Glover-1 Pt. 2

Question 1

Function of mediator

Answer 1

• Binds CTD and makes stable complex with RNAPII

- Target of regulatory factors

Question 2

Function of TFIIB

Answer 2

• Its CTD interacts with C-terminal stirrup of TBP and DNA upstream and downstream of TBP
• Interacts with BRE

Question 3

Function of interaction between TFIIB and BRE

Answer 3

• ensures TFIIB-TBP complex is properly oriented

Question 4

What part of TFIIB binds RNAPII

Answer 4

N terminal domain

Question 5

Function of TFIIB-RNAPII interaction

Answer 5

correctly positions RNAPII at start site of transcription

Question 6

Function of TFIIA

Answer 6

• Binds to N-terminal stirrup of TBA and DNA upstream TATA box
• stabilizes interactions w/ TATA box

Question 7

Function of TFIIH

Answer 7

• DNA-dependent ATPase

- ATP-dependent helicase

Question 8

Fe-BABE

Answer 8

• iron plus a protein

- has carboxylated group bound to Fe

Question 9

Fenton Chemistry

Answer 9

H2O2 –(Fe2+-> HO radical (unpaired e-)