Quizfinals

Question 1

It is a gel free that has an advantage due.
to its superior separation efficiency,
small sample consumption and short
analysis time.
a. 2D-GE
b. Capillary Electrophoresis
c. HPLC
d. SDS-PAGE

Answer 1

a. 2D-GE

Question 2

This reagent is added to increase the
sensitivity of of detection
a. Coomassie Blue
b. BCA
c. NaOH
d. Folin-Ciocalteu reagent

Answer 2

a. Coomassie Blue

Question 3

This colorimetric assay is read at 562nm.
a. BCA
b. Lowry
c. Biuret
d. Bradford

Answer 3

a. BCA

Question 4

If you want to examine the
three-dimensional structure of a protein,
you will look into which database?
a. Genbank
b. PDBe
c. Signal transduction databases
d. Ensembl

Answer 4

b. PDBe

Question 5

Which of the following is a nucleotide
sequence database?
a. TREMBLE
b. PDBj
c. SWISS PROT
d. EMBL

Answer 5

d. EMBL

Question 6

When you get a BLAST for a DNA
sequence, the graphic summary refers to
a. The experimental three-dimensional
structure of a protein
b. A diagram representing evolutionary
relationships among organisms
c. The red bars specifying sequences
found in the database aligns to your
sequence.
d. A genetic map that shows details of
gene location relative to another
gene in the chromosome

Answer 6

c. The red bars specifying sequences
found in the database aligns to your
sequence.

Question 7

In a sequence alignment, a dot between
matched bases indicates
a. The bases are conserved across all
members of a species
b. A gap in the sequence
c. Identical bases
d. Non-identical bases

Answer 7

b. Non-identical bases

Question 8

The alignment of a procedure that tries
to align regions with a high level of
matches without considering the
alignment of the rest of the sequences.
a. Multiple sequence alignment
b. Pairwise alignment
c. Global alignment
d. Local alignment

Answer 8

c. Global alignment

Question 9

You have sequenced five PCR products
of DNA samples of escherichia coli you
have isolated from clinical specimens.
You want to determine in which
nucleotide bases the five DNA
sequences vary, which tool will be most
helpful to you?
a. MUSCLE
b. BLAST
c. EMBOSS WATER
d. PrimerBlast

Answer 9

a. MUSCLE

Question 10

If you want to compare the amino acid
sequence of your protein with those that
are stored in the databases, which tool
will you use?
a. BLASTp
b. BLASTn
c. MUSCLE
d. EMBOSS

Answer 10

a. BLASTp

Question 11

It is a diagram representing evolutionary
relationships among organisms
a. Pedigree
b. Chromatogram
c. Phylogenetic tree
d. Algorithm

Answer 11

c. Phylogenetic tree

Question 12

This is a unique identifier given to an
DNA or protein sequence record to allow
tracking of different versions of that sequence and the associated sequence overtime in a single data repository
a. Score
b. Expect value
c. Accession number
d. Gl number

Answer 12

c. Accession number

Question 13

If you are studying a set of all RNA
molecules in a mouse, you are dealing
with
a. Metabolomics
b. Proteomics
c. Transcriptomics
d. GENOMICS

Answer 13

c. Transcriptomics

Question 14

Advantages/ s of using molecular
techniques for diagnosing infectious
diseases.
a. Rapid results are produced
compared with the classical
microbiological testing
b. Different strains may be identified
c. A and B
d. Neither A nor B

Answer 14

a. Rapid results are produced
compared with the classical
microbiological testing
b. Different strains may be identified

Question 15

Advantage/s of using whole genome
sequencing (WGS) for detecting MRSA
a. Virulence of the strain may be
assessed
b. Prediction of resistance is possible
c. A and B
d. Neither A nor B

Answer 15

a. Virulence of the strain may be
assessed
b. Prediction of resistance is possible

Question 16

Arthropod - Borne bacterial infection that
may be diagnosed thru PCR based
assays
a. Borrelia species
b. Chlamydia trachomatis
c. A and B
d. Neither A nor B

Answer 16

a. Borrelia species

Question 17

DNA target/ s for the molecular detection
of fungal infection
a. rDNA
b. Cytochrome B
c. A and B
d. Neither A nor B

Answer 17

a. rDNA
b. Cytochrome B

Question 18

Molecular technique/s used for the
diagnosis of fungal infection
a. NAATs
b. ISH
c. A and B
d. Neither A nor B

Answer 18

d. Neither A nor B

Question 19

Most widely applied NAAT for the
diagnosis of Mycoplasma pneumoniae is
a. Southern Blot
b. PCR
c. A and B
d. Neither A nor B

Answer 19

b. PCR

Question 20

Principle involved in MALDI- TOF MS
a. Chemiluminescence
b. Ionization
c. A and B
d. Neither A nor B

Answer 20

b. Ionization

Question 21

Toxin genes are the main targets of real -
time PCR for the diagnosis of infection
caused by;
a. Staphylococcus aureus
b. Streptococcus pneumoniae
c. A and B
d. Neither A nor B

Answer 21

b. Streptococcus pneumoniae

Question 22

Which of the following is extracted for
MALDI - TOF MS microbial identification?
a. Nucleic acids
b. Protein
c. A and B
d. Neither A nor B

Answer 22

b. Protein

Question 23

If one is handling a heparinized
peripheral blood for molecular assay, the
proper handling temperature for the said
sample is.
a. Frozen
b. On ice
c. Room temperature
d. Water bath temp

Answer 23

c. Room temperature

Question 24

If one is handling skin biopsy for
molecular assay, the proper handling
temperature for the said sample is
a. Room temperature
b. Frozen
c. On ice
d. Water bath temp

Answer 24

c. On ice

Question 25

If one handling a amniotic fluid for
molecular assay, the proper handling
temperature for the said sample is
a. Frozen
b. Water bath temp
c. On ice
d. Room temperature

Answer 25

d. Room temperature

Question 26

If one is handling tissues ( liver, lungs,
etc) for molecular assay, the proper
handling temperature for the said sample
is
a. Water bath temp
b. On ice
c. Room temperature
d. Frozen

Answer 26

b. On ice

Question 27

Main advantages of using interphase
a. Multiple probes are used
b. Incubation temperature is lowered
c. No cell culture is required

Answer 27

c. No cell culture is required