Fresh Tissue Examination (M)

Question 1

Tissue exam may vary accdg to what?

Answer 1

1) Structural and chemical components of the cells
2) Nature and amt of the tissue
3) Need for an immediate exam of a tissue structure

Question 2

What is the advantage of examining fresh tissue?

Answer 2

Protoplasmic activities can be observed / viewed

Question 3

What are the exs of protoplasmic activities?

Answer 3

1) Motion
2) Mitosis
3) Phagocytosis
4) Pinocytosis

Question 4

What is the process of teasing or dissociation?

Answer 4

Tissue sx is immersed in a watch glass w/ an isotonic saline solution (dissected or separated)

Question 5

The tissue processed via teasing is examined under what?

Answer 5

Phase contrast or bright field: if unstained

differential dyes: if stained

Question 6

What is the process of squash preparation (crushing)?

Answer 6

Small tissues of tissue (not 1 mm >) placed in 2 microscope slides and compressed forcibly

Question 7

What type of stain may be placed at the junction of the slide and cover glass w/c is absorbed by capillary action (in squash preparation)?

Answer 7

Vital stain

Question 8

What is the process (/ principle) of smear preparation?

Answer 8

Examining sections / sediments where cellular materials are spread lightly over a slide by means of a wire loop / applicator stick

Question 9

What is the purpose of smear preparation?

Answer 9

Useful in cytological exams especially for cancer dx

Question 10

What are the different types of smear preparation?

Answer 10

1) Streaking
2) Spreading
3) Pull apart
4) Touch preparation (impression smear)

Question 11

What is the process (/ principle) of streaking?

Answer 11

W/ an applicator stick / wire loop, the material is rapidly and gently applied in a direct / zigzag line

Must obtain a uniform distribution of secretion

Question 12

What should be avoided when streaking and why should these be avoided?

Answer 12

The formation of too thick / too thin smears because these are unsuitable for exam

Question 13

What is the process (/ principle) of spreading?

Answer 13

Spread into a moderately thick film by teasing the mucus strands apart w/ an applicator stick

Question 14

What is the comparison between streaking and spreading?

Answer 14

Spreading is more tedious > streaking

Question 15

What is the advantage brought by spreading?

Answer 15

It maintains cellular interrelationships

Question 16

What are recommended sxs where spreading can be done?

Answer 16

1) Fresh sputum
2) Bronchial aspirations
3) Thick mucoid secretions

Question 17

What is the process (/ principle) of pull apart?

Answer 17

Placing a drop secretion / sediment upon 1 slide over another slide

Slight movement of 2 slides in opposite directions to initiate the flow of materials, 1 single uninterrupted motion

Question 18

What are the recommended sxs where pull apart can be done?

Answer 18

1) Serous concentration
2) Sputum
3) Enzymatic lavage from GIT
4) Blood smears

Question 19

What is the process (/ principle) of touch preparation?

Answer 19

Tissue -> in contact and pressed into slide -> cells transferred directly to the slide -> examine via phase contrast microscopy or stained for light microscopic study

Question 20

What is the advantage of doing touch preparation?

Answer 20

Sx is examined w/out destroying their actual intercellular relationship