Review Test 1 Flashcards
Define microbiology.
a specialized area of biology that deals with living things too small to be seen without magnification.
The study of bacteria:
bacteriology
The study of viruses:
virology
The study of fungi:
mycology
The study of algae:
phycology
The study of protozoa:
protozoology
The study of helmiths:
parasitology
How is microbiology used in the health service industry?
physicians and nurses diagnose and treat people with infectious disease
What is immunology?
study the immune chemicals and cells that are produced in response to infection by microorganisms
What us public health/epidemiology?
monitor and control the spread of infectious diseases in the community. WHO CDC
What is food/dairy microbiology?
practical roles of microbes in food or dairy.
What is biotechnical and industrial microbiology?
use microbes metabolism to produce a product such as alcohols or acids
What is genetic engineering/recombinant DNA?
Deliberately alter genetic makeup of a microbe to produce a human hormone or drug vaccine. (human insulin)
What is bioremediation?
the use of microbes to help clean up toxic products in the environment. (euglena mutabilis)
What metric unit is used to measure bacteria?
micrometer
What metric unit is used to measure viruses?
nanometer
What is spontaneous generation?
living things will suddenly appear from nonliving matter
What is biogenesis?
living things arise from other living things of the same kind
Who disproved spontaneous generation?
Louis Pateur
What were Louis Pasteur’s contributions to microbiology?
disproved spontaneous generation
invented pasteurization
created the germ theory of disease
What is germ theory of disease?
a specific microorganism will cause a specific disease. the microrganism is called the caustic agent
Who was Anthony Leeuwenhoek?
Father of bacteriology and protozoology invented microscope 300X did tooth scrapings observed pond water saw animacules
What were Robert Koch’s contributions to microbiology?
Verified the germ theory of disease by finding the caustive agent for anthrax
Purposed Koch’s postulates
Developed lab techniques
What is the caustive agent for anthrax?
Bacillus anthracis
What are Koch’s postulates?
The microbe must be present in every case of the disease but absent from healthy organisms. The suspected microbe must be isolated and grown in a pure culture in the lab. The same disease must result when isolated microbe is innoculated into a healthy host. The same microbe must be isolated again from the diseased host.
What is taxonomy?
a formal system for organizing, classifying, and naming living things.
What is the order of the taxa?
Domain Kingdom Phylum Class Order Genus Species
What contribution did Aristotle make to our taxonomy?
Animalia
Plantae
What contribution to our taxa did Carolus Linnaeus make?
genus
species
What contribution to taxonomy did Ernest Haeckle make?
animalia
plantae
protista
What contribution to taxonomy did Robert Whittaker make?
5 Kingdoms: monera fungi protista animalia plantae
What contribution to taxonomy did Carl Woese and George Fox make?
Three domains: Bacteria Archaea Eukarya studied rRNA
What is the binomial system?
genus
species
Which domains contain prokaryotic organisms?
domain bacteria
domain archaea
Which domain has eukaryotic cells?
domain eukarya
What are the five I’s?
innoculation incubation isolation inspection information gathering identification
What is innoculation?
introduce bacteria into a sterile broth or solid media with the use of a loop or needle
What is incubation?
the placement of an innoculated specimen in a specific temperature to allow growth
What is isolation?
isolate a single colony on a petri plate to obtain a pure culture
What is inspection?
observe the cultures for macroscopic growth of the bacteria, observe microscopically for results of stains
What is information gathering?
testing of cultures for biochemical and enzyme characteristics
What is identification?
Use Bergey’s manual to identify the genus and species of the microorganism.
What is Bergey’s manual used for?
provides keys and charts to aid in identifying a microorganism
What is a pure culture?
one microbe growing in a sample
What is a mixed culture?
more than one microbe growing in a sample
What is a colony?
a cluster of cells growing in a culture medium
What are the three ways to classify culture media?
physical
chemical
functional
What are the physical forms of culture media?
liquid
semisolid
solid
What are the functional types of culture media?
general purpose
enriched media
selective media
differential media
What is general purpose media?
designed to grow a broad spectrum of microbes
What is enriched media?
designed to support the growth of fastidious media (picky)
What is selective media?
designed to allow growth of one type of bacteria while inhibiting others.
What is differential media?
designed to grow several types of organisms but causes their appearance to change.
What is agar?
a complex polysaccharide isolated from the red algaea gelidium
Who’s lab was agar first introduced into and who suggested agar as a possible substitute?
Robert Koch’s lab
Fanny and Walter Hesse
What is the charge of a cell wall?
negative
Negative stains:
use an acidic stain
the stain is repelled from the cell wall
microbe is clear and background is colored
What is an example of a negative stain?
india ink
Positive stains:
use a basic dye
microbe is colored, background is clear
Two types of positive stains:
simple
differential
simple stains:
use a single dye
distinguishes shape arrangement and size
differential stains:
use two different colored dyes
distingushes cell types and cell parts
What is an example of a simple stain?
methelyne blue
crystal violet
What are some examples of differential stains?
gram
acid fast
endospore stains
what is a primary stain?
the first dye you use
what is a counter stain?
the second dye you use
Who is Hans Christian Gram?
he developed the Gram stain
Gram positive cells will stain:
purple
Gram negative cells will stain:
pink
prokaryotic cells:
have no nucleus
one one piece of chromosomal DNA
no organelles
includes all bacteria
eukaryotic cells
have a nucleus
many pieces of chromosomal DNA
organelles
What are the three bacterial shapes?
coccus
bacillus
spiral or curved
what is the atypical shape?
pleomorphic rods
coccus are shaped:
round or oval
bacillus are shaped:
rod shaped
vibrio are shaped:
like a comma
spirillum are shaped:
rigid spiral or helix shape
cells do not bend
amphitricious
spirochette are shaped:
flexible spring shape
endoflagella
what is the shape of pleomorphic rods?
cells vary in size and shape
single coccus:
one round cell
diplococcus:
two round cells
streptococcus:
chain of round cells
staphylococcus:
irregular cluster of round cells
tetrad:
packet of four round cells
sarcina:
cube of eight or sixteen round cells
single bacillus:
one oblong cell
diplobacillus:
two oblong cells
streptobacillus:
chain of oblong cells
palsade:
match sticks or picket fence of oblong cells
what is motile?
bacteria will flip, rotate, or go straight
“run and tumble”
What is nonmotile bacteria?
will only exhibit brownian movement (vibrate)
what protein is found in flagella?
flagellin
atricous:
no flagella
monotricious
one flagella
lophotricious
a tuft of flagella
amphitricious
flagella at both ends
petricious
flagella placed all over the cell
in a straight run which direction does the flagella spin?
flagella spins counterclockwise
in a tumble, which way does the flagella spin?
clockwise
what is periplasmic flagella?
2 or more flagella that lie in the periplasmic space between the cell wall and outer membrane
What is the movement of periplasmic flagella?
corkscrew motion
what are fimbriae?
many short hairs used for attachment to the environment
What are pili?
A single, long, hollow appendage used for attachment to another bacteria during conjugation
what is a postive chemotaxi?
bacteria swims towards attactants like food molecules. Causes fewer tumbles
what is a negative chemotaxis?
bacteria swims away from repellants like poisons
causes more tumbles
What is the composition of the cell envelope?
cell wall
cell membrane
What is the function of the glycocalyx?
to allow bacterial cell to adhere to the environment
Do all cells have a glycocalyx?
no
What is the slime layer of the glycocalyx?
a loose structure that easily washes off
What is the capsule layer of the glycocalyx?
firmer structure that is bonded to the cell
what is the function of the cell wall?
determines shape and prevents lysis due to changing osmotic pressures
All bacteria have a cell wall except:
mycoplasma
What is the major molecule in the cell wall?
peptidoglycan
What is the structure of the cell wall in gram positive bacteria?
thick peptidoglycan layer
teichoic acid
no outer membrane
What is the structure of the cell wall in gram negative bacteria
thin peptidoglycan layer
no teichoic acid
has an outer membrane external to the cell wall
What composes the cell membrane?
phospholipid bilayer embedded with proteins
what is the function of the cell membrane?
decides what goes in and comes out of the cell.
serves as DNA anchor during bionary fusion
contains enzymes for cell wall synthesis
forms mesosomes
anchors bacterial flagella
what are mesosomes?
finger-like pockets in the cytoplasm that increase surface area
Do all bacterial cells have a cell membrane?
yes
what is cytoplasm?
dense, gelatinous solution of sugars, amino acids and salts
where do all chemical reactions occur?
cytoplasm
what is a chromosome?
single, circular, double-stranded DNA molecule that contains all of the genetic information required by the cell
what is a plasmid?
free small circular double stranded DNA
very important to bacterial resistance
used in genetic engineering
how do prokaryotic ribosomes differ from eukaryotic?
prokaryotic are smaller
what is the size of prokaryotic ribosomes?
70S =50S +30S
what is the size of a eukaryotic ribosome?
80S= 60S + 40S
what is poly B hydroxybutyrate
carbohydrate store
what is polysaccharide granules
starch or glycogen store
what is metachromatic granules?
phosphate stores
what is magnetosome?
iron stores
what are gas vesicles?
various gases for bouyancy
What are inclusions and granules?
intracellular storage bodies. the cell can use them when the environmental sources are depleted
What are endospores?
inert resting cells produced by some G+ genera
what species produces endospores?
bacillus and clostridium
what environment would cause sporulation?
extrreme environments where nutrients are scarce
what environment would cause germination
one where nutrients are abundant
What are the six factors that effect microbes existence whether in the labratory or in their habitat:
nutrients osmotic pressure temperature pH gas other organisms or microbes
what is nutrition?
a process by which nutrients are aquired from the environment and used in cellular activites such as metabolism and growth
what is a macronutrient?
they are required in large quantities and play principal roles in cell structure and metabolism
what is a micronutrient?
they are required in smaller amounts and are needed for enzyme function and maintenence of protein structure
what are some examples of a macronutrient?
carbon hydrogen oxygen nitrogen phosphorus sulfer CHNOPS
what are some examples of micronutrients?
calcium sodium potassium zinc copper nickel
photoautotroph:
carbon source: CO2
energy source: sunlight
Examples of photoautotroph:
cyanobacteria
sulfer bacteria
algae
plants
chemoautotroph
carbon source: C02
energy source: inorganic
examples of chemoautotroph
methanogens
photoheterotroph:
carbon source: organic
energy source: sunlight
examples of photoheterotroph:
green and purple photosynthetic bacteria
chemoheterotroph:
carbon source: organic
energy source: organic
examples of chemoheterotroph:
fungi
protozoa
many bacteria
animals
what are the two types of chemoheterotroph?
saprobes
symbiotic
what are saprobes:
free-living microbes that get organic recycled material from dead organisms
what are symbiotic chemoheterotrophs:
obtian organic material from living organisms
diffusion:
the net movement of solute molecules from their area of higher concentration to lower concentration
osmosis:
the net movement of water molecules from their area of higher concentration to lower concentration
facilitated difusion:
the movement of larger molecules from an area of higher oncentration to lower concentration with the use of a transport protein
isotonic:
the environment is equal in concentration to the cell’s internal environment. No net movement of water into or out of the cell
hypotonic:
the solute concentration of the external environment is lower than the cell’s internal environment; net movement of water into the cell
hypertonic
the environment has a higher solute concentration that the cytoplasm; net movement of water out of the cell
endocytosis:
things move inside the cell
exocytosis:
things move out of the cell
2 types: phagocytosis and pinocytosis
pinocytosis
cell drinking
phagocytosis
cell eating
How have organisms adapted to living in hypotonic environments?
amobeas and paramecium have contractile vacuoles that constantly fill with water and expel it to the exterior of the cell
How have organisms adapted to living in hypertonic environments?
they increase the solute concentration by storing salt
What kind of environment do most bacteria live in?
hypotonic
What would happen to a bacterial cell living in a hypertonic environment?
lysis
What is optimum growth temperature?
promotes the fastest rate of growth and environment
halophiles:
live in high salt environments
psychrophiles
live in -15C to 20C
mesophiles
live in 10C to 50C
thermophiles
live in 45C to 80C
hyperthermophiles
live in 67C to 105C up to 250C
neutrophiles
pH 6-8
acidophiles
pH 0-2
alkalinophiles
pH 8-11
mutualistic:
both members benefit; normal flora
commensalism:
one member benefits, the other is not harmed; nomal flora
parasitic:
one member benefits, the host is harmed; pathogens
synergism:
members cooperate and share nutrients
antagonism:
some members are inhibited or destroyed by others
what are symbiotic relationships:
mutualistic
commensalism
parasitic
what are nonsymbiotic relationships?
synergism
antagonism
what is generation time?
period of time for one cell to become two cells
what are the four phases of the bacterial growth curve?
lag phase
exponential phase
stationary phase
death phase
lag phase
no rise in number of cells
exponential phase
cells divide at maximum rate
stationary phase
growth equals death
death phase
cell death is greater than cell growth
Bacteria reproduce only byasexual reproduction called
bionary fission
what is bionary fission?
a. early phase of cycle
b. parent cell enlarges, cell wall notch develops that becomes septum, chromosome attaches to the cell membrane and replicates
c. septum grows inward and chromosomes are pulled toward opposite ends of the cell. cell enlarges
d. septum synthesized completely through cell center
e. two seperate daughter cells
aerobe:
must have oxygen for growth
microaerophile
requires less oxygen that is found in air
facilitative anaerobe
grows with or without oxygen
strict anaerobe
does not use oxygen for growth; dies in presence of oxygen
Is Staphylococcus auerus gram positive or negative?
gram positive
what is the arrangement of staphylococcus aureus
clusters
What toxins are found in staphlyococcus aeurus?
entertoxins
exfoliative toxin
toxic shock toxin
what is the transmission of staphylococcus aureus?
it can invade through skin wounds, follicles, skin glands
what diseases come from staphylococcus aureus?
staphylococcal food poisoning
staphylococcal scalded skin syndrome
toxic shock syndrome
what vaccines are used for staphylococcus aureus?
staphvax
How do you treat staphylococccus?
susceptibility test
what does entertoxin damage?
intestinal lining (food poisoning)
what doese exfoliative toxin damage?
seperates the epidermal layer from the dermis (SSSS)
what does toxic shock toxin damage?
several organs
what is folliculitis?
a mild infection of hair follicles or glands (pimples) (staph)
what is a boil or furnacle?
infection of a single follicle progresses into a large, red, tender abscess or pustule (staph)
what is a carbuncle?
cluster of furnucles usually found on the back of the neck (staph)
what is impetigo?
infection of the skin with bubble like epidermal swellings. most common in newborns (staph)
what is a wound?
any potential cut, scrape or bug bite
what is staphylococcal food poisoning?
food intoxication
aquired by contaminated food
what is staphylococcal scalded skin syndrome?
a disease aquired in a newborn by infection of umbilical stump or eyes.
When was toxic shock syndrome first identified?
1978 in women using tampons
what are some special characteristics of streptococcus?
facultative anaerobes do not form catalase requires enriched media small non-pigmented colonies sensitive to drying, heat and disinfectants
is streptococcus gram positive or negative?
gram positive
What is the arrangement of streptococcus?
in pairs or chains of varying length
What is group A streptococcus?
streptococcus pyogenes
What is the transmissson of streptococcus pyrogenes?
contact, droplets food or fomites
What are some localized diseases caused by streptolococcal pyrogenes?
pyoderma
erysipelas
necrotizing fasciitis
streptococcal pharyngitis
What is pyoderma?
streptococcal impetigo
highly contagious rash aquired by contact with blisters or crusts (contaminated fingers)
What is erysipelas?
highly contagious rash that enters through a break in the skin
what is necrotizing fasciitis?
flash eating disease aquired by a cut, scrape or insect bite. Effects connective tissue of the dermis
what is streptococcal pharyngytis?
strep throat
aquired by droplets
what are some systemic diseases caused by streptolococcal pyrogenes?
scarlet fever
pneumonia
toxic shock syndrome
what is scarlet fever?
occurs after strep throat due to pyrogenic toxin or erythrogenic toxin. bright red rash that lasts ten days
what is pneumonia?
caused by strep usually a secondary infection to influenza or other respiratory disease (strep)
what is toxic shock syndrome (strep)?
more severe than S. aureus TSS
what are some long term complications of streptococcus pyrogenes?
rheumatic fever
acute glomerulonephritis
what is rheumatic fever?
inflammatory condition of joints, heart and subcutaneous tissues. due to cross reacting antibodies that attack connective tissue (follows strep throat) (strep)
what is acute glomerulonephritis?
disease of kidney and tubular epithelia
due to immune complex deposittion in the kidney
what is treatment for streptococcus pyrogenes?
penecillin V
vaccine in the works
what is streptococcus pneumoniae?
major virulence factor: capsule
most people carry it as normal flora
affects people living in close quarters
aquired by droplets or endogenous
what is pneumonia?
occurs when cells are aspirated into the lungs of susceptible individuals
pneumococci multiply and induce an overwhelming inflammatory response
s. pneumoniae s. pyrogenes
what is otitis media?
middle ear infections in children s. pneumonia
what is bacterial meningitis?
bacteria progress via bloodstream via CSF. Inflames the meninges, excess CSF creates pressure on the brain and spinal cord. permanent damage or death may occur s. pneumoniae
what is the treatment for streptococcus pneumoniae?
penicillin G or V
what vaccines are used for streptococcus pneumoniae?
pneumovax
prevnar
What group is streptococcus pneumoniae?
group A
What group is streptococcus agalactiae?
group B
what is streptococcus agalactiae?
regulary resides in vagina, pharynx and large intestine
How are pregnant women treated for streptococcus agalactiae?
screened during 3rd trimester; treated with IV antibiotics
How is steptococcus agalactiae treated?
penicillin
What bacteria belongs in the viridians group?
streptococcus mutans
streptococcus salivarus
What are streptococcus mutans and streptococcus salivarus?
bacteria that affects the gums and teeth.
How do you treat streptococcus mutans and streptococcus salivarus?
persons with preexisting heart conditions should receive antibiotics before dental surgeries
what diseases are caused by streptococcus salivarus and streptococcus mutans?
dental caries
endocarditis
What is enterococcus faecalis?
normal colonist of the large intestine; causes nosocomial infections
what is the treatment of enterococcus?
combo of drugs: VRE vancomycin resistant enterococcus
What are some characteristics of Family neisseriaceae?
gram negative
bean shaped diplococci
resides in mucous membrane
what are the two pathogens in family neisseriaceae?
neisseria gonorrhoeae
neisseria meningitidis
what are some characteristics of genus neisseria?
gram negative bean shaped diplococci
capsules on pathogens
aerobic or microaerobic
produce catalase and cytochrome oxidase
neisseria gonorrheae causes
genital gonorrhea in males
genitourinary gonorrhea in female
extragenital
gonococcal eye infections in newborn
what are some characteristics of genital gonorrhea in males?
painful urination
scarring and infertility possible
10% are asymptomatic
what are some characteristics of genitourinary gonorrhea in women?
painful urination, discharge
50% of women are asymptomatic
can continue to pelvic inflammatory disease
what are some characteristics of extragenital gonorrhea?
different sexual practices can cause infections outside of the reproductive system (throat/anus)
what is gynococcal eye infections of the newborn?
born to infected mother
can cause blindness
treated with silver nitrate or antibiotics
how do you treat neisseriae gonorrhea?
penicillin
tetracycline
Neisseria meningitidis causes
meningitis
How is meningitis aquired?
droplets; usually people living in close quarters
How does menngitis begin?
bacteria enters the bloodstream, crosses the blood-brain barrier, permeates the meninges and grows in the CSF
What is meningcoccemia?
serious complecation that occurs when endotoxin causes hemorrhage and shock. Can be fatal
What is the treatment for meningococcemia?
penicillin
what are the two most common causes for meningitis?
steptococcus pneumoniae
nesseria meningitidis
Louis jablot:
believed even microbes have parents
Francesco Redi
spontaneous generation (meat in a jar)
VBNC
viable but nonculturable
biofilms
microbes cling together in complex masses
What binds biofilms?
glycocalyx
Biofilms are sometimes
impervious to disinfectants and antibiotics
Culture Club/Boy George
I’ll tumble for ya
“run and tumble”
Queen and David Bowie
“Under pressure”
Osmotic Pressure
Snow Patrol
Chasing Cars
psychrophiles
PPNG:
pennicillinase producing neisseriae gonorrheae
TRNG
tetracycline producing neisseria gonorrhea