ELISA Flashcards
1
Q
in ELISA __ is pre-adsorbed in the bottom of the well
A
Ag
2
Q
how much sample is needed in accdg to the protocol
A
100 ul
3
Q
ELISA steps
A
place sample
incubate 1 hr
flick and wash 3x
add 100 ul conjugate
wash 3x
add 100ul substrate and incubate 15mins RT
add 100ul stop solution
4
Q
stop solution function
A
stops the reactions and stabilize the color change
5
Q
OD meaning
A
optical density
6
Q
how many nm is the requirement for checking OD
A
402 nm
7
Q
two main ways of performing ELISA
A
Double antibody technique, to detect antigen
2. Indirect technique, to detect and assay antibody.
8
Q
draw types of ELISA
A
DRAW
9
Q
A
