RNA in Translation

Question 1

This form of tRNA only associates with the ribosome.

Answer 1

Peptidyl tRNA

Question 2

This form of tRNA carries amino acids, bound to it’s 3’ end.

Answer 2

Aminoacyl tRNA

Question 3

tRNA is a ________ __________ of RNA, folded into a _____ _______ _______.

Answer 3

Single Strand

Quasi Double Helix

Question 4

At the end of the loop of the RNA strand are:

Answer 4

3 Bases called Anticodon

Question 5

How many possible anticodons and different tRNA’s?

Answer 5

61 tRNA’s

64 Possible Antiocodons

Question 6

How many Anticodons without a tRNA?

Answer 6

3

Question 7

How many possible permeations of anticodon? 4(3) =

Answer 7

64

Question 8

How do amino acids attach to tRNA?

Answer 8

Aminoacyl-RNA synthetase

Question 9

Codon & Anticodon pairings are…

Answer 9

Specific & Complementary!

Question 10

The Genetic Code is the most striking evidence that…

Answer 10

We all evolved from one common ancestor

Question 11

Left side of Genetic Code?

Answer 11

First mRNA Base

Question 12

Right Side?

Answer 12

Third mRNA base

Question 13

Top?

Answer 13

Second mRNA base

Question 14

AUG =

Answer 14

MET or Start! The only codon which carries MET

Question 15

UAA
UGA
UAG

Answer 15

STOP SEQUENCE!

Question 16

The genetic code is somewhat redundant in that…

Answer 16

3rd base is very flexible

Question 17

These are the facilitators for binding & where codon-anticodon interactions occur…

Answer 17

Ribosomes!

Question 18

The ribosomes also catalyze..

Answer 18

Peptide Bonds

Question 19

Two subunits of the ribosome?

Answer 19

Small Subunit

Large Subunit

Question 20

In large there are 3 binding sites for tRNA.

Answer 20

A - aminoacyl tRNA
P - peptidyl tRNA
E - empty tRNA’s
(From left to right EPA, they go from A>P>E)

Question 21

Translation begins when the small subunit…

Answer 21

Binds to the 5’ Cap

Question 22

Close to the 5’ cap will be the..

Answer 22

Start Codon AUG

Question 23

Start codon AUG’s anticodon?

Answer 23

UAC

Question 24

This refers to the start of a protein or polypeptide terminated by an amino acid with a free amine group

Answer 24

Amino Terminus

Question 25

The large subunit will bind…

Answer 25

Aminoacyl-tRNA in P site

Question 26

T/F This is the only time an aminoacyl-tRNA will be here -

Answer 26

TRUE - Then moves to P site and out

Question 27

How can we establish the reading frame?

Answer 27

Start Codon, then every three bases from

Question 28

When does the elongation start?

Answer 28

When new tRNA enters

Question 29

Elongation is noted by the forming of a…

Answer 29

Peptide Bond, added to the carboxy terminal

Question 30

The first tRNA passes it’s amino acid to the:

Answer 30

A site, and then out. A->P, new one in A, pass from P, P out E, etc.

Question 31

How does it make a specific amino acid?

Answer 31

Codon-Anticodon pairings

Question 32

The ribosome shifts in the __’ direction

Answer 32

3’ direction, while mRNA is read 5’-3’

Question 33

Termination happens when the…..

Answer 33

Stop Codon hits the A site

Question 34

Following the stop codon the…

Answer 34

Release Factor enters & hydrolyzes bonds.

Question 35

The hydrolyzing of the bonds releases the:

Answer 35

Peptide & Empty tRNA

Question 36

As the ribosome moves downstream and the peptide becomes longer…

Answer 36

Another ribosome will hop on! Polyribosomes. Teamwork.

Question 37

This process will continue until the tail is completely:

Answer 37

Eaten. Pac-Manned