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molbio > MOLBIO PRELIMS > Flashcards

MOLBIO PRELIMS Flashcards

1
Q

What biosafety level is exclusive for
primary health services, diagnostic services,
research?

A

Basic Biosafety Level 2

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2
Q

What is the ideal depth of the pipet tip to the
solution?

A

-1 to 3mm

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3
Q

In what circumstances you will manually
input the pH in the pH meter?

A

If the temperature differs from the standard
room temperature

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4
Q

Which of the following separates the DNA
strands in RNA synthesis?

A

Sigma subunit of RNA polymerase

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5
Q

Determine the molarity for 98000 mg of
phosphoric acid, H3PO4, in 2.00 L of
solution (MW=97.994 g/mol)

A

0.5 M

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6
Q

Development of In – situ hybridization
technique is contributed to:

A

Mary – Lou Pardue and Joseph Gal

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7
Q

Which of the following biosafety cabinet
provides the worker the highest protection?

A

-Class III

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8
Q

Which of the following technique does not
need electrophoresis?

A

Dot blot

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9
Q

Which of the following solutions is NOT
used in cleaning the pH electrode probe?

A

Concentrated NaOH

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10
Q

Which of the following statement is
INCORRECT about Absorbance?

A

It is directly proportional to the transmitted
light logarithm.

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11
Q

Universal/ Standard Precautions require that

A

-Blood and body fluids from all patients be
considered infectious

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12
Q

A substance that inhibits the growth and
development of microorganisms without
necessarily killing them

A

Antiseptic

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13
Q

Which of the following definitions best
describes disinfection?

A

The use of antimicrobial agents on
inanimate objects to destroy all non-sporeforming organisms

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14
Q

It is used to purify the granulocytes in the
Ficoll-Directed Gradient Extraction

A

Ammonium chloride

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15
Q

The following are examples of Personal
Protective Equipment EXCEPT:

A

Prescription glasses

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16
Q

Absorbance is also known as?

A

Optical density

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17
Q

Blotting technique for macromolecules that
uses electric current is:

A

Western blotting

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18
Q

What is the volume capacity of P20 pipet?

A

2 to 20 ul

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19
Q

Once the liquid is removed from the DNA
how can it be stored for years?

A

Keep in freezer

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20
Q

What bond does RNases break to degrade
RNA?

A

Phosphodiester bond

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21
Q

The containment principles, technologies
and practices that are implemented to
prevent the intentional exposure to
pathogens and toxin, or their intentional
release

A

Biosecurity

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22
Q

Immediate information concerning the
health hazards, flammability, and reactivity
of a chemical can be obtained from the

A

NFPA symbol

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23
Q

Assurance that the sensitive and valuable
information stored in a laboratory is
protected from theft

A

-Information security

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24
Q

It is a chaotropic agent used in protein
degradation

A

Guanidinium thiocyanate

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25
Q

What is the role of protease in DNA
extraction?

A

-Used to break polypeptides to small
fragments

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26
Q

Which of the following is in the correct
order regarding DNA extraction?

A

Cell lysis; RNAase treatment; protease
treatment &; ethanol precipitation

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27
Q

pH value is temperature dependent.

A

TRUE

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28
Q

The HEPA filters in a BSC filter particulate
to size () and are () efficient.

A

0.3µm, 99.97%

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29
Q

To decontaminate Class I and Class II
cabinets, this method should be utilized

A

Fumigation with formaldehyde gas

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30
Q

Sample problem: If O.D. at 260 is 0.30 nm
O.D at 280 is 0.45 nm Dilution factor is 100.
Compute for dsDNA concentration.

A

1500 ug per ml

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31
Q

Example of RNase inhibitors

A

DEPC

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32
Q

Termination of transcription is facilitated by

A

Rho factor

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33
Q

It is used to digest the cell walls of fungal
cells

A

Zymolyase

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34
Q

Which of the following is NOT a step in
DNA replication?

A

Splicing

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35
Q

Adenine forms a covalent bond with a
nucleic acid sugar at

A

N9

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36
Q

This is considered as the backbone of
Biosafety

A

Risk assessment

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37
Q

Which substance was used to break down
the phospholipids from the cell membrane
and nuclear membrane?

A

SOAP

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38
Q

What substance can cause the DNA to
precipitate?

A

Ethanol

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39
Q

During electrophoresis, denaturation of the
double stranded DNA molecule is brought
by:

A

Treatment with alkali

40
Q

How many grams would you prepare in
100ml of a 7.5% (w/v) solution of NaCl?

A

7.5g

41
Q

If the ratio between OD 260 and OD 280 is
0.63 what does that tell us?

A

The sample is contaminated with other
proteins

42
Q

What is the purpose of adding Proteinase K
in FFPE?

A

To increase the DNA yield

43
Q

Which of the following RNase inhibitors
needs to be removed from final preparation?

A

DEPC

44
Q

This equipment is used to accurately
measure and dispense small volumes of
liquid

A

Pipettors

45
Q

What is the main difference between RNA
and DNA?

A

RNA has ribose DNA has deoxyribose

46
Q

When does splicing occur?

A

Following pre-mRNA transcription in the
nucleus

47
Q

Which of the following is the basis for
Western blot?

A

Antigen – Antibody reaction

48
Q

Which of the following practices should be
utilized when working in a biological safety
cabinet?

A

Allow the BSC to operate before work
begins and after work ceases

49
Q

It is the most effective and reliable means to
sterilize laboratory materials and
decontaminate waste materials

A

Steam under pressure

50
Q

What is the importance of magnetic beads in
RNA extraction?

A

RNA binding

51
Q

What is the next step after the addition of
Elution buffer?

A

Add TE buffer

52
Q

Why Bunsen burner must not be used in the
biosafety cabinet?

A

The heat produced will distort the airflow
and may damage filters

53
Q

Sample problem: If O.D. at 260 is 0.30 nm
O.D at 280 is 0.45 nm Dilution factor is 100.
Check for the contamination.

A

0.67 nm

54
Q

Which of the following biosafety cabinet
does not have HEPA filtered supply air?

A

Class I

55
Q

RNA molecules that carry amino acids to the
ribosome

A

tRNA

56
Q

Dilute 150mL of 10X TBE buffer stock to
1.5L with deionized water.

A

-1X

57
Q

What level of biosafety practice Good
Microbiological Techniques plus protective
clothing only?

A

BSL 1

58
Q

The following terminate protein synthesis
EXCEPT

A

UGG

59
Q

Which of the following is FALSE for
RNases?

A

Preserve RNA in the sample

60
Q

Southern blotting is a techniques used to:

A

Identify specific DNA

61
Q

Equipment used in Molecular Biology to
visualize DNAs and proteins

A

UV transilluminator

62
Q

What is the tool that we used to measure pH
in our laboratory activity?

A

-pH meter

63
Q

In reagent preparation activity, what
instrument is used to measure the weight of
the chemicals?

A

Analytical balance

64
Q

Which of the following statement/s is/are
true regarding Southern blotting?

A
  • Developed by Dr. Edwin Southern
    -DNA – DNA hybridization is the basis
    -Transfer of DNA fragments from gel to
    nitrocellulose membrane is done
65
Q

TRUE for handling and storage of
Biological waste

A

Must be placed in a bag marked with
biohazard symbol

66
Q

How many ml of a 2.50M NaOH solution
are required to make 525ml of a 1.50M
NaOH solution?

A

315ml

67
Q

Which of the following is NOT a function of
nucleic acid?

A

They distribute protein all throughout the
body

68
Q

Filters generally used in biological safety
cabinet to protect the laboratory worker
from particulates and aerosols generated by
microbiology manipulations are

A

HEPA

69
Q

Autoclave temperature

A

-121°C

70
Q

ALL pipets have volume adjustment knob.

A

FALSE

71
Q

Calculate the molarity of 500ml of 1.9 x
10^-6moles of NaCN in a

A

0.0000038 M

72
Q

What does DNA stand for?

A

Deoxyribonucleic acid

73
Q

A microorganism that is unlikely to cause
human or animal disease

A

Risk Group 1

74
Q

Labelled antibodies are used to detect:

A

Presence of a particular Protein molecule in
Western Blot

75
Q

Which of the following statements is
FALSE?

A

Pipetting of BSL-3 materials should be
done in an open bench top

76
Q

How many grams of sodium chloride are
needed to prepare 1L of 0.9% normal saline?

A

9

77
Q

DNAs are found in a prokaryotic cell.

A

TRUE

78
Q

It is a multifaceted cellular response in
which more than 40 genes are induces when
DNA is damaged

A

SOS repair

79
Q

The first step in this technique is to depress
the plunger down to 1st stop.

A

Forward pipetting

80
Q

Which of the following is neutral pH?

A

7.00

81
Q

Where does protein synthesis takes place?

A

Ribosome

82
Q

In the final step of purification, DNA is
rehydrated with what type of buffer?

A

Tris-EDTA

83
Q

A technologist spilled 10 gallons of
formaldehyde on the floor. After
determining the chemical poses a significant
health hazard, the first action step would be
to:

A

-Evacuate the area

84
Q

The first step in this technique is to depress
the plunger down to 2nd stop.

A

Reverse pipetting

85
Q

Which of the following appears as a film of
debris at the interphase after centrifugation
of Organic Extraction Method?

A

DNA

86
Q

Which of the following is not true for
TRIzol?

A

-used for purification step

87
Q

The principle of capillary transfer is applied
in which technique?

A

Northern Blot

88
Q

Which of the following is INCORRECT
when it comes to PH meter handling?

A

2 point calibration is better that 3 point
calibration

89
Q

After centrifugation, it is the solid form
found at the bottom of the container

A

Precipitate

90
Q

For a safe operation of a centrifuge

A

Ensure proper balance is maintained

91
Q

This type of extraction relies on the
adsorption of nucleic acids to the solid phase

A

Minicolumn purification

92
Q

In Western blotting:

A

Polyacrylamide gel is commonly used

93
Q

When the hand is visibly soiled, it is
recommended to decontaminate the hands
with

A

-Soap and water

94
Q

Which of the following steps is arranged
accordingly?

A

Mix solution to be measured; Remove
electrode from storage buffer solution; Wash
the electrode with distilled water

95
Q

A solution is 0.2500M H2SO4. Calculate the
volume in ml present in a sample of the
solution that contains 0.005216moles of
sulfuric acid

A

20.864ml

96
Q

What is the volume capacity of P1000 pipet?

A

-200 to 1000 ul

97
Q

Which of the following does not describe
Guanidine thiocyanate-phenol-chloroform
extraction?

A

DNA molecules and proteins remain in the
upper layer of the tube

molbio (17 decks)

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