Quiz Review Flashcards
ecn of alp:
3.1.3.1
-activators of alp:
mg&zn
-fastest factor isoenzyme
Liver
-most heat stable:
placental alp
Or REGAN??
-neoplastic isoenzyme for
lung, breast, ovarian, colon
cancers:
regan isoenzyme
Bone Disorders:
Highest elevations in
Paget’s disease (Osteitis deformans)
-optimal pH sa ACP:
5.0
-best method to detect isoenzymes:
most useful technique for isoenzyme analysis
electrophoresis
-: Richest source, highest activity ACP:
Prostate
-nkalimot ko sa question basta ang answer kay:
Historically used for detecting metastatic carcinoma of the prostate Less sensitive than newer markers like Prostate-Specific Antigen (PSA)
Prostatic Carcinoma Detection
Chemical inhibition
Uses substrates like thymolphthalein monophosphate
Differentiates_______ using tartrate inhibition Equation: Total ACP - ACP after tartrate inhibition =_____
prostatic ACP
Chemical Inhibition:
Uses substrates like______
Differentiates prostatic ACP using_____
thymolphthalein monophosphate
tartrate inhibition
Formula
= Prostatic ACP
Total ACP - ACP after tartrate inhibition
Not entirely specific, as lysosomal ACP is also inhibited by tartrate
Tartrate inhibition
Roy and Hillman (Quantitative Endpoint)
Substrate:
Thymolphthalein monophosphate