NHP DNA Viruses

Question 1

What is the family and genus for Monkeypox

Answer 1

Family: Poxviridae, Genus: Orthopoxvirus

Question 2

Vaccination against this virus confers immunity to monkeypox in most cases

Answer 2

smallpox

Question 3

T or F, Monkeypox is zoonotic

Answer 3

True. A 2003 outbreak from Prairie dogs that acquired it from gambian pouched rat

Question 4

Lesions of Monkeypox include vesicular exanthema on the hands, feet and face and can also involve the___________

Answer 4

Respiratory Tract where it causes a hemorrhagic necrosis

Question 5

This orthopoxvirus was eradicated through intensive vaccination programs in the 1970s

Answer 5

Variola (smallpox)

Question 6

_____ is the live attenuated Orthopoxvirus used in the small pox vaccine

Answer 6

Vaccinia

Question 7

What is typical histologically of a pox virus infection

Answer 7

Eosinophilic intracytoplasmic inclusions

Question 8

What is the typical appearance of a pox virus on electron microscopy

Answer 8

“dumbbell” appearance to viral particles

Question 9

T or F, Cowpox is Zoonotic

Answer 9

True

Question 10

what species is resistant to the pox virus, Yaba Monkey Tumor Virus

Answer 10

New World Primates

Question 11

What is the main reservoir for Monkeypox

Answer 11

African squirrel species

Question 12

How is monkeypox spread

Answer 12

aerosol, direct contact and biting insects

Question 13

Monkey pox viremia occurs how many days post infection

Answer 13

3-4days

Question 14

The classic pox lesion of Monkeypox occurs ____ days post infection

Answer 14

6-7

Question 15

What type of facility do you need for colony management of monkeypox

Answer 15

ABSL3; BSL2 facility with BSL3 practices

Question 16

What species have a natural infection to Yaba Virus

Answer 16

Macaques and baboons

Question 17

Yaba monkey tumor virus and yaba-like disease virus belong to what genus

Answer 17

Yatapoxvirus

Question 18

What does the Yaba virus infect

Answer 18

histiocytes

Question 19

T or F, Yaba Virus is zoonotic

Answer 19

True

Question 20

What are the clinical signs of Yaba Virus

Answer 20

Multiple SQ masses called “pseudotomors” that resolve in 1-2 months. Baboons get oral masses

Question 21

what virus occurs as a common, latent and typically asymptomatic infection in Asian macaques, but causes ascending paralysis and has a high fatality rate in humans

Answer 21

Macacine herpesvirus 1, Herpes B Virus

Question 22

How is Herpes B virus transmitted

Answer 22

sexual or biting behavior and by fomites

Question 23

What are clinical signs of Herpes B Virus

Answer 23

Usually subclinical, lesions included conjunctivitis, vesicles/ulcers on oral and genital mucosa

Question 24

Where does Herpes B establish latency

Answer 24

Trigeminal and lumbosacral sensory ganglia

Question 25

You should assume that all macaques and their products are McHV-1 positive

Answer 25

True