respiration - lecture 4 Flashcards
describe transport of o2
amount of gas dissolved in liquid is proportional to its partial pressure - henrys law increase partial pressure in air = more diffuses into fluid
proportional to pressure gradient
describe o2 physically dissolved in plasma
o2 is relatively insoluble in h2o
amount of o2 dissolved in blood = v small
linearly proportional to po2
ex = in 100ml plasma, 0.3ml of o2 at a po2 of 100mmhg - that will dissolve
if o2 were only found in plasma what would happen
the tissue demand for o2 would never be met
o2 consumption (VO2) by body cells = 300ml o2/min
describe Hb
found in rbcs
permits whole blood to take up 65 times as much o2 as plasma at po2 100mmhg
what does each molecule of Hb have
4 subunits bound together
each made up of heme joined to a globin
heme contains fe++ ion that can bind to one molecule o2
= 4 oxygens per hb
what is Hb essential for
transport of o2 by blood since combines rapidly and reversibly with o2
Hb + O2 <–> HbO2
give ex of how Hb helps
at po2 100mmhg
total amount o2 dissolved in blood = 0.3vol. %
total amount of O2 bound to Hb = 19.5 vol.% (65x more)
the total amount of o2 in arterial blood = 20vol%
does o2 bound to hb contribute to po2 of blood, explains
NOOO
(like new compound, not in plasma anymore)
only molecules dissolved in blood plasma contribute to po2
the po2 of the plasma does determine amount of o2 that combines with Hb = higher po2 in plasma = more o2 binds Hb
describe HbO2 dissociation curve
determines amount of O2 carried by Hb for any given partial pressure
curve flat at high values po2 and steep at low values po2
sigmoidal shape
what does Hb provide
automatic mechanism that matches tissues o2 supply to tissue need
describe at low values of po2 - o2 dissociation curve
as seen in peripheral tissues
small drop in po2 unloads the o2 from the hb to the tissue
give ex of drop in po2 - o2 dissociation curve
drop in po2 from 40–>20mmhg results in decrease in %HbO2 from 75–>35%
drop in po2 from 100–>80mmhg results in decrease of less than 3%
describe po2 - o2 dissociation curve graph
40–>20, 75% so gives up o2 so tissue can use it
at steep portion = decrease Hb sat at 38%, desat so lets O2 go to exercising muscles
100–>80, at alveolar level want to saturate so can transfer to peripheral, desat not by much even tho still 20mmhg increase
describe climbing mount everest
barometric pressure is less than at sea level bc at high altitude
less pressure of air on shoulders
might be 80 still want to sat hB - do not want to desat = tissues need it
describe o2 dissociation curve o2 - total
total 02 = sum of o2 dissolved and combined with Hb
even at po2 600 - dissolved o2 = o2 dissolved in plasma is v small
describe pathway of o2
alveolar level - diffuses down pressure gradient - dissolved o2 then binds to hb - in pulmonary cap
goes to heart
o2 moves down pressure gradient and moves out of rbc and diffuses out towards cells
describe shape of hemoglobin dissociation curve
the quaternary structure of hb determines affinity for o2
combination of first heme of hb with o2 increases affinity of second heme for o2, etc. = cooperative binding
conformational change as o2 binds hb
describe shape of myoglobin dissociation curve
hyperbolic shape
desat only at very low partial pressures
safety mechanism = still have reserve of o2 at skeletal muscle level