RPR/Monospot Flashcards
What is RPR?
Rapid plasma reagin
What is a non-treponemal test for serologic detection of the
antibody Reagin?
RPR
what is produced in response to an infection of syphilis?
antibody Reagin
Syphilis is caused by what?
Treponema pallidum
Reagin is found in?
serum/plasma
what is the vehicle or “antigen” to see results RPR
macroscopically.
Reagin
is a non-treponemal screening test that requires confirmation.
Rapid Plasma Reagin
If flocculation is present
reactive (positive)
no flocculation is present
non-reactive (negative)
What is the confirmatory test for RPR testing?
(FTA-ABS)
What does (FTA-ABS) stand for?
Fluorescent treponemal antibody-absorbed test
Dead T. pallidum is fixed on a slide with what?
patient’s serum
fluoresce =
positive
no fluoresce =
negative
Which tube color is collected for serum?
Red
Which tube color is collected for plasma?
Purple
After centrifugation, serum should be __________,
to be an acceptable sample.
clear and non-hemolyzed
FTA- ABS antigen suspension should be shaken ________,
for how many seconds?
vigorously
10- 15 seconds
What are the possible results for RPR?
reactive
non-reactive
weak
Flocculation is seen in?
RPR test
Fluoresce is seen in?
FTA-ABS
How many drops of the specimen do you place on the card?
1 drop
How many drops of the antigen do you place on the card?
1 drop per specimen
How long do you place the RPR test card on the rotator?
how many rpm?
8 minutes
100rpm
Read the RPR reaction at the end of?
8 minutes
Rotate and tilt card briefly, by hand how many times?
3 - 4 times
Rotate and tilt card helps to differentiate
non-reactive from weakly reactive results
How do you read the RPR Test Card?
MACROSCOPICALLY
Possible results for FTA-ABS are?
reactive or non-reactive
Reactive Rapid Plasma Reagin tests must be confirmed by what?
Fluorescent Treponemal Antibody Absorption test (FTA-ABS)
Mechanical rotator must be calibrated to
100 rpm
Antigen needle must be calibrated to
60 drops
Rapid Plasma Reagin possible results:
reactive, weak, and non-reactive
mononucleosis which is a self-limiting disease caused by
Epstein-Barr virus
Mono is typically seen in
young adults and children
Sx of Mono
- fever
- malaise
- lethargy
- sore throat with exudates
- enlarged lymph nodes on the neck
- mild hepatitis
- enlarged spleen
- blotchy skin rash
mononucleosis causes the accumulation of ________,
causing formation of _______
lymphocytes and monocytes
“heterophile” antibody
Mono mix the antigen for how many secs?
10-15 secs
Mono test the specimen within
24 hours
blood collection for Mono must be stored between ___ to ___ Celsius?
2-8 degrees
Latex reagent for Mono must be shaken how?
gently
For Mono gently rotate the slide for how long?
or how many rpm?
3 minutes
60-100rpm
Positive result for mono
Shows agglutination
Negative result for mono:
smooth, homogenous solution
No Agglutination
Mechanical rotator should be calibrated to how many rpm?
100 rpm
