RLG PARASITES Flashcards

1
Q

pathogen Causing dermatologic lesions

A

Cercopithifilaria
dirofilaria repens

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2
Q

Only sp. That detects antibodies

A

d. immitis

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3
Q

Causes humpsores

A

stephanofilaria

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4
Q

large sp of babesia

A

canis, vogeli

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5
Q

vector of b. vogeli

A

r. sanguineus

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6
Q

vector of b canis

A

dermacentor

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7
Q

small sp. of babesia

A

b. gibsoni

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8
Q

Causes neurologic signs
Adhere and clogs brain capillaries
Highly pathogenic

A

b. bovis

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9
Q

present in ph

A

b vogeli
b. gibsoni
b. bovis
hepatozoon canis
theileria orientalis

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10
Q

vector of t. evansi

A

tabanids

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11
Q

TAE buffer function

A

used as a running buffer in gel electrophoresis to separate nucleic acids.

based on their molecular weight and net charge, the nucleic acids move either slowly or rapidly towards the positive pole. The smaller molecules run faster than the bigger molecules during the gel electrophoresis.

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12
Q

other term for anaplasmosis

A

canine infectious cyclic thrombocytopenia

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13
Q

LAMP temperature

A

60-65C

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14
Q

sample that’s more useful when looking for babesia

A

capillary

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15
Q

stain preferred for examination of protozoan parasites and rickettsiae

A

romanowsky - wright and giemsa

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16
Q

how much blood is needed for blood smear

A

3-5 uL

17
Q

angle for blood smear

A

30-45C

18
Q

fixative for giemsa staining

A

methnaol

19
Q

knott’s concn technique

A

mix 1 ml uncoagulated blood with 9 ml 2% formalin

invert to mix 4x

centrifuge 500g x 5min (2000 rpm)

discard supernatant
staon sediment with 1-2 drops 1% meth blue

add drop of sample and cover

20
Q

No sheath; Body curved
with hooked posterior end

A

acanthocheilonema reconditum

21
Q

Sheathed; Tail is tapered
and with cephalic space.

A

brugia spp.

22
Q

size of brugia

A

170-230 um

23
Q

LAMP can amplify target DNA to what exponent

A

10 raised to 9

24
Q

LAMP utilizes a set of _- primers that recognize a total of six distinct sequences on the target DNA.

A

4-6

25
Q

meaning of CFI

A

color fluorescent indicator

26
Q

composition of CFI

A

hydroxynaphthol blue
gel green

27
Q

LAMP fluorescence is confirmed via machine called

A

FAM filter

28
Q

advantages of microscopic exam

A

accessible
can ID coinfection

29
Q

limitations of microscopic exam

A

low sensitivity
difficult for species lvl ID

30
Q

advantages and disadvantages of serology

A

accessible, high sensitivity
///
Abs take time to develop
cannot distinguish past and present infection
cross-reactivity

31
Q

molecular techniques adv and disadvantages

A

high sensitivity and specificity
multiplex PCR

//

not readily avail
expensive
may take hours
false negative cases

32
Q

LAMP meaning

A

loop-mediated isothermal amplification

33
Q
A