Cell Cycle Flashcards
What is the pathway usually taken to get 2 identical daughter cells
Binary fission
What is the genetic info composed of in bacterial cells
Single circular chromosome
Some cases some prokaryotes have 2 chormosomes what is an example
Vibrio cholerae
What does streptomyces have for their DNA
Linear chormosome act like a circular one
What is a replicon
Portion of chromosome which is able to initiate its own replication
What is the site of initiation
OriC origin
Where does termination occur
In 100Kb region
What is the period rom initiation to completion of replication
Time C (40 mins for ecoli)
What happens to ecoli bidirectional replication
Arrests in a termination trap
What is the D time
Time after replication to divide (20 mins for ecoli)
How does availability of DnaA protein affect chromosome replication
To initiate a new round of DNA replication enough DnaA-ATP has to bind to specific DNA sequences in origin
What do dedicated proteins do
Segregate the chromosomes
What do dynamic par proteins do
Segregate the chromosomes and plamsids
What is the central event of the cell division
The assembly of the FtsZ ring
What effect does FtsZ have on division
Controls it
What are the key proteins for cell division
FtsZ, FtsI, PBP3
In production what happens
FtsI encodes PBP3 (penicillin binding protein)
What is PBP3
Transmembrane protein which cross links peptide side chains
What are some factors which are to be considered in prokaryotic growth
Food Temperature pH Osmotic pressure Oxygen
What is the bacterial cell cycle used for
New cell grows in size and splits into 2
What is the FTsZ protein
First protien to move to site of division
Essential for recruiting other proteins that produce a new cell wall between dividing cell
What si the septum
Partition that separates the cells of a divide prokaryote
What forms the z ring and what does it do
Formed from smaller subunits of FtsZ
Filaments pull on each other and tighten to divide the cell
What is binary fission
Method by which prokaryotic cells produce new individuals that are genetically identical to the parent organism
Where is the origin of replication
Close to the binding site of the chromosome at the plasma mem
