Birds Flashcards
Class for birds
Class: Aves
Domestic chicken genus species
Gallus domesticus
White leghorn most common research chicken
What chicken is model of vitiligo
Smyth Line (SL)
Which chicken is model of Herpesvirus-Induced Atherosclerosis?
White Leghorn
Induced by Marek’s disease herpesvirus
Which chicken is a model of scleroderma (systemic sclerosis)?
White Leghorn
Which chicken is a model of Hashimoto’s Thyroiditis? What two rodents?
Spontaneous, hereditary autoimmune thyroiditis (SAT) of Obese Strain (OS) Chicken
Buffalo rat and Multimammate Rats (Mastomys)
What chicken is a model for Hereditary Muscular Dystrophy? What is inheritance pattern?
New Hampshire chicken
Autosomal recessive
What is the chicken alternative to the Draize eye irritation test in rabbits? What else can it be used for?
Chorioallantoic membrane (CAM) test
Implantation studies (immunologically privileged)
Genus species of Japanese quail
Coturnix japonica
Coturnix japonica use in research
Model for embryogenesis
Birth defects, hypercholesterolemia and xanthomatosis, glycogen storage disease
Pompe’s dz (myotonic dystrophy)
Neurofilament-deficient mutant (“Quiver”)
Quail hypothalamic systems exhibit neuroplasticity – responsive to testosterone
Cloacal gland is androgen responsive, similar to the prostate
Osteoporosis model, as female birds use bones are mineral reservoir for egg production
Acceptable euthanasia for poultry
Barbiturates and Barburative Acid Derivatives (IV)
Acceptable with conditions euthanasia for poultry
CO2 (most common)
CO (at least 6%)
N2 or Ar
Cervical dislocation (but turkeys and broiler breeds too large)
Decapitation
Manual blunt force trauma
Electrocution
Gunshot
Adjunctive euthanasia methods for poultry
KCl or magnesium sulfate (IV or IC, must be unconscious)
Exsanguination (must be unconscious)
Euthanasia for embryonated eggs of poultry
CO2 (20 min, may require 80-90%)
Cooling (4 hours at 40F)
Freezing
Addling
Maceration (less than 72 hours old)
Acceptable euthanasia methods for avians (eg research)
- Barbiturates and BADs (IV preferred or intracoelomic; intracardiac or IO if unconscious)
Acceptable with conditions for avians (e.g. research)
- Inhaled anesthetics
- CO2 (>40% for diving, 80-90% for pips and newly hatched)
- CO, N2, Ar
- Cervical dislocation (<200g, last resort)
- Decapitation (<200g)
- Gunshot (only if cannot restrain)
Adjunctive euthanasia methods for avians (eg research)
- KCl (IV or IC in unconscious)
- Exsanguination (unconscious)
- Thoracic compression (insentient)
Unacceptable euthanasia for avians (eg research)
Thoracic compression (if not deeply anesthetized/insentient)
Euthanasia avian eggs <80% vs >80% incubation
<80%
1. CO2 > 20 min
2. Cooling (4h at 4C) **
3. Freezing**
4. Addling
> 80%
1. CO2 > 20 min
2. Decapitation
3. Anesthetic overdose
4. Addling
Which birds require gravel/grit in diet?
Those that eat whole seeds
Pigeon (Columba livia) and dove
What is produced by male Japanese Quail after puberty?
Foam from the cloacal gland
Deposited during natural copulation can be retained by the female for more than 12h and contributes to prolonged sperm motility
Can also be due to presence of bacteria in cloacal gland
Can palpate cloacal gland and see this and know it is a male
What virus in Japanese quail causes large basophilic INIB
Necrotizing proliferative bronchitis; and hepatic, splenic, and cloacal bursa necrosis
Quail Bronchitis Virus (QBV, type 1 avian adenovirus)
3 most common viruses in Japanese quail
quail bronchitis virus/QBV (Adenoviridae), Newcastle virus (Paramyxoviridae),
and Eastern Equine Encephalomyelitis (Genus Alphavirus, Togaviridae)
How is EEE spread to Japanese Quail?
mosquito,
Culisetta melanura
What causes the fatal disease Ulcerative enteritis ‘quail disease’? What tissues other than GI are affected? How to dx?
Clostridium colinum
Liver/spleen
Dx: Fresh liver culture on tryptose agar
Japanese quail can be infected by which two serovars of Salmonella enterica
pullorum
disease (Salmonella pullorum) and fowl typhoid
(Salmonella gallinarum)
What family contains Japanese quail?
Phasianidae
What family contains zebra finch?
Estrildidae
Zebra finch use in research
Neurobiology - similar striatal and pallidal cell types to mammalian brain
Songbird vocal learning of human speech
What is special about zebra finch digits?
Feet are anisodactylous: 3 digits pointing forward, one back
Do birds generally have more lymphocytes or granulocytes?
Lymphocytes
(main granulocyte = heterophil)
What species of Mycobacteria affects psittacines/passerine birds vs other birds
M. avium MC agent in birds with mycobacteriosis, in psittacines/passerines actually M genavense MC
Ddx coelomic distention female zebra finch
Egg-binding (if see yellow, yolk coelomitis –> euthanize)
Ovarian tumor
Ddx zebra finch periocular swelling, SQ nodules
Polyomavirus
Mites in zebra finches
Dermanyssus gallinae, Ornithonyssus sylviarum are blood sucking mites
Skin mites: Backericheyla and Neochyletiella
SCALY mite: Knemidocoptes pilae (beak and feet)
Air sac mite: Sternostoma tracheacolum
Feather mite: Megninialges sp.
T/F In captivity, C. japonica and C. coturnix (European common quail) will interbreed and produce fertile hybrids.
True
Incubation time for chickens vs quail
Quail devo faster than chickens (16 vs 21 days)
How to sex C. japonica? And at what age do they exhibit sexual dimorphism/reach sexual maturity?
Females larger
6-8 weeks
C. japonica and G. domesticus have a diploid number of ___ chromosomes.
78
What is used to isolate DNA from C. japonica? What is used to screen for phenotype?
chorioallantoic membrane (CAM)
observe hatchlings for feather color or developmental abnormalities
For C. japonica what are 3 tissues/cells to use to determine transgene expression using fluorescent microscopy?
Fluorscent microscopy using: 1) Feather blood cells, 2) shell (eggshell) membrane cells or 3) CAM (chorioallantoic membrane)
High/low temp is concern for shipping quail eggs. Upon arrival store at ___C. Embryonic death occurs at temps less than ___C.
High temp can compromise fertility
Store at 13C (~55F) with open tray of water for humidity.
4C (maybe bc water freezes?)
What is in order
Galliformes?
chicken, turkeys, quail
What is in order Anseriformes?
ducks, geese, swans
What is in order Columbiformes?
Pigeons, doves
What is in order Psittaformes?
Parrots
What is in order Passiformes?
crows, sparrows, finches
What is the optimum temperature for egg production?
50-70C
Ddx for air sacculitis?
Mycoplasma, Pasteurella, E. coli, Hemophilus
You note increase mortality in chickens ranging from 14-16wks old. Wet gangrenous lesion can be seen in the skin of the wings and legs. What are the most likely primary and secondary agents involved respectively?
Infectious Bursal disease (Birnaviridae; causes immunosuppression); S. aureus & C. septicum (cause of malignant edema in ruminants)
Gallapova meleagridis with Cutaneous Infarction and Vascular Congestion (appears purple)
(Turkey)
Erysipelothrix rhusiopathiae
What is the most appropriate combination of floor space and environmental temperature to house Japanese quail?
0.25 ft2/animal; 23°C (22-25)
How is housing different for quail and zebra finches?
Quail are ground-dwelling - do not need elevated perches like zebra finches
What is dubbing in chickens?
Partial removal of comb
Where not to inject euthanasia solution in birds?
Air sacs, humerus, and femur - will drown them
Which avian species WILL NOT eat in low light or darkness, thus requiring a restriction on these time periods due to welfare concerns?
Gallus domesticus (chicken)
What lesion is observed in the chicken eye with Marek’s disease (alphaherpesvirus)?
Turns gray; pupil becomes irregularly shaped
Use of heat lamp within an enclosure housing chickens (Gallus domesticus) may induce a fatal toxicosis due to severe adverse effects to which body system?
Respiratory (TEFLON aka polytetrafluoroethylene!)
See pulmonary hemorrhage
According to the Guide what is recommended dry bulb temperature for poultry?
16-27 C
61-81 F
(same for farm animals and poultry)
Production of polyclonal IgY antibodies in what species is an alternative to polyclonal IgG antibody production in rabbits?
Gallus domesticus (chicken)
According to Ag Guide what is recommended temp for hatchlings and piglets?
Piglets: 79-90F
Hatchlings: 90-95F
Chickens w/ sinusitis and conjunctivitis. Nx shows larynx/trachea inflamed, hemorrhagic, caseous cores. INIB in epithelium. Main ddx?
Infectious laryngotracheitis (gallid alphaherpesvirus 1 (GaHV-1))
= alphaherpesvirinae
Because they readily engage in reproductive behavior in the laboratory, which bird has served as a valuable model for the study of neuroendocrine control of reproductive and social behaviors?
Japanese quail
What type of antibodies are produced in people with hypersensitivity pneumonitis to bird serum or droppings?
IgG
It’s type III sensitivity! Antibody-antigen complexes
What pathogen can be transmitted through ova of Heterakis gallinarum (cecal roundworm) or through earthworms containing Heterakis larva?
Histomonas meleagridis (the protozoan)
What infections cause cause cheesy cecal cores?
Pullorum disease (Salmonella enterica serovar Gallinarum biotype Pullorum)
Blackhead disease (Histomonas meleagridis)
Fowl typhoid (Salmonella enterica serovar Gallinarum biotype Gallinarum)
What causes necrotic enteritis in chickens?
intestinal overgrowth of Clostridium perfringens Type A and C in young broilers and sometimes laying hens
Nutritional deficiency in ___ or ___ causes limb deformities.
Deficiency of manganese or choline
What endotracheal tube is used in birds?
Cole tube
What infection causes Yellow or white caseous lesions in the mouth, throat, or crop?
Trichomonas
How to treat Cochlosoma (protozoa) in zebra finches?
Ronidazole
If treating C. bovis in NSG mice, what is antibiotic of choice?
Clavamox x 14 days
What is the male-to-female ratio for achieving optimal fertility in battery cage-housed Coturnix japonica?
1 male to 3 females
According to the AVMA Guidelines for Euthanasia of Animals (2020 edition), which of the following species shows little or no aversion to inert gases?
Turkeys