MTC Staining of tissues

Question 1

substance that forms a link between the tissue and the stain to allow a staining reaction to take place

Answer 1

mordant

Question 2

examples of mordants

Answer 2

ammonium and potassium aluminum

Question 3

example of acidophilic component

Answer 3

cytoplasm

Question 4

example of basophiluc cellular component

Answer 4

nucleus

Question 5

action necessary to remove and clear the wax from tissues

Answer 5

deparaffinization

Question 6

result of incomplete deparaffinization

Answer 6

patches of tissues with unabsorbed dyes

Question 7

necessary for tissues to absorb stain

Answer 7

hydration

Question 8

achieved in decreasing strengths of alcohol

Answer 8

hydration

Question 9

eliminates carrying over of one dye solution to the next.

Answer 9

wash/rinsing

Question 10

media used for mounting

Answer 10

DPX mountant or albumin+glycerin

Question 11

most common stain for histo study

Answer 11

H&E

Question 12

stain color of nucleus

Answer 12

blue

Question 13

stain color of cytoplasm

Answer 13

pink purplish pink

Question 14

color of muscle fiber

Answer 14

deep fiber

Question 15

color of rbcs

Answer 15

orange red

Question 16

color of calcium

Answer 16

dark blue

Question 17

color of mucin

Answer 17

grey blue

Question 18

differential staining

Answer 18

is a technique used to identify different cellular components with different colored dyes.

Question 19

why are special stains called special

Answer 19

bcoz not used routinely

Question 20

van gieson stain purpose

Answer 20

differentiate collagen, connective tissue, muscle tissues

Question 21

agents causing the color red in van gieson

Answer 21

picric acid and acid fuchsin

Question 22

van gieson color: muscle tissues and blood cells

Answer 22

yellow

Question 23

Mucins and cartilage stain __

Answer 23

purple

Question 24

commonly used to ID mast cells, granules, mucins, cartilage

Answer 24

tolouidine blue

Question 25

metachromatic dye that’s attracted to nucleic acid

Answer 25

tolouidine blue

Question 26

tolouidine blue staining colors:

nuclei acids:
mucins and cartilage:

Answer 26

blue
purple

Question 27

mixture of three dytes to diff muscles, collagen fibers, fibrin and erythrocytes in connective tissue.

Answer 27

masson’s trichrome stain

Question 28

masson’s trichrome staining colors:

nuclei:

cytoplasm, muscle, rbcs:
collagen:

Answer 28

Nuclei- black
Cytoplasm, muscle, erythrocytes- red
Collagen- blue/ green

Question 29

stains glycogen and ID mucoid substances and fungi

Answer 29

PAS

Question 30

PAS positive material stain color

Answer 30

magenta pink to red

Question 31

nuclei color of PAS stain

Answer 31

blue

Question 32

when does PAS used?

Answer 32

differentiate carcinomas

parasites