Regs Flashcards
Dry bulb temp for Mouse, rat, hamster, gerbil, GP
20-26 C
68-79 F
Cat, dog, NHP
18-29 C
64-84 F
Dry bulb temp for Farm animals, poultry
16-27 C
61-81 F
Dry bulb temp for rabbit
16-22 C
61-72 F
IACUC requirements for PHS policy
Not less than five members
1) DVM
2) scientist
3) non-scientist
4) non-affiliated member
What type of diet is suitable for studies conducted in compliance with FDA Good Laboratory Practice standards?
Certified
What is the maximum number of voting IACUC members that can be associated with a single administrative unit?
3
Who is exempt from USDA license?
- Retail pet stores that sell “nondangerous” pets
- Sell non-wild or non-exotic animals and makes <$500 gross
- Anyone who has ≤4 bitches/queens and sells only the offspring.
- Anyone who sells <25 dogs and/or cats per year for research, which were born and raised on his/her property. (May obtain voluntary license – Class A)
- Anyone who transports, buys, sells or breeds for food, fiber, or personal enjoyment.
- Anyone who breeds domestic pets for direct retail sales for the buyer’s own use, except dogs used for hunting, breeding, or security.
Carbon monoxide should not exceed __ ppm according to Ag Guide.
150 ppm
Ammonia should not exceed __ ppm according to Ag Guide.
25 ppm
Hydrogen sulfide should not exceed __ ppm according to Ag Guide.
50 ppm
Methane should not exceed __ ppm according to Ag Guide.
50,000 ppm
OSHA permissible dust levels over 8-hour day
5 mg/m3 for respirable dust (smaller particles that can enter lungs)
15 mg/m3 for total dust (includes larger particles that may be inhaled but not enter lungs)
According to the Animal Welfare Act and its regulations, official identification tag numbers used for cats and dogs may not be used more than once within what time period?
5 years
According to AWA, fence for outdoor NHP enclosure must be ___ feet tall and ___ feet from enclosure.
6 ft high and must be at least 3 ft from primary enclosure
What should be included in a USDA certificate of a random-sourced dog obtained by a research facility from a dealer?
Description of dog, where dog/cat came from, dealer’s info, research facility’s info
What would be exempt from USDA registration?
Federal government, elementary/secondary school labs, ag research
How long should dealer, exhibitor, broker, operator of an auction sale, carrier, or intermediate handler keep records for?
1 year
According to the Animal Welfare Act and it regulations, what is the minimum frequency for sanitizing rabbit primary enclosures?
30 days
Exceptions for 1 NHP per transport enclosure
Mother-infant
Established male-female
Compatible juvenile pair
For dogs and cats Ambient temp must not be <__ or >__ for more than 4 hours. If the temperature is >__, must provide auxiliary ventilation.
45
85
85
CO2 displacement rate for rodents per AVMA Euth Guidelines
30-70% volume per minute
Cervical dislocation is AC for mice and rats < ___ grams and rabbits < ___ kg
200 g
1 kg
Need to report any issues with nucleic acid work within ___ days to NIH OSP and IBC.
30
Institutional Biosafety Committee (IBC) must have no fewer than ___ members. At least ___ members must not be affiliated with the institution and must include at least ___ member representing the laboratory technical staff.
5
2
1
When do you need to appoint a biosafety officer?
- Large volume (> 10 liters of culture)
- BL3 or BL4 nucleic acid work
- Any gene drive organisms
Which group sets containment levels for nucleic acid work?
IBC (Institutional Biosafety Committee)
