RECOMBINATION AND TRANSFORMATION Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Antibiotic

A

Medications used to kill bacteria or slow their growth.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Antibiotic resistance gene

A

Gene which confers antibiotic

resistance.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Bacterial transformation

A

The process by which bacteria take up foreign DNA from their environment. Scientists use this process to introduce recombinant plasmids into bacteria.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Blunt end

A

The result of a straight cut across the double-stranded DNA by an endonuclease resulting in no overhanging nucleotides.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Electroporation

A

A method that involves delivering an electric shock to bacterial membranes to increase their membrane permeability and increase the likelihood of bacterial transformation.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Endonuclease

A

An enzyme that breaks the phosphodiester bond between two nucleotides in a polynucleotide chain.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Gene of interest

A

A gene scientists want to be expressed in recombinant bacteria. This gene often encodes a protein we wish to produce in commercial quantities. Also known as the desired gene.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Heat shock

A

A method that involves rapidly increasing and decreasing the temperature to increase membrane permeability in order to enhance the likelihood of bacterial transformation.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Overhanging nucleotides

A

Unbonded nucleotides on the ends of the DNA strand resulting from a staggered cut.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Plasmid

A

A small, circular loop of DNA separate from the chromosome, typically found in bacteria.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Recombinant plasmid

A

A circular DNA vector that is ligated to incorporate a gene of interest.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Reporter gene

A

Gene with an easily identifiable phenotype that can be used to identify whether a plasmid has taken up the gene of interest.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly