pcr Flashcards

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1
Q

anneal

A

The joining of two molecules, for example two complementary DNA strands during the cooling phase of PCR.

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2
Q

Forward primer

A

A technique that separates DNA fragments based on their molecular size.

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3
Q

Polymerase chain reaction (PCR)

A

A laboratory technique used to produce many identical copies of DNA from a small initial sample.

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4
Q

Primer

A

A short, single strand of nucleic acids that acts as a starting point for polymerase enzymes to attach.

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5
Q

Reverse primer

A

A DNA primer that binds to the 3’ end of the coding strand and reads the DNA in the reverse direction to RNA polymerase.

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6
Q

Taq polymerase

A

A heat-resistant DNA polymerase enzyme isolated from the bacteria Thermus aquaticus, which amplifies a single-stranded DNA molecule by attaching complementary nucleotides in PCR (at 72°C).

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