doc meme 2

Question 1

(barrier and exhange of
gas and liquid molecules)

Answer 1

shell membrane

Question 2

funxn: (respiration and pressure
adjustments)

Answer 2

air sac

Question 3

contains
waste produced by embryo;
Inc. in size with age)

Answer 3

allantoic fluid

Question 4

(source of nourishment for
embryo;

Answer 4

yolk sac

Question 5

dec . in size with age)

Answer 5

yolk sac

Question 6

(protects embryo from physical
damage; exchange system; large in
young embryos)

Answer 6

amnion

Question 7

route of inoculation of virus

Answer 7

chorioallantoic sac route

Question 8

Virus replicates in cells lining the ___ and virus is released
into

Answer 8

chorioallantoic sac
allantoic fluid

Question 9

agent used for enzymatic disaggregation

Answer 9

trypsin-EDTA

Question 10

function of PBS in tissues

Answer 10

prevents the rupture of cells or shriveling of cells due to osmosis and is a non-toxic formulation for the cells

Question 11

what is the optimum size of tissues for mechanical aggregation

Answer 11

0.5-1 mm

Question 12

size of SPL strainer

Answer 12

70um

Question 13

RPM for centrifuge-ing the cells

Answer 13

2000

Question 14

seeding of cells incubation temp and CO2

Answer 14

41C and 5% co2

Question 15

contains all nutrients for the cells to survive

Answer 15

Roswell Park Memorial Institute (RPMI) medium or RPMI 1640 is a form of medium used in cell culture and tissue culture

Question 16

in seeding cells, transfer cell suspension into a __ flask

Answer 16

25cm2 flask

Question 17

qPCR 4 major steps

Answer 17

lysis
binding
washing
elution

Question 18

master mix ingredients

Answer 18

primers, probes, enzymes

Question 19

red cap tube type of control

Answer 19

+

Question 20

blue cap tube type of control

Answer 20

-

Question 21

how many ul is the master mix

Answer 21

980

Question 22

how many ul are the controls

Answer 22

150

Question 23

contains Mastermix except for pathogen
template

Answer 23

• control

Question 24

contains target viral RNA
* checks for correct set-up of the reaction mix

Answer 24

+ control

Question 25

Enables assessment of contamination in the
reaction

Answer 25

neg control

Question 26

checks integrity of sample
* checks for possible PCR inhibition

Answer 26

Internal control (heterologous RNA)

Question 27

positive control CT value

Answer 27

<35

Question 28

negative control CT value

Answer 28

have HEX fluoroscence signal
no FAM or Quasar 670/Cy5 fluorescence signal