Sample Collection and Preservation (M) Flashcards
After sx (tissue) collection, what should be done?
Tissue should be fixed immediately
The tissue should be fixed immediately in what fixative?
10% neutral buffered formalin (NBF)
The formalin (10% NBF) causes what?
1) Chemical and physical changes
2) Hardens and preserve the tissue
3) Protect from degeneration
4) Protect from autolysis
5) Protect from putrefaction
What is the proper sx to fixative ratio?
1:3
1 = sx 3 = 10% NBF
3 times the volume of fixative in ratio to the size of the sx
True or False
Fixative is 20 - 30 times greater than the size of the tissue
False, because fixative is 10 - 20 times greater than the size of the tissue
True or False
Tagging of the sx is highly recommended
True
True or False
It is an optional choice for every institution to have evolve tagging mechanism
False, because every institution should evolve tagging mechanism
Provide an ex (/ importance) of tagging done to a sx
Ex. 1 tie for right fallopian tube
Importance: The sx is clearly defined in the histopath request form
True or False
Large radical sx resection should be sliced / cut by the surgeon
False, because large radical sx resection should not be sliced / cut by the surgeon
What is loafing?
Cutting the sx into 3 or more parts
What is the importance of loafing?
If loafing is done, the fixative can easily penetrate the tissue
At what sxs can loafing be applied?
In large radical sxs
What are the exs of large radical sxs where loafing can be done?
1) Modified Radical Mastectomy (MRM)
2) Total Abdominal Hysterectomy & Bilateral Salphingo Oophorectomy (TAH-BSO)
What are the exs of small fragile sx?
1) Punch biopsy
2) Gastric tissues
What should be done to small fragile sxs?
These should be wrapped in a gauze envelope
