RLG 2 - PCR

Question 1

first step in DNA extraction

Answer 1

collect fresh blood
transfer 200uL to a 1.5 ml microcentrifuge tube

Question 2

2nd step in DNA extraction

Answer 2

add 600ul PB1 mix 1 min

Question 3

3rd step in DNA extraction

Answer 3

add 600 ul PB2 then mix for 10s t

Question 4

if nucleic acid will not be used agad, keep it at _C for _ hour

Answer 4

4C
1HR

Question 5

At the last step, why is it required to stand in RT for 1 minute?

Answer 5

To let DNA bind in inclusion buffer

Question 6

dual dye indicator

Answer 6

CFI (Color Fluorescent Indicator)

Question 7

why negative control should be nuclease free?

Answer 7

nucleases can have the tendecy to actively degrade DNA in samples, that;s why

Question 8

positive color in LAMP

Answer 8

blue

Question 9

negatuve color oin LAMP

Answer 9

purple

Question 10

meaning of TAE

Answer 10

tris-acetate-EDTA

Question 11

Basis on the band size

Answer 11

DNA ladder

Question 12

thermocycler can run _ different conditions simultsaneously, with total of __ samples overall

Answer 12

3
96

Question 13

if the amplicon resolution is 6499 bp what cocnentration of agarose should be used?

Answer 13

1.2

Question 14

band size for anaplasma

Answer 14

500-600

Question 15

band size for b. bovis

Answer 15

356

Question 16

band size for theileria

Answer 16

1500