FINAL EXAM Flashcards
What instrument is used to measure plasma proteins?
Refractometer
Refractometers are accurate to the nearest ___
0.2g/dl
Blue Ring capillary tube
Nothing
PCV/TP from LTT
Red Top Tube
Nothing
Biochems
Green Top Tube
Heparin
Blood gases
Blue Top Tube
Na Citrate
Coag studies
Gray Top Tube
Oxalate
Blood glucose
Red ring capillary tube
Heparin
PCV/TP direct from pt
Purple Top Tube
EDTA
CBC
Most common cause of error in hematology is
not mixing
T/F: A retic count is indicated to determine the bone marrows response to anemia in the horse
False
PCV & TP in a Hemorrhage pt
decreased PCV, decreased TP
PCV &TP in Dehydrated pt.
increased PCV, increased TP
PCV & TP in Hemolysis
decreased PCV, increased TP
PCV & TP in liver failure
normal PCV, decreased TP
PCV & TP in renal failure
decreased PCV, increased, decreased, or normal TP
3 mechanisms for absolute hypoproteinemia & example
Decreased production- Liver failure
Increased loss- Hemorrhage
Decreased consumption- Starvation
3 morphologic differences between avian and mammalian blood?
Heteros-Segs
Thrombocytes-Platelets
Polychrom. Common- Poly Uncommon
What are the 3 functions of platelets
Adhere
Aggregate
Secrete
Why do we stage tumors?
prognosis & treatment
What are the 4 sites for bone marrow sampling?
Wing of ilium
Proximal humerus
Proximal femur
What are the 3 mechanisms of anemia & examples
Increased loss- Hemorrhage
Decreased production- BM toxin
Increased destruction- IMHA
What is the most common cause of relative polycythemia?
Dehydration
Stress leukogram cause & WBC distribution
Glucocorticoids Neutros ^ Bands 0 Lymphs v Monos ^ Eos v Basos 0 N
Physiologic leukogram cause & WBC distribution
Epinephrine Neutros ^ Bands 0 Lymphs ^ Monos ^ Eos ^ Basos ^
Inflammation leukogram cause & WBC distribution
Bacterial products Neutros ^ Bands ^ Lymphs N Mono ^ Eos N Basos 0 N
List the abnormal colors of plasma, terms, and causes for each
Red-Hemolyic-Shaking
Yellow-Icteric-Biliary stasis
White-Lipemic-Post Prandial
T/F: Petechiae, ecchymosis, and mucosal bleeding are clinical signs of primary hemostatic disorders
True
The main cause of bleeding disorders in dogs is
Thrombocytopenia
List the 4 criteria for toxic change in neutrophils
Vacuoles in cytoplasm
Dohle bodies
Azurophilic granules
Diffuse basophilic cytoplasm
Neutrophil
Granulocyte
Allergy
May be toxic
Basophil
Granulocyte
Anaphylaxis
Eosinophil
Granulocyte
Allergy
Antiparasite
Monocyte
Recycles RBCs and WBCs
Agranulocyte
Lymphocyte
Agranlocyte
May be reactive
Antibody production
Platelet
Neovascularization
May be reactive
Primary hemostasis
Erythrocyte
O2 transport
Nucleated in birds
what are the 2 clotting factors that are the primary components of the extrinsic pathway
Tissue thromboplastin & VII
T/F: Hematomas and blood in body cavities are common signs of secondary hemostatic disorders
Trues
List 3 factors that are secreted from or are produced by platelets and give a function for each
TXA - vasoconstriction
PF3- stimulates coag. cascade
vWF & VIII- promotes aggregation
an ______ venipuncture is necessary to avoid introduction of tissue ____ in your sample when collecting samples for coag studies
Atraumatic
thromboplastin
List 3 mechanisms for thrombocytopenia and one example of each
Increase loss - Hemorrhage
Decreased production - BM toxin
Increased destruction - IM thrombocytopenia
Red Top Tube contents and pathways
Biochemistries
Intrinsic, Extrinsic, Common
Blue Top Tube contents and pathways
Primary hemostasis Citrate Intrinsic, Extrinsic, Common vonWillebrand's factor must be full
Diatomaceous Earth Tube
Must be full
ACT
Purple Top Tube contents, use and hemostasis type
EDTA
CBC
Platelet counts
Primary Hemostasis
Tissue thromboplastin activates the ____ limb of the coagulation cascade
Extrinsic
VonWillebrand’s Dz is a ____ disease
Inherited
Primary Hemostasis Disease and test used to diagnose
Thrombopathia - BMBT
Secondary Hemostasis Disease and test used to diagnose
vWD - PT
Fibrinolysis disease and test used to diagnose
DIC - FDP
Major crossmatch
2 drops donor RBC suspension
2 drops receipt plasma
Minor crossmatch
2 drops donor plasma
2 drops receipt RBC suspension
Control crossmatch
2 drops donor plasma & RBC suspension
End point observations of crossmatching
Hemolysis
Agglutination
Domestic cat blood type
A
Exotic cat blood type
B
Why exotic cats must be crossmatched
because they have a natural occurring anti A antibody
How much blood can a dog donate and how often?
10ml/lb
3 weeks
The preferred canine donor blood type is ___ which means they are lacking ___ antigens
A-
1.1, 1.2, 7
2 major categories of transfusion reactions and one cause of each
Non-Immunologic - Sepsis
Immunologic- delayed hypersensitivity
anticoagulant or anticoag & preservative?
Acid Citrate Dextrose
AP
anticoagulant or anticoag & preservative?
Heparin
A
anticoagulant or anticoag & preservative?
3.8% sodium citrate
A
Citrate phosphate dextrose with adenine
AP
What blood collection chemical has the longest shelf life?
How long is the shelf life?
Citrate Phosphate Dextrose with Adenine
35 days
What is the name of the product which delivers hemoglobin to the patient without the use of cells?
What are 2 advantages?
Oxyglobin
2 year shelf life
no transfusion reactions
What cell can dohle bodies happen in?
Neutrophils
What cells can have basophilic staining or cytoplasm
Neutro
Lymphs
Erythrocytes
Platelets
What cells can have heinz bodies?
Erythrocytes
What cells can have vacuolization
Neutros
Lymphs
Platelets
What cells can have Howell-Jolly Bodies
Erythrocytes
What cells can have Leptocytes
Erythrocytes
What cells can have Target cells
Erythrocytes
What cells can be reactive?
Platelets
Lymphs
What cells can be toxic?
Neutrophils
Preferred blood component for hemorrhagic shock pt
Whole blood
Preferred blood component for normovolemic anemic
Packed RBC
Preferred blood component for anemic with heart failure
Packed RBC
Preferred blood component for hypoproteinemic
Plasma
Preferred blood component for Warfarin intoxication, nonanemic
Plasma
Preferred blood component for vonWillebrand’s Dz
Cryopercipitate
What should be done to the recipient prior to a blood transfusion?
Crossmatch
CRT
TRP
What blood component has a 72 hour shelf life and isnt refrigerated
Platelet rich plasma
List in order the maturation series of the cell that forms platelets
Pleurapotential stem cell
Megakaryoblast
Promegakaryocyte
Megakaryocyte
What are the 3 components of hemostasis
Primary
Secondary
Fibrinolysis
What are the factors of the intrinsic pathway?
12, 11, 9, 8
What are the 2 factors of the extrinsic pathway?
tissue thromboplastin & 7
What are the vit. K dependent facotrs?
2, 7, 9, 10
What occurs in the common pathway?
prothrombin to thrombin, fibrinogen to fibrin
What are the 2 parts of primary hemostasis?
Vascular & platelet plug
What starts fibrinolysis?
Plasminogen
What is the by-product of fibrinolysis?
FDPs
List the order of maturation for the lymphocyte
Resting memory cell
Lymphoblast
Prolymphocyte
Lymphocyte
List the order of maturation for the monocyte
PPSC
Monoblast
Promonocyte
Monocyte
List the order of maturation for the neutrophil
PPSC Myeloblast Promyelocyte Myelocyte Metamyelocyte Band Mature Seg
List the oder of maturation for the erythrocyte
PPSC Rubriblast Prorubricyte Rubricyte Metarubricyte Polychromatophil Mature RBC
What observation must be made on the FE of the blood film to accurately interpret a platelet count? Why?
Clumping
What needle is used for a bone marrow aspirate?
parts of this needle
anticoagulant used
Rosenthal
Needle & stylet
EDTA
Needle used for BM core biopsy & parts
Jam Shidi
Cap, Needle, Stylet, Stylet hook
Normal MCV ranges
dog- 60-70fl
cat- 39-55fl
Normal PCV ranges
dog- 37-55%
cat- 30-45%
Normal MCHC ranges
30-36g/dl
Normal platelet ranges
200,000-500,000
Normal TP ranges
dog- 5.0-7.0 g/dl
cat- 5.0-8.0 g/dl