Quiz 1 Flashcards
10X Feathered edge
Microfilaria
WBC distribution
Abnormal cells
Platelet clumping
40X Feathered edge
nothing
100X Feathered edge
nothing
10X Monolayer
Appropriate size
WBC distribution
40X Monolayer
WBC differential
begin morphology
100X Monolayer
RBC morphology
WBC morphology
Platelet morphology
Platelet estimate
Lymphocyte
round to oval nucleus high nucleus to cytoplasm ratio agranulocyte most abundant in cow 55-85% of the diff in cows
Neutrophil
Segmented nucleus with dense chromatin Clear to dusty purple grey granules Granulocyte Most abundant in dogs/cats 55-85% of the diff in dogs/cats
Monocyte
Ameboid nucleus with “lacey” chromatin
equal or low nucleus to cytoplasm ratio
Agranulocyte
0-5% in all cells
Basophil
Segmented nucleus with dense chromatin
Blue/ blue grey granules
Granulocyte
Least common cell in all species
Eosinophil
Segmented nucleus with dense chromatin
Pink granules
Granulocyte
0-5% in all species
Blood film too thin
increase the angle
Blood film too thick
Decrease the angle
Diff Quik
Fixative 5, 1 second dips
Eosinophillic 5, 1 second dips
Basophilic 7-10, 1 second dips
Wright’s Stain
Wrights Stain 1 min.
Buffer + 1 pipette wrights 1 min.
Distilled H20 7-10, 1 second dips
Red
Hemolytic - Shaking
Yellow
Icteric - Carotenes
White
Lipemic - Hypothyroidism
Diff. between serum & plasma
Plasma is the fluid portion of anti-coagulated blood and Serum is the fluid portion of coagulated blood
4 ways to asses RBC morphology
Size
Shape
Color
+/- inclusions
Avian venipuncture site
Jugular <25ga
Cat venipuncture site
Jugular 22ga
Large dog venipuncture site
Jugular 20ga
Cow venipuncture site
Tail vein 20ga
Hog venipuncture site
AVC 16ga (3in)
PCV direct from pt.
RRCT
Contains heparin
CBC
LTT
Contains EDTA
Biochemistries
RTT
Contains nothing
Coagulation studies
BTT
Citrate
PCV in conj. with LTT
BRCT
Contains nothing
Blood gases
Green TT
Contains heparin
Blood glucose
Grey TT
Contains oxylate
PCV
a % quantity of blood that is made up of RBCs
Conventional time for PCV
5min. at 6000RPM
White layer between the plasma and the red cells in a HCT is the ____ & is made up of ____
buffy coat, WBC’s and Platelets
What is used to measure plasma proteins?
Accurate to the nearest ___g/dl
Refractometer
0.2g/dl
What fluid is used to clean the refractometer?
What is the specific gravity of this fluid?
Distilled H2O
1.000
Dog RBC normals
5-10
Cat RBC normals
5-11
Dog PCV normals
37-55%
Cat PCV normals
30-45%
Dog TP normals
5.0-7.0 g/dl
Cat TP normals
5.0-5.8 g/dl