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CC 2 LAB > Prelims Agappe > Flashcards

Prelims Agappe Flashcards

1
Q

AMYLASE (AMS)

A

EC 3.2.1.1

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2
Q

LIPASE (LPS)

A

EC 3.1.1.3

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3
Q

CREATINE KINASE (CK)

A

EC 2.7.3.2

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4
Q

LACTATE DEHYDROGENASE (LDH)

A

EC 1.1.1.27

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5
Q

ASPARTATE AMINOTRANSFERASE (AST)

A

E.C. 2.6.1.1

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6
Q

ALANINE AMINOTRANSFERASE (ALT)

A

E.C. 2.6.1.2

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7
Q

ALKALINE PHOSPHATASE (ALP)

A

E.C. 3.1.3.1

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8
Q

ACID PHOSPHATASE (ACP)

A

E.C. 3.1.3.2

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9
Q

Activators of AMY

A

Calcium
Chloride

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10
Q

Lipase activators

A

Colipase
Bile salts

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11
Q

Aspartate and Alanine activators

A

Pyridoxal phosphate

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12
Q

Alkaline activators

A

Optimal pH: 9.0-10.0 Activator: Mg 2 + & Zinc

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13
Q

Acid activators

A

Optimal pH: 5.0 Activator = Mg 2 + & Zinc

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14
Q
  1. Amyloclastic method
  2. Saccharogenic method
  3. Chromogenic method
  4. Continuous monitoring method
A

Amylase

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15
Q
  1. Classic Cherry-Crandall Method (olive oil)
  2. Turbidimetric Methods
  3. Colorimetric Methods
A

Lipase

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16
Q

Electrophoresis
Immunoinhibition
Immunoassay
Ion-exchange chromatography

A

CK isoenzyme

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17
Q

Tanzer Gilvarg
Oliver Rosalki

A

CK enzyme

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18
Q

Wacker
Wrobleuski

A

Ldh

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19
Q

Karmen Method - Uses a coupled enzymatic reaction

Indicator: Malate Dehydrogenase

Monitors absorbance change at_____

  • Measures NADH oxidation to NAD+
  • Optimal pH Range:_____
A

Aspartate

340 nm

7.3 to 7.8

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20
Q

Coupled Enzymatic Reaction:

  • Utilizes Lactate Dehydrogenase (LD) as the indicator enzyme
  • LD catalyzes reduction of pyruvate to lactate
  • Simultaneous oxidation of NADH to NAD+
A

Alanine Aminotransferase

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21
Q

Reitman & Frankel

  • Colorimetric/Endpoint
  • Color developer:______
  • Color intensifier:_____
A

2,4 Dinitrophenylhydrazine (DNPH)

0.4N Sodium hydroxide (NaOH)

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22
Q

Reitman & Frankel

  • Colorimetric/Endpoint
A

Alanine Aminotransferase

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23
Q

Continuous-Monitoring Technique:

Based on Bowers and McComb method

24
Q

ALKALINE

Continuous-Monitoring Technique: Based on Bowers and McComb method

Most specific method Calculates ALP activity using molar absorptivity of p-nitrophenol

Substrate =_______

Positive result =______

A

para-Nitrophenylphosphate (PNPP); colorless

Yellow (p-nitrophenol)

25
Q

Roy and Hillman (Quantitative Endpoint) Substrate:

A

Thymolphthalein monophosphate

ACID

26
Q

Babson, Read & Phillips (Continuous Monitoring) Substrate:

A

Alpha-naphthyl phosphate

ACID

27
Q

Immunochemical Techniques:

  • Radioimmunoassay (RIA)
    -Counterimmunoelectrophoresis
  • Immunoprecipitation
  • Immunoenzymatic Assay (Tandem E)
28
Q

Amylase RS

29
Q

CK RS

30
Q

LDH RS

31
Q

AST
ALT

RS

32
Q

ALP

RS

33
Q

ACP

34
Q

CK

NR

A

SERUM: 25 - 86 U/L
URINE: < 470 U/L

35
Q

LIPASE NR

36
Q

CK NR

A

Serum up to : 24 U/L %
CK- MB :6-25 %

37
Q

LDH NR

1- 3 years :

A

490-730 U/L

38
Q

LDH NR

4 - 9 years :

A

320-520 U/L

39
Q

LDH NR

10 - 13 years :

A

250-500 U/L

40
Q

LDH NR

Adults :

A

225-450 U/L

41
Q

LDH NR

A

1- 3 years :490-730 U/L
4 - 9 years :320-520 U/L
10 - 13 years :250-500 U/L
Adults :225-450 U/L

42
Q

Aspartate NR

A

Serum up to 46 UL

43
Q

Alanine NR

A

Serum up to 49 U/L

44
Q

Alkaline NR

Women

A

35 - 104 U/L

45
Q

Alkaline NR

Men

A

40 - 129 U/L

46
Q

Alkaline NR

Children up to15 yrs:

A

54 - 369 U/L

47
Q

Alkaline NR

A

Women 35 - 104 U/L
Men 40 - 129 U/L
Children up to15 yrs: 54 - 369 U/L

48
Q

ACP NR Men

A

Men up to (U/I) 3.6; 5.0; 6.5

49
Q

ACP NR women

A

Women up to (U/I) 3.0; 4.2; 5.5

50
Q

Amylase factor

51
Q

CK factor

52
Q

LDH factor

53
Q

AST factor

54
Q

Alanine factor

55
Q

Alkaline factor

56
Q

Acid factor

A

25°C = 149 30°C or 37°C = 248

CC 2 LAB (35 decks)

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