Molbio midtermquiz Flashcards
After heating and cooling the mixture, which of the following catalyzes the synthesis of complementary strands of
DNA?
a. ribonucleotides
b. Taq polymerase
c. deoxyribonucleotides
d. DNA molecules
Taq polymerase
DNA is visualized by including in the gel an intercalating dye is:
a. eosin dye
b. basic dye
c. ethidium bromide.
d. methylene blue
ethidium bromide.
Pfu polymerase is superior over Taq polymerase because:
a. of lesser quantity of enzyme required
b. of lower temperature required
c. of faster polymerase activity
d. of more accurate amplification
of more accurate amplification
Polymerase used for PCR is extracted from _____________.
a. Homo sapiens
b. Escherichia coli
c. Thermus aquaticus
d. Saccharomyces cerevisiae
Thermus aquaticus
A method that requires a set of four specifically designed primers that can recognize six distinct regions on the
target DNA
a. SDA
b. LAMP
c. RPA
d. RCA
LAMP
A technique used to amplify multiple target sequences in a single PCR reaction using multiple primer sets.
a. Loop Mediated Isothermal Amplification
b. Real-Time PCR
c. Conventional PCR
d. Multiplex PCR
Multiplex PCR
An enzyme that uses RNA as the template to make complementary DNA (cDNA)
a. DNA Polymerase
b. Taq Polymerase
c. Oligo Dt primer
d. Reverse Transcriptase
Reverse Transcriptase
Fidelity in amplification may be decreased in which of the following conditions?
a. Increased concentrations of Taq polymerase
b. Increased concentrations of dNTPs
c. Decreased concentrations of dNTPs
d. Decreased MgCl2
Increased concentrations of dNTPs
It is adjusted to a value above the background of the reaction
a. Sample
b. Threshold
c. Internal control
d. Baseline
Threshold
Low to no yield of PCR products may be a result of which of the following
a. Decreased MgCl2 and Increased dNTPs
b. Increased MgCl2 and Decreased dNTPs
c. None
d. Decreased MgCl2 and Decreased dNTPs
Decreased MgCl2 and Decreased dNTPs
Small fluorescent molecules that are attached to oligonucleotides in order to function as probes
a. Reporter
b. Fluorescence
c. Fluorophores
d. Quencher
Fluorophores
Their mechanism of action relies on the 5′–3′ exonuclease activity of Taq polymerase
a. Hydrolysis Probe
b. Analogues of nucleic acids
c. Scorpion Primer probe
d. Cyclicons
Hydrolysis Probe
This PCR technique was designed to to improve sensitivity and specificity.
a. Loop Mediated Isothermal Amplification
b. Nested PCR
c. Multiplex PCR
d. Real-Time PCR
Nested PCR
What would be the effect on the PCR reaction if any of the following circumstances arose:
1) there are no primers in
the reaction,
2) enough dNTPs
3) there is Taq polymerase in the reaction?
a. Non-specific PCR of random templates will occur
b. PCR would proceed normally
c. Amplicons will not be produced, false negative result
d. The reaction will cease after a few cycles
Amplicons will not be produced, false negative result
Which consists of primer molecules that have attached to each other because of strings of complementary bases
in the primers?
a. Primer dimers
b. Hairpin loops
c. Thymidine dimers
d. Amplicons
Primer dimers
Which of the following PCR techniques is the gold standard for testing COVID-19 infection?
a. Real-Time PCR
b. Loop Mediated Isothermal Amplification
c. Multiplex PCR
d. Conventional PCR
Real-Time PCR
What is the difference between a deoxyribonucleotide and a dideoxyribonucleotide?
a. A deoxynucleotide is missing a 5’-phosphate group.
b. A deoxynucleotide is missing a 3’-hydroxyl group on its sugar.
c. A dideoxynucleotide is missing a 5’-phosphate group.
d. A dideoxynucleotide is missing a 3’- hydroxyl group on its sugar.
d. A dideoxynucleotide is missing a 3’- hydroxyl group on its sugar
To sequence DNA by Maxam-Gilbert method, the DNA must first be made in single stranded form.
a. TRUE
b. FALSE
TRUE
Which of the following is a chemical sequencing method?
a. DNA chip sequencing
b. Real-time PCR
c. Sanger
d. Maxam-Gilbert
Maxam-Gilbert
How many types of dDNTPs are used in Sanger technique?
a. 3
b. 4
c. 2
d. 1
4
When setting up a Sanger sequencing reaction, each reaction should include template DNA, deoxyribonucleotides,
dideoxyribonucleotides, buffer, DNA polymerase, and _______.
a. Forward AND reverse DNA probes
b. Forward OR reverse DNA probes
c. Forward AND reverse primers
d. Forward OR reverse primer
Forward OR reverse primer
When a dideoxyribonucleotide is added to the DNA strands being synthesized:
a. replication of the strand is speeded up
b. replication of the strand stops
c. replication of the strand is not affected
d. replication of the strand continues
replication of the strand stops
What is the most widely used sequencing technique principle?
a. Chemical modification sequencing
b. A and B
c. Maxam-Gilbert sequencing
d. Chain termination sequencing
Chain termination sequencing
To sequence DNA by Sanger method, the DNA must first be made in single stranded form.
a. TRUE
b. FALSE
TRUE
Which of the following sequencing techniques requires strands amplification?
a. Chemical modification sequencing
b. Chain termination sequencing
c. Maxam-Gilbert sequencing
d. A and
Chain termination sequencing
Mrr
a. Type IV
b. Type III
c. Type I
d. Type II
Type IV
ATP, SAM, Mg2+
a. Type I
b. Type IV
c. Type III
d. Type II
Type I
Cleaves at sites away from recognition site
a. Type III
b. Type II
c. Type IV
d. Type I
Type I
Cleave at sites 25-27 bp from recognition site
a. Type III
b. Type IV
c. Type I
d. Type II
Type III
EcoB
a. Type II
b. Type IV
c. Type III
d. Type I
Type I
Cleave close to or within recognition sequence
a. Type III
b. Type IV
c. Type II
d. Type I
Type IV
Cleave within or at a short distance from the recognition site
a. Type IV
b. Type I
c. Type II
d. Type III
Type II
ATP, Mg2+
a. Type III
b. Type II
c. Type IV
d. Type I
Type III
EcoPI
a. Type I
b. Type II
c. Type IV
d. Type III
Type III
BamHI
a. Type IV
b. Type I
c. Type III
d. Type II
Type II
