Random Tests

Question 1

What is the Shultz-Dale reaction?

Answer 1

A method for demonstrating anaphylactic hypersensitivity outside the body by suspending an excised intestinal loop or uterine strip from a sensitized animal in an oxygenated, physiological salt solution; addition of the proper allergen causes contraction of the smooth muscle

Question 2

What is the preyer reflex?

Answer 2

The elicitation of startle response to auditory stimuli

Question 3

What is the Galton response?

Answer 3

Galton attempted to measure intelligence through reaction time tests

Question 4

What is the LAL (limus amebocyte lysate) test?

Answer 4

Known as bacterial endotoxin testing.
In vitro assay used to detect the presence and concentration of bacterial endotoxins in drugs and biological products, and is an important part of pharmaceutical microbiology.

Question 5

Cons of Pyrogen testing?

Answer 5

Not appropriate for steroids or other medicines
Endotoxins cannot be quantified
Products that contain blood plasma have inactive form of endotoxins and won’t cause a fever

Question 6

Cons of LAL testing?

Answer 6

Can only detect endotoxins and not other pyrogenic materials

Question 7

FETAX is used to screen for what compounds?

Answer 7

Teratogens.

Assess mortality, malformation, and growth inhibition of developing embryos. South African clawed frog (Xenopus laevis)

Question 8

What does FETAX stand for?

Answer 8

Frog Embryo Teratogenesis Assay-Xenopus

Question 9

Montenegro tests detects which disease?

Answer 9

Leishmaniasis

Similar to purified protein derivative PPD used for Mycobacterium Tuberculosis

Question 10

What does the Water Flea Test measure?

Answer 10

Toxicity test for surface of water.
Part 1: acute toxicity observing immobility and mortality
Part 2: chronic toxicity observing survival and reproduction

Question 11

What does the sperm motility assay measure

Answer 11

Measures quality control in IVF lab. Conducted at 2, 4, 6, 8, and 24hr incubation intervals. <0.75 index value indicates sperm toxicity.

Question 12

What do chick chorioallantoic membrane (CAM) angiogenesis assays measure?

Answer 12

Study angiogenesis (neovascularization), tumor cell invasion, and metastasis

Question 13

Which of these is not autosomal recessive?

1. Dachs chondrodystrophy
2. Hypoplasia pelvis
3. Osteopetrosis
4. Femoral luxation
5. Achondroplastic chondrodystorphy

Answer 13

Femoral luxation is autosomal dominant

Question 14

What tear test is used in small mammals due to a small palpebral fissure?

Answer 14

phenol red thread tear test

75mm long cotton thread impregnated with phenol dye. Test for 15 second and measure part of thread that has changed

Question 15

What test measures the aqueous fraction of the tear film?

Answer 15

Endodontic adsorbent paper point tear test. The wetting is measured after 1 minutes. Also suitable for small eyes or low tear production.

Question 16

Benefit of using TonoVet?

Answer 16

Topical anesthetic not required and faster results than Tono-Pen

Question 17

What test is used to determine virulence of Listeria organisms?

Answer 17

Anton test (hemolysin in blood agar)

Question 18

What test is used to determine the mutagenic potential of chemical compounds?

Answer 18

Ames

Question 19

What test is used to determine the invasiveness of E coli, Shigella, and Listeria when inoculated into the guinea pig eye?

Answer 19

Sereny

Question 20

Rabbit pyrogen test evaluates what condition?

Answer 20

Bacterial endotoxin

Question 21

One of the first alternative tests used to replace the use of rabbits in what test?

Answer 21

Eye irritancy test