Quizfinals Flashcards
It is a gel free that has an advantage due.
to its superior separation efficiency,
small sample consumption and short
analysis time.
a. 2D-GE
b. Capillary Electrophoresis
c. HPLC
d. SDS-PAGE
a. 2D-GE
This reagent is added to increase the
sensitivity of of detection
a. Coomassie Blue
b. BCA
c. NaOH
d. Folin-Ciocalteu reagent
a. Coomassie Blue
This colorimetric assay is read at 562nm.
a. BCA
b. Lowry
c. Biuret
d. Bradford
a. BCA
If you want to examine the
three-dimensional structure of a protein,
you will look into which database?
a. Genbank
b. PDBe
c. Signal transduction databases
d. Ensembl
b. PDBe
Which of the following is a nucleotide
sequence database?
a. TREMBLE
b. PDBj
c. SWISS PROT
d. EMBL
d. EMBL
When you get a BLAST for a DNA
sequence, the graphic summary refers to
a. The experimental three-dimensional
structure of a protein
b. A diagram representing evolutionary
relationships among organisms
c. The red bars specifying sequences
found in the database aligns to your
sequence.
d. A genetic map that shows details of
gene location relative to another
gene in the chromosome
c. The red bars specifying sequences
found in the database aligns to your
sequence.
In a sequence alignment, a dot between
matched bases indicates
a. The bases are conserved across all
members of a species
b. A gap in the sequence
c. Identical bases
d. Non-identical bases
b. Non-identical bases
The alignment of a procedure that tries
to align regions with a high level of
matches without considering the
alignment of the rest of the sequences.
a. Multiple sequence alignment
b. Pairwise alignment
c. Global alignment
d. Local alignment
c. Global alignment
You have sequenced five PCR products
of DNA samples of escherichia coli you
have isolated from clinical specimens.
You want to determine in which
nucleotide bases the five DNA
sequences vary, which tool will be most
helpful to you?
a. MUSCLE
b. BLAST
c. EMBOSS WATER
d. PrimerBlast
a. MUSCLE
If you want to compare the amino acid
sequence of your protein with those that
are stored in the databases, which tool
will you use?
a. BLASTp
b. BLASTn
c. MUSCLE
d. EMBOSS
a. BLASTp
It is a diagram representing evolutionary
relationships among organisms
a. Pedigree
b. Chromatogram
c. Phylogenetic tree
d. Algorithm
c. Phylogenetic tree
This is a unique identifier given to an
DNA or protein sequence record to allow
tracking of different versions of that sequence and the associated sequence overtime in a single data repository
a. Score
b. Expect value
c. Accession number
d. Gl number
c. Accession number
If you are studying a set of all RNA
molecules in a mouse, you are dealing
with
a. Metabolomics
b. Proteomics
c. Transcriptomics
d. GENOMICS
c. Transcriptomics
Advantages/ s of using molecular
techniques for diagnosing infectious
diseases.
a. Rapid results are produced
compared with the classical
microbiological testing
b. Different strains may be identified
c. A and B
d. Neither A nor B
a. Rapid results are produced
compared with the classical
microbiological testing
b. Different strains may be identified
Advantage/s of using whole genome
sequencing (WGS) for detecting MRSA
a. Virulence of the strain may be
assessed
b. Prediction of resistance is possible
c. A and B
d. Neither A nor B
a. Virulence of the strain may be
assessed
b. Prediction of resistance is possible
Arthropod - Borne bacterial infection that
may be diagnosed thru PCR based
assays
a. Borrelia species
b. Chlamydia trachomatis
c. A and B
d. Neither A nor B
a. Borrelia species
DNA target/ s for the molecular detection
of fungal infection
a. rDNA
b. Cytochrome B
c. A and B
d. Neither A nor B
a. rDNA
b. Cytochrome B
Molecular technique/s used for the
diagnosis of fungal infection
a. NAATs
b. ISH
c. A and B
d. Neither A nor B
d. Neither A nor B
Most widely applied NAAT for the
diagnosis of Mycoplasma pneumoniae is
a. Southern Blot
b. PCR
c. A and B
d. Neither A nor B
b. PCR
Principle involved in MALDI- TOF MS
a. Chemiluminescence
b. Ionization
c. A and B
d. Neither A nor B
b. Ionization
Toxin genes are the main targets of real -
time PCR for the diagnosis of infection
caused by;
a. Staphylococcus aureus
b. Streptococcus pneumoniae
c. A and B
d. Neither A nor B
b. Streptococcus pneumoniae
Which of the following is extracted for
MALDI - TOF MS microbial identification?
a. Nucleic acids
b. Protein
c. A and B
d. Neither A nor B
b. Protein
If one is handling a heparinized
peripheral blood for molecular assay, the
proper handling temperature for the said
sample is.
a. Frozen
b. On ice
c. Room temperature
d. Water bath temp
c. Room temperature
If one is handling skin biopsy for
molecular assay, the proper handling
temperature for the said sample is
a. Room temperature
b. Frozen
c. On ice
d. Water bath temp
c. On ice
If one handling a amniotic fluid for
molecular assay, the proper handling
temperature for the said sample is
a. Frozen
b. Water bath temp
c. On ice
d. Room temperature
d. Room temperature
If one is handling tissues ( liver, lungs,
etc) for molecular assay, the proper
handling temperature for the said sample
is
a. Water bath temp
b. On ice
c. Room temperature
d. Frozen
b. On ice
Main advantages of using interphase
a. Multiple probes are used
b. Incubation temperature is lowered
c. No cell culture is required
c. No cell culture is required
