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Lab > RPR/Monospot > Flashcards

RPR/Monospot Flashcards

1
Q

What is RPR?

A

Rapid plasma reagin

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2
Q

What is a non-treponemal test for serologic detection of the
antibody Reagin?

A

RPR

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3
Q

what is produced in response to an infection of syphilis?

A

antibody Reagin

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4
Q

Syphilis is caused by what?

A

Treponema pallidum

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5
Q

Reagin is found in?

A

serum/plasma

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6
Q

what is the vehicle or “antigen” to see results RPR
macroscopically.

A

Reagin

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7
Q

is a non-treponemal screening test that requires confirmation.

A

Rapid Plasma Reagin

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8
Q

If flocculation is present

A

reactive (positive)

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9
Q

no flocculation is present

A

non-reactive (negative)

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10
Q

What is the confirmatory test for RPR testing?

A

(FTA-ABS)

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11
Q

What does (FTA-ABS) stand for?

A

Fluorescent treponemal antibody-absorbed test

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12
Q

Dead T. pallidum is fixed on a slide with what?

A

patient’s serum

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13
Q

fluoresce =

A

positive

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14
Q

no fluoresce =

A

negative

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15
Q

Which tube color is collected for serum?

A

Red

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16
Q

Which tube color is collected for plasma?

A

Purple

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17
Q

After centrifugation, serum should be __________,
to be an acceptable sample.

A

clear and non-hemolyzed

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18
Q

FTA- ABS antigen suspension should be shaken ________,
for how many seconds?

A

vigorously
10- 15 seconds

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19
Q

What are the possible results for RPR?

A

reactive
non-reactive
weak

20
Q

Flocculation is seen in?

A

RPR test

21
Q

Fluoresce is seen in?

A

FTA-ABS

22
Q

How many drops of the specimen do you place on the card?

A

1 drop

23
Q

How many drops of the antigen do you place on the card?

A

1 drop per specimen

24
Q

How long do you place the RPR test card on the rotator?
how many rpm?

A

8 minutes
100rpm

25
Q

Read the RPR reaction at the end of?

A

8 minutes

26
Q

Rotate and tilt card briefly, by hand how many times?

A

3 - 4 times

27
Q

Rotate and tilt card helps to differentiate

A

non-reactive from weakly reactive results

28
Q

How do you read the RPR Test Card?

A

MACROSCOPICALLY

29
Q

Possible results for FTA-ABS are?

A

reactive or non-reactive

30
Q

Reactive Rapid Plasma Reagin tests must be confirmed by what?

A

Fluorescent Treponemal Antibody Absorption test (FTA-ABS)

31
Q

Mechanical rotator must be calibrated to

A

100 rpm

32
Q

Antigen needle must be calibrated to

A

60 drops

33
Q

Rapid Plasma Reagin possible results:

A

reactive, weak, and non-reactive

34
Q

mononucleosis which is a self-limiting disease caused by

A

Epstein-Barr virus

35
Q

Mono is typically seen in

A

young adults and children

36
Q

Sx of Mono

A
  • fever
  • malaise
  • lethargy
  • sore throat with exudates
  • enlarged lymph nodes on the neck
  • mild hepatitis
  • enlarged spleen
  • blotchy skin rash
37
Q

mononucleosis causes the accumulation of ________,

causing formation of _______

A

lymphocytes and monocytes

“heterophile” antibody

38
Q

Mono mix the antigen for how many secs?

A

10-15 secs

39
Q

Mono test the specimen within

A

24 hours

40
Q

blood collection for Mono must be stored between ___ to ___ Celsius?

A

2-8 degrees

41
Q

Latex reagent for Mono must be shaken how?

A

gently

42
Q

For Mono gently rotate the slide for how long?

or how many rpm?

A

3 minutes

60-100rpm

43
Q

Positive result for mono

A

Shows agglutination

44
Q

Negative result for mono:

A

smooth, homogenous solution

No Agglutination

45
Q

Mechanical rotator should be calibrated to how many rpm?

A

100 rpm

Lab (12 decks)

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