
histone core + DNA wrapped around in histone core
3
Q
nucleosome core particle
A
H2A, HB, H3, H4
4
Q
how many copies of H2A/H2B dimers in nucleosome core?
A
2
5
Q
How many copies of H3/H4 tetramer in nucleosome core?
A
1
6
Q
3 histone chaperones
A
CAF1, Asf1, and NAP1
7
Q
Where does CAF1 and Asf1 bind on histone?
A
H3-H4
8
Q
Where does NAP1 bind n histone?
A
H2A-H2B
9
Q
Function of histone chaperones
A
promote initial formation of histone-DNA complex
10
Q
3 DNA-dependent ATPases
A
ACF, RSF, Chd1
11
Q
Which 2 DNA-dependent ATPases need chaperones?
A
ACF and RSF
12
Q
Which chaperone shield histone surfaces that interact with DNA?
A
Nap1
13
Q
What do you get on gel when you do not use ATP in chromatin assembly?
A
- monosomes, but no disomes or higher order ones
