Phosphatase Agappe

Question 1

ALP

Reagent Preparation
Mix R1 with R2

The working reagent is stable for 30 days at____

Answer 1

4 volume of Reagent 1 (R1) with 1 volume of Reagent 2 (R2)

2-8°C.

Question 2

ALP sample

Answer 2

Sample

Serum/Plasma (free of haemolysis).

Question 3

ALP

Wavelength

Answer 3

405nm

Question 4

ALP Factor

Answer 4

2757

Question 5

ALP Linearity

Answer 5

2000 U/L

Question 6

ALP

sample volume
Reagent volume

Answer 6

20 uL
1000 uL

Question 7

ALP

Women

Answer 7

35 - 104 U/L

Question 8

ALP

Men

Answer 8

40 - 129 U/L

Question 9

ALP

Children up to15 yrs:

Answer 9

54 - 369 U/L

Question 10

ACP

________is hydrolysed by acid phosphatase (Ac. P.) to______ and ______, which is converted with FRTR-salt to an azo dye.

The increase of absorbance at______ is proportional to the total acid phosphatase activity in the sample.

Answer 10

1-Naphthyl phosphate

phosphate and 1-naphthol

405 nm

Question 11

ACP

Specimen

Answer 11

Serum, no plasma!
Avoid hemolytic and icteric sera!

Question 12

ACP

Temperature:

Answer 12

25°C, 30°C or 37°C

Question 13

Calculate the total acid phosphatase activity in the sample using the following factors:

25°C
30°C / 37°C

Answer 13

149
248

Question 14

ACP

Conversion: 1 U/I =

Answer 14

16.67 x 10^-3 ukat/I

Question 15

ACP

MEN up to

Answer 15

3.6 U/L
5.0 U/L
6.5 U/L

Question 16

ACP

WOMEN

Answer 16

3.0 U/L
4.2 U/L
5.5 U/L