Questions from Postlab slides Flashcards
Infolding of cell sheet into embryo
Invagination
Inturning of cell sheet over the basal surface of an outer layer
Involution
Migration of individual cells into the embryo
Ingression
Splitting of one sheet into two sheets
Delamination
Expansion of one cell sheet over other cells
Epiboly
Contact and recognition between sperm and egg
Fertilization
Mitosis without size increase
Cleavage
Ending of cleavage
Formation of blastoderm
Complete cleavage
Holoblastic
Incomplete cleavage
Meroblastic
Formation of three-layered organism in its proper orientation
Gastrulation
Five examples of gastrulation movements
Invagination, involution, ingression, delamination, epiboly,
Three derivatives of somites
Myotome, sclerotome, dermatome
Germ layer origin of notochord
Mesoderm
Germ layer origin of somites
Mesoderm
Germ layer origin of embryonic gut
Endoderm
Germ layer origin of epidermis
Ectoderm
Germ layer origin of brain
Ectoderm
Germ layer origin of liver
Endoderm
The formation of the ______ characterizes gastrulation in Dm
Ventral furrow
Reason why hen only has one functional ovary
Need ample space for egg to develop
Can a hen lay more than one egg per day?
Yes
Where does the sperm enter the chick egg to fertilize it?
Anywhere
Meaning of macrolecithal
Large yolk
Outermost layer of egg shell
Cuticular layer
Only egg shell layer that is not made of calcium carbonate
Cuticular layer
Middle layer of egg shell
Spongy layer
Composition of spongy and mammillary layers
Calcium carbonate
End of the egg with most pores
Blunt end
Which of the two (inner/outer cell membrane) is made of thin elastin-like fibers of uniform thickness?
Inner layer
Which of the two (inner/outer cell membrane) is made of thick elastin-like fibers of non-uniform thickness?
Outer layer
Antibacterials in albumen
Lysozyme, ovotransferrin, avidin
Antivirals in albumen
Ovomucin, cystatin
Function of avidin
Binds to biotin
Disease caused by eating too many raw egg
Egg white disease
Living component of egg that contains viable embryo
Germinal disc
Difference between blastodisc and blastoderm
Blastoderm - fertile; blastodisc - infertile
Blastoderm indicates ____ egg?
Fertile
Blastodisc indicates ____ egg?
Infertile
What stage of development is a chick egg in once it is laid?
Gastrulation
Description of chick cleavage
Meroblastic discoidal
Cells produced during cleavage
Blastomeres
Two layers formed at the end of cleavage
Epiblast, Hypoblast
Layer of blastoderm that makes embryo
Epiblast
Layer of blastoderm that makes extraembryonic structures
Hypoblast
Space in center of blastoderm that separates cells from yolk
Subgerminal cavity
Function of subgerminal cavity
Separates cells from yolk
Characterizes chick gastrulation
Hensen’s node @ primitive streak
Type of fertilization in mouse
Internal
Stage of meiosis at which the mouse egg cell is in, once it gets fertilized?
Meiosis II
Does physiological polyspermy occur in mice?
No
The two layers of blastocyst formed during mouse cleavage
Inner cell mass, trophectoderm
Fate of trophectoderm
Extraembryonic structures
Fate of inner cell mass
Mouse embryo
Why more practical to use small model systems?
Cheaper, faster, less complicated
Characteristics of good model organisms (4)
1) easy to manipulate
2) good genetics
3) easy to propagate
4) cheap and abundant
Why is Dm a good model organism (4)
Amenable to mutagenesis; Ubiquitous and abundant; Well-known genetics and development; Easy to maintain in the laboratory
Why is Zf a good model organism (3)
Produces many offspring
External fertilization
Amenable to complex manipulations
Why is Gallus domesticus a good model org (3)
Highly available
Embryos are similar to those of mammals
Staging is simple
Why not Gallus domesticus? (2)
Hard to study embryos within eggs
Poor genetics
Why mus musculus (3)?
Have many genes homologous to humans during development
Similar to human embryos
good genetics
Why not? (1)
Difficult to follow development
Eight gastrulation movements
invagination, involution, delamination, ingression, epiboly, migration, intercalation, convergent extension
When does eclosion occur (What day)?
Day 11 or 12
3 components of the culture medium in Dm
1) potato/corn meal
2) propionic acid
3) yeast
Type of cleavage of Dm?
Meroblastic superficial
During the larval period, the larva grows and molts _______, separating the period into three instars
twice
Gestation period of chick
19-21 days
Why do hens have only one fxnal ovary?
Because having two mature ovaries will not give enough space for the egg to develop and traverse the oviduct.
How do double egg yolks occur?
When two ovarian follicles develop at the same rate, they will be simultaneously ovulated
Why is albumen yellowish in color?
Contains riboflavin or ovoflavin (carotenoids)
How long is sperm viable in chick?
2-3 weeks
Precursor of Hensen’s node
Koller’s sickle region
After how long will all the primordial organs have formed?
30 hours
Fate of optic vesicle
optic cupp
fate of infundibulum
pituitary gland
fate of epiphysis
pineal gland
is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut
Kuppfer’s vesicle