qPCR

Question 1

virions vs viruses

Answer 1

virion: infectious form of virus, extracellular, smaller, spheroidal in shape

virus: larger, intracellular, nucleoprotein particle

Question 2

NA extraction steps

Answer 2

lysis
binding
washing
elution

Question 3

after pipeting 20ul of master mix into pcr tubes, the ___, ___, and ___ will be added

Answer 3

negative control first
sample RNA second
positive control last

Question 4

why is there a need to spin?

Answer 4

to remove bubbles

Question 5

primer probe combination

PEDV RNA probe : ____

Answer 5

FAM fluorescence

Question 6

TGEV RNA probe : ___

Answer 6

Quasar® 670/ Cy®5 fluorescence

Question 7

Heterologous RNA control probe = ___

Answer 7

HEX fluorescence

Question 8

PCR uses

Answer 8

medical research
diagnosis
contact tracing
forensics
drug devt

Question 9

how to interpret CT with 4-cycle difference

Answer 9

the pink reaction has 16 times as much target sequence compared to red reaction

Question 10

nuclease-free water why need?

Answer 10

nucleases have the tendency to degrade DNa KASI

Question 11

For tissues, the volume of formalin used should be approximately __ times as that of the tissue.

Answer 11

10

Question 12

formalin can be diluted using

Answer 12

dh20

Question 13

A good transport medium for swabs for viral isolation is

Answer 13

NSS, PBS, 0.85% NaCl if possible with 1% gelatin

Question 14

fecal samples should be chilled at __

Answer 14

4C

Question 15

only sample that needed to be frozen

Answer 15

semen sample

Question 16

use this when bacterial growth must be prevented

Answer 16

bacterial soln

Question 17

refrigeration techniques

Answer 17

ice pack, dry ice

Question 18

chemi preservation techniques

Answer 18

fixing solm
bactericidal soln

Question 19

volt for electrophoresis

Answer 19

5-8V/cm

Question 20

possible cause of false pos in downstream detection test

Answer 20

re-use of equipment
contaminated equipment
contaminated reagents
“ working area

Question 21

possible causes of Failure in
downstream detection tests

Answer 21

bad sample
Damaged reagent bottles or spin columns
incorrect prep
incorrect sample vol

Question 22

possible causes of no flow-through

Answer 22

blood clots
centrifuge force not enough