Quiz 3 Flashcards
Pauling
chemical bonds, alpha helix (protein 2nd structure)
Chargaff
discovered the nucleotide content of DNA (%G=%C, %T=%A)
Wilkins
British biophysicist, worked on DNA structure
Franklin
X-ray diffraction studies of DNA
Watson and Crick
studied proteins at first, used pictures from Franklin, discovered the structure, double helix of DNA, made DNA models
DNA structure
backbone of nucleotides- thymine, adenine, guanine, cytosine, connected by covalent bonds
- two strands connected by hydrogen bonds
- double helix
RNA structure
- contains ribose sugar
- directly involved in making proteins
- contains uracil instead of thymine
- single strand
Sequence hypothesis
DNARNA->Proteins
Replication, transcription, translation
chromatin
structure of a chromosome consisting DNA wrapped around histone proteins
nucleosome
histone protein with their encircling DNA
replication
- process by which a cell makes copies of its DNA
- 2 strands of DNA unwind at many sites along the length of the molecule
- hydrogen bonds are broken by the enzyme helicase
- each parent strand serves as a template fro the assembly of a new DNA strand from nucleotides
- 2 stands (half old half new) of DNA are now present
codon
set of 3 nucleotides of mRNA that codes for the placement of an amino acid during translation
start codon
tells ribosome where the beginning of the gene is
stop codon
tells the ribosome when to stop
promoter sequence
specific sequences of DNA nucleotides that RNA polymerase uses to find a protein-coding region of DNA and to identify which of the two DNA strands is the coding strand
terminator sequence
DNA sequences that indicate when RNA polymerase should finish making an RNA molecule
anticodon
trio of bases in the tRNA that is involved in base-pairing
gene
- section of DNA that codes fro a protein or pieces of RNA and determines traits
- able to replicate by directing the manufacture of copies of themselves
- can get mutated
- stores information that determines the characteristics of cells and organisms
- uses information to direct synthesis of proteins
intron
sequences of mRNA that do not code for a protein
exon
sequences of mRNA that codes for a protein
mutation
any change in the DNA sequences of an organism
transposon
a non protein coding DNA
DNA polymerase
enzyme that connects nucleotides together
RNA polymerase
enzyme that “reads” the sequence of DNA that indicate the base pairing rules to build the new RNA molecule
transcription
- process of using DNA as a template to synthesize RNA
- RNA polymerase “reads” the DNA and attaches to it and begins to build a new protein
- beings when the enzyme separates the two strands of DNA
- exposes the nitrogen bases so one strand can be “read”
translation (protein synthesis)
process of using the information in RNA to direct protein synthesis by attaching amino acids to one another
imitation
start codon, AUG, end of tRNA carries an amino acid
elongation
chain of amino acids is growing, every time the ribosome works through a series, a new amino acid is added
termination
chain of amino acids grow until stopped by a stop codon, protein detaches from the ribosome
mRNA
messenger RNAm carries the blueprint for making the necessary protein
rRNA
ribosomal RNA, involved in the assembly of proteins from amino acids
tRNA
transfer RNA, moves a specific amino acid into a ribosome to make a protein
chromosomal aberration
major change in DNA that can be observed at the level of the chromosome that can cause harm, especially during fetal development
deletion
broken piece of DNA becomes lost or destroyed before its reattached
inversion
occurs hen a chromosome is broken and a piece becomes reattached to its original chromosome, but flipped
translocation
one broken segment of DNA becomes integrated into a different chromosome
duplication
portion of a chromosome is replicated and attached to the original
point mutation
change in a single nucleotide of the DNA sequence
silent mutation
nucleotide change that results in either the placement of the same amino acid or a different amino acid, but does not change the function of a protein
missense mutation
causes the wrong amino acid to be used in making a protein
nonsense mutation
causes a ribosome to stop protein synthesis by introducing a stop codon early
frameshift
occurs when insertion or deletions cause the ribosome to read the wrong sets of 3 nucleotides
Base pairing rules
DNA- A=T, G=C
RNA- A=U, G=C
genetic enginerering
manipulating DNA for our benefit
biotechnology
using organisms for our benefit