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CC 2 LAB > Magnesium > Flashcards

Magnesium Flashcards

1
Q

MAGNESIUM
_____most abundant cation

A

Fourth

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2
Q

MAGNESIUM

____most abundant intracellular ion

A

Second

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3
Q

Most abundant cation

A
  1. Sodium
  2. Potassium
  3. Calcium
  4. Magnesium
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4
Q

Most abundant intracellular ion

A
  1. Potassium
  2. Magnesium
  3. Phosphate
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5
Q

FUNCTIONS

A

• Essential cofactor (>300 enzymes)
• Transcellular ion transport
• Neuromuscular transmission
• Synthesis of carbohydrates, proteins, lipids, and nucleic acid
• Release of and response to certain hormones

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6
Q

SPECIMEN CONSIDERATIONS

A

Serum (Non-hemolyzed)
Lithium Heparin Plasma
24-hour urine

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7
Q

Anticoagulants that will bind
with magnesium

A

Oxalate
EDTA
citrate

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8
Q

Why 24-hour urine?

A

Eliminates the interference caused by diurnal variation

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9
Q

24 hr urine

Must be acidified with___ to avoid___

A

HCl

precipitation

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10
Q

METHODS

A

• Colorimetric Methods
• Atomic Absorption Spectrophotometry

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11
Q

Colorimetric Methods

A

• Calmagite Method
• Formazen Dye Method
• Methylthymol Blue Method

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12
Q

Colorimetric Methods

• Mg+ binds with calmagite to form a reddish-violet complex that may be read at 532 nm

A

Calmagite Method

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13
Q

Colorimetric Methods
• Calmagite Method
• Mg+ binds with_____ to form a_____(color) that may be read at____

A

calmagite

reddish-violet complex

532 nm

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14
Q

Colorimetric Methods

• Mg+ binds with the dye to form colored complex that may be read at 660 nm

A

Formazan Dye Method

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15
Q

Colorimetric Methods
• Formazan Dye Method
• Mg+ binds with the___ to form____(color) that may be read at____

A

Formazan dye

colored complex

660 nm

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16
Q

Colorimetric Methods

• Mg binds with the chromogen to form a colored complex

A

Methylthymol Blue

17
Q

Colorimetric Methods
• Methylthymol Blue
• Mg binds with the______ to form a _____ (color)

A

chromogen - Methylthymol Blue

colored complex - Blue

18
Q

• Reference Method

A

Atomic Absorption Spectrophotometry

19
Q

Approximately 53% of Mg2+ in the body is found in____, 46% in_____, and less than 1% is present in_____.

A

bone

muscle and other organs and soft tissue

serum and RBCs

20
Q

Mg2+ concentration inside erythrocytes is____ times greater than that in the ECF, ____ should be avoided and the serum should be separated from the cells as soon as possible.

A

10

Hemolysis

21
Q

Magnesium must be analyzed…

A

As soon as possible

CC 2 LAB (30 decks)

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