MTC Staining of tissues Flashcards
substance that forms a link between the tissue and the stain to allow a staining reaction to take place
mordant
examples of mordants
ammonium and potassium aluminum
example of acidophilic component
cytoplasm
example of basophiluc cellular component
nucleus
action necessary to remove and clear the wax from tissues
deparaffinization
result of incomplete deparaffinization
patches of tissues with unabsorbed dyes
necessary for tissues to absorb stain
hydration
achieved in decreasing strengths of alcohol
hydration
eliminates carrying over of one dye solution to the next.
wash/rinsing
media used for mounting
DPX mountant or albumin+glycerin
most common stain for histo study
H&E
stain color of nucleus
blue
stain color of cytoplasm
pink purplish pink
color of muscle fiber
deep fiber
color of rbcs
orange red
color of calcium
dark blue
color of mucin
grey blue
differential staining
is a technique used to identify different cellular components with different colored dyes.
why are special stains called special
bcoz not used routinely
van gieson stain purpose
differentiate collagen, connective tissue, muscle tissues
agents causing the color red in van gieson
picric acid and acid fuchsin
van gieson color: muscle tissues and blood cells
yellow
Mucins and cartilage stain __
purple
commonly used to ID mast cells, granules, mucins, cartilage
tolouidine blue
metachromatic dye that’s attracted to nucleic acid
tolouidine blue
tolouidine blue staining colors:
nuclei acids:
mucins and cartilage:
blue
purple
mixture of three dytes to diff muscles, collagen fibers, fibrin and erythrocytes in connective tissue.
masson’s trichrome stain
masson’s trichrome staining colors:
nuclei:
cytoplasm, muscle, rbcs:
collagen:
Nuclei- black
Cytoplasm, muscle, erythrocytes- red
Collagen- blue/ green
stains glycogen and ID mucoid substances and fungi
PAS
PAS positive material stain color
magenta pink to red
nuclei color of PAS stain
blue
when does PAS used?
differentiate carcinomas
parasites
