Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIOCH 430 > Glover-2 > Flashcards

Glover-2 Flashcards

1
Q

Which groove in DNA has more specificity?

A

Major

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Which basepairs are recognized by Arg sidechains?

A

G-C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Major classes/families of eukaryotic TFs (x5)

A
  • homeodomains
  • Zn fingers
  • bZIP
  • bHLH
  • nuclear receptors
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Structure of homeodomains

A
  • 3 alpha helices (2 of them are C terminal)

- 1 N-terminal strand

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is Helix-turn-Helix (HTH) motif usu. associated with?

A

DNA binding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Function of last C terminal helix in homeodomain

A

sequence-specific interaction with approx. 4 bps

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Function of N-terminal domain of homeodomain

A

interaction with minor groove –> less specific

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the sequence motifs for Zn fingers?

A

Finds 2 Cys and 2 His

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How many bZIPs does it take to bind DNA?

A

2 - dimer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What does bZIP stand for?

A

Basic Leucine Zipper

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Where are the basic residues on bZIP?

A

N-terminal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Structure of C terminal of bZIP

A

has Leu for every 7th residue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

When does the basic region adopt its helical structure?

A

When it contacts DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What does bHLH stand for?

A

basic Helix Loop Helix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the nature of bHLH when it binds DNA?

A

dimers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Where is the basic region for bHLH?

A

N terminal

17
Q

What does C terminal look for bHLH?

A

dimerizes into 4 alpha helix bundles

18
Q

What activates nuclear receptors?

A

hydrophobic ligands

19
Q

What molecule(s) is/are in the DNA binding domain of nuclear receptors?

A

2 Zn atoms

20
Q

What makes the sequence specific contacts in nuclear receptors?

A

alpha helix in major groove of DNA

21
Q

What is the binding portion of Rel?

A

a pair of linked beta sheet structures

22
Q

What terminal is the DNA binding portion of Rel?

A

N-terminal

23
Q

What is the dimerization domain of Rel?

A

C terminal

24
Q

What part of the N terminal of Rel makes contact with DNA?

A

polypeptide loop

25
Q

What part of p53 is the DNA binding domain?

A

Central

26
Q

What part of p53 is the tetramerization domain?

A

C-terminal

27
Q

How does p53 usually bind to DNA?

A

as a tetramer

28
Q

What structure does p53’s central binding domain adopt when it binds to DNA?

A

Ig fold

29
Q

What specific structures of p53 bind to:

  • major groove
  • minor groove
A
  • loop and alpha helix

- another peptide loop

30
Q

What does multimeric TFs mean?

A

large protein complexes made up of many different regulatory proteins

31
Q

What is the dimerization element of Fos and Jun?

A

coiled coil

32
Q

Where in the coiled coil does it contain hydrophobic residues?

A

a and d

33
Q

Where in the coiled coil does it contain charged side chains?

A

e and g

BIOCH 430 (11 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions