Sample Collection and Preservation (M) Flashcards

1
Q

After sx (tissue) collection, what should be done?

A

Tissue should be fixed immediately

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2
Q

The tissue should be fixed immediately in what fixative?

A

10% neutral buffered formalin (NBF)

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3
Q

The formalin (10% NBF) causes what?

A

1) Chemical and physical changes
2) Hardens and preserve the tissue
3) Protect from degeneration
4) Protect from autolysis
5) Protect from putrefaction

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4
Q

What is the proper sx to fixative ratio?

A

1:3

1 = sx
3 = 10% NBF

3 times the volume of fixative in ratio to the size of the sx

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5
Q

True or False

Fixative is 20 - 30 times greater than the size of the tissue

A

False, because fixative is 10 - 20 times greater than the size of the tissue

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6
Q

True or False

Tagging of the sx is highly recommended

A

True

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7
Q

True or False

It is an optional choice for every institution to have evolve tagging mechanism

A

False, because every institution should evolve tagging mechanism

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8
Q

Provide an ex (/ importance) of tagging done to a sx

A

Ex. 1 tie for right fallopian tube

Importance: The sx is clearly defined in the histopath request form

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9
Q

True or False

Large radical sx resection should be sliced / cut by the surgeon

A

False, because large radical sx resection should not be sliced / cut by the surgeon

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10
Q

What is loafing?

A

Cutting the sx into 3 or more parts

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11
Q

What is the importance of loafing?

A

If loafing is done, the fixative can easily penetrate the tissue

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12
Q

At what sxs can loafing be applied?

A

In large radical sxs

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13
Q

What are the exs of large radical sxs where loafing can be done?

A

1) Modified Radical Mastectomy (MRM)

2) Total Abdominal Hysterectomy & Bilateral Salphingo Oophorectomy (TAH-BSO)

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14
Q

What are the exs of small fragile sx?

A

1) Punch biopsy

2) Gastric tissues

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15
Q

What should be done to small fragile sxs?

A

These should be wrapped in a gauze envelope

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16
Q

What are the components of a test requisition form?

A

1) Pt demographics
2) Operation
3) Type/s of sx
4) No. of sx and container submitted
5) Details of history (clinical data)
6) Clinical impression