Review Test 1 Flashcards
Define microbiology.
a specialized area of biology that deals with living things too small to be seen without magnification.
The study of bacteria:
bacteriology
The study of viruses:
virology
The study of fungi:
mycology
The study of algae:
phycology
The study of protozoa:
protozoology
The study of helmiths:
parasitology
How is microbiology used in the health service industry?
physicians and nurses diagnose and treat people with infectious disease
What is immunology?
study the immune chemicals and cells that are produced in response to infection by microorganisms
What us public health/epidemiology?
monitor and control the spread of infectious diseases in the community. WHO CDC
What is food/dairy microbiology?
practical roles of microbes in food or dairy.
What is biotechnical and industrial microbiology?
use microbes metabolism to produce a product such as alcohols or acids
What is genetic engineering/recombinant DNA?
Deliberately alter genetic makeup of a microbe to produce a human hormone or drug vaccine. (human insulin)
What is bioremediation?
the use of microbes to help clean up toxic products in the environment. (euglena mutabilis)
What metric unit is used to measure bacteria?
micrometer
What metric unit is used to measure viruses?
nanometer
What is spontaneous generation?
living things will suddenly appear from nonliving matter
What is biogenesis?
living things arise from other living things of the same kind
Who disproved spontaneous generation?
Louis Pateur
What were Louis Pasteur’s contributions to microbiology?
disproved spontaneous generation
invented pasteurization
created the germ theory of disease
What is germ theory of disease?
a specific microorganism will cause a specific disease. the microrganism is called the caustic agent
Who was Anthony Leeuwenhoek?
Father of bacteriology and protozoology invented microscope 300X did tooth scrapings observed pond water saw animacules
What were Robert Koch’s contributions to microbiology?
Verified the germ theory of disease by finding the caustive agent for anthrax
Purposed Koch’s postulates
Developed lab techniques
What is the caustive agent for anthrax?
Bacillus anthracis
What are Koch’s postulates?
The microbe must be present in every case of the disease but absent from healthy organisms. The suspected microbe must be isolated and grown in a pure culture in the lab. The same disease must result when isolated microbe is innoculated into a healthy host. The same microbe must be isolated again from the diseased host.
What is taxonomy?
a formal system for organizing, classifying, and naming living things.
What is the order of the taxa?
Domain Kingdom Phylum Class Order Genus Species
What contribution did Aristotle make to our taxonomy?
Animalia
Plantae
What contribution to our taxa did Carolus Linnaeus make?
genus
species
What contribution to taxonomy did Ernest Haeckle make?
animalia
plantae
protista
What contribution to taxonomy did Robert Whittaker make?
5 Kingdoms: monera fungi protista animalia plantae
What contribution to taxonomy did Carl Woese and George Fox make?
Three domains: Bacteria Archaea Eukarya studied rRNA
What is the binomial system?
genus
species
Which domains contain prokaryotic organisms?
domain bacteria
domain archaea
Which domain has eukaryotic cells?
domain eukarya
What are the five I’s?
innoculation incubation isolation inspection information gathering identification
What is innoculation?
introduce bacteria into a sterile broth or solid media with the use of a loop or needle
What is incubation?
the placement of an innoculated specimen in a specific temperature to allow growth
What is isolation?
isolate a single colony on a petri plate to obtain a pure culture
What is inspection?
observe the cultures for macroscopic growth of the bacteria, observe microscopically for results of stains
What is information gathering?
testing of cultures for biochemical and enzyme characteristics
What is identification?
Use Bergey’s manual to identify the genus and species of the microorganism.
What is Bergey’s manual used for?
provides keys and charts to aid in identifying a microorganism
What is a pure culture?
one microbe growing in a sample
What is a mixed culture?
more than one microbe growing in a sample
What is a colony?
a cluster of cells growing in a culture medium
What are the three ways to classify culture media?
physical
chemical
functional
What are the physical forms of culture media?
liquid
semisolid
solid
What are the functional types of culture media?
general purpose
enriched media
selective media
differential media
What is general purpose media?
designed to grow a broad spectrum of microbes
What is enriched media?
designed to support the growth of fastidious media (picky)
What is selective media?
designed to allow growth of one type of bacteria while inhibiting others.
What is differential media?
designed to grow several types of organisms but causes their appearance to change.
What is agar?
a complex polysaccharide isolated from the red algaea gelidium
Who’s lab was agar first introduced into and who suggested agar as a possible substitute?
Robert Koch’s lab
Fanny and Walter Hesse
What is the charge of a cell wall?
negative
Negative stains:
use an acidic stain
the stain is repelled from the cell wall
microbe is clear and background is colored
What is an example of a negative stain?
india ink
Positive stains:
use a basic dye
microbe is colored, background is clear
Two types of positive stains:
simple
differential
simple stains:
use a single dye
distinguishes shape arrangement and size
differential stains:
use two different colored dyes
distingushes cell types and cell parts
What is an example of a simple stain?
methelyne blue
crystal violet
What are some examples of differential stains?
gram
acid fast
endospore stains
what is a primary stain?
the first dye you use
what is a counter stain?
the second dye you use
Who is Hans Christian Gram?
he developed the Gram stain
Gram positive cells will stain:
purple
Gram negative cells will stain:
pink
prokaryotic cells:
have no nucleus
one one piece of chromosomal DNA
no organelles
includes all bacteria
eukaryotic cells
have a nucleus
many pieces of chromosomal DNA
organelles
What are the three bacterial shapes?
coccus
bacillus
spiral or curved
what is the atypical shape?
pleomorphic rods
coccus are shaped:
round or oval
bacillus are shaped:
rod shaped
vibrio are shaped:
like a comma
spirillum are shaped:
rigid spiral or helix shape
cells do not bend
amphitricious
spirochette are shaped:
flexible spring shape
endoflagella
what is the shape of pleomorphic rods?
cells vary in size and shape
single coccus:
one round cell
diplococcus:
two round cells
streptococcus:
chain of round cells
staphylococcus:
irregular cluster of round cells
tetrad:
packet of four round cells
sarcina:
cube of eight or sixteen round cells
single bacillus:
one oblong cell
diplobacillus:
two oblong cells
streptobacillus:
chain of oblong cells
palsade:
match sticks or picket fence of oblong cells
what is motile?
bacteria will flip, rotate, or go straight
“run and tumble”
What is nonmotile bacteria?
will only exhibit brownian movement (vibrate)
what protein is found in flagella?
flagellin
atricous:
no flagella
monotricious
one flagella
lophotricious
a tuft of flagella
amphitricious
flagella at both ends
petricious
flagella placed all over the cell
in a straight run which direction does the flagella spin?
flagella spins counterclockwise
in a tumble, which way does the flagella spin?
clockwise
what is periplasmic flagella?
2 or more flagella that lie in the periplasmic space between the cell wall and outer membrane
What is the movement of periplasmic flagella?
corkscrew motion
what are fimbriae?
many short hairs used for attachment to the environment
What are pili?
A single, long, hollow appendage used for attachment to another bacteria during conjugation
what is a postive chemotaxi?
bacteria swims towards attactants like food molecules. Causes fewer tumbles
what is a negative chemotaxis?
bacteria swims away from repellants like poisons
causes more tumbles
What is the composition of the cell envelope?
cell wall
cell membrane
What is the function of the glycocalyx?
to allow bacterial cell to adhere to the environment
Do all cells have a glycocalyx?
no
What is the slime layer of the glycocalyx?
a loose structure that easily washes off