Salivary Pellicle 2 Flashcards
What does the enamel pellicle consist of
mucins PRP statherin amylase lysozyme lactoferrin Ig GTFs (bacterial) Glucans (bacterial)
what si the pellicle a a prerequisite
bacterial attachement
why are some mucin functions
tissue coating
lubrication
what is the tissue coating action of mcucins
protective hard/soft tissues
form acquired pellicle
aggregate microbes for removal
conc antimicrobial mols at interface
what si the lubrication function of mucin
alight themselves with direction of flow
increase lubricating qualities
what is the interaction MG1
attracts some sp
s.sanguinis
s.mitis
actinomyces
what si the interactions assc with MG2
attracts MANY bac sp s.sanguinis s.gordonii E.corrodens s.aureus
what are some features of MG2 and is interaction with bacteria
long protein core with short CHO chain often end in sialic acid
neurmidase cleaves sialic acid expo galactose (bac binding area)
what si the antibacterial effect of MG2
proteolytic cleavage of MG2 = peptides kill bac
anti fungal peptides assc
what si the proteolytic cleavage
release of peptides nerves from acidic, basic PRPs, statherin and histatns
what is special about proline
flexible
what are the 3 groups of PRPs
acidic
basic
Glycosylated
explain he acidic fam of PRPs
unique to saliva
affinity for HA
antic function
explain basic fam of PRPs
in saliva, nasal and bronchial sec
complex with tannic acid
what si the gycosylated fam of PRPs Imp for
newly formed pellicle
what do PRPs essentially do
stop uncontrollable remineralisation of both by occupy nucleation sites and holding Ca and Phos
give an individual who has higher levels of PRp in saliva
caries free
does caries present affect qualities of saliva
yes
caries free pt usually antibacterial proteins etc
how does the acidic fam of PRP work
bind to HA
conformational change
Terminus expo to receptor
eg A viscosus
how does the glycosylated fam of PRPs work
bind to HA
bind to s mutans efficiently
PRPs are strong promoters for what
bacterial adhesion
what are the terminals of PRPs
amino - control CaPhos
carboxyl - nterctaion with bac
what are interactions of PRPs said to be
highly sp
what do the interactions of PRPs dept on
proline-glutamie dipeptide
dont inhibit adhesion of bac when in solution
what do statherins prevent
primary and secondary CaPhos disposition
what si the c terminal of statheirn involved in
binding to pedicle and conformational change
what does sthatehrins aid
A viscous and F nucleate bind to HAp
what are the bonds in amylase
alpha 1,4 glycosidic bond
what is the function of amylase major
digestion starches
what are the other features of amylase
essential for formation of pellicle
bacterial receptor
forme heterotypic mixed micelle like structures
what is amylase a bac receptor for
s sanguines and s gorndonii
what are glucans
glucose polysaccharides with glucose molesucels joined by (alpha) 1,3/1,6 links
in formation of gluons what is the enzyme used
glucosyltransferase
what re water soluble glucans
dextrans
(1,6) alpha
what are water insoluble glucans
mutans 1,3/1,6 (alpha) links
wha is imp about pellicle attached GTFs
in pellicle
immobilised enzymes active and prod sticky polysacc
binding site for bacteria adhesion
what are Ig found in pellicle
IgA
IgG
what is the most abundant Ig found in pellicle
IgA2
shorter hinge resist protease
what do the pellicle attached Ig do
inhibit growth & adherence of some sp
antiseptic
immunise ag s.mutans
what are cystatins
inhibitors of cysteine proteases
what are cystatins linked to
pellicle formation and remineralisation
control of perio disease
protect salivary proteins from degardation
what are histatins
histidine rich cationic (+ve) peptides
what are the main properties of histatins
anti fungal - anticandidal
antibacterial - smutans
what are histamines incorp into
acquired pellicle
what are some other components of the pellicle
lysozyme
lactoferrin
albumin
what is a lysozyme
muramidase
n - acetylmuramidase
cationic protein
where are lysozymes present
in numerous organs and most body fluids
where are the sources of oral lysozymes
major & minor salivary glands, phagocytic cells and gingival crevicular fluid
what bacteria tend to be more resistant to lysosmyes and why
gm -ve
due to LPS in cel wal
what do lysozyme activate
bacterial autolysins
‘suicide packages’
what is lactoferrin
iron chelating glycoprotein
what is the principle action of lactoferrin
block growth of iron dept organisms with b’static effect
- binds to LPS
- inhibit endotoxin activity
what is HOSCN a product of
salivary peroxidase activity
what is HOSCN
enzyme adsorb onto HA
what does HOSCN do
block glucose uptake
inhibit amino acid trans
damage inner mem
inhibit hexokinase
what are some antimicrobial components of saliva
lactoferrin IgA Mucins Antimicrobial peptides Enzymes Proteins
what are th antimcirbial enzymes in saliva
lysozyme
glycosides
peroxidase
what are the antimicrobial proteins in saliva
glycoproteins
statherins
histidine rich proteins
proline rich proteins
what is the thickness of the pellicle before bac attach
1-2uM (takes an hour)
what are the factors involved in pellicle to plaque
MG1 sIgA lysozyme - all bind to bac cell walls pioneer sp (s gordonii and s sanguinis)
