Drosophila Flashcards
Insect with nymph stages before adult stage
Hemimetabolous insect
Storage for sperm within the female Dm’s body
Seminal receptacle and paired spermathecae
Characteristics of Dm eggs
One half mm in lengthWhiteOval shapedSlightly flattened
Inner thin membrane surrounding the ovum
Vitelline envelope
Outer, tough extracellular coat surrounding the ovum
Chorion
Surface of Dm from which the respiratory filaments emerge
Dorsal surface
Extensions of the chorion found at the anterior end of the Dm egg, functions in gas exchange
Respiratory filaments
Optimal temperature for Dm egg hatching
25 degC
Largest of all larval forms
Third instar larva
Name for adult fly
Imago
Undifferentiated, mitotic cells within the Dm larva
Imaginal discs and histoblasts
Cells that will form epidermal structures of the adult Dm
Imaginal discs
Stage wherein imaginal discs evert and differentiate into adult structures
Pupal stage
Cells in larva that form the abdominal epidermis and internal organs of adult
Histoblasts
Recognized within larva as clumps of small cells
Histoblasts
Teardrop shaped epithelial cells in larva
Imaginal discs
Process of emerging as an adult from the pupa or as a larva from the egg
Eclosion
Time of day wherein females produce the greatest number of eggs
Late afternoon, evening
Differences between male and female Dm
Males have black pigmentation on the posterior abdominal segment; shorter abdomen (six segments), have a sex combFemales don’t have black pigmentation; have eight abdominal segments; don’t have a sex comb
Bristles found on the end of the first segment of the front legs of male Dm
Sex comb
What comprises an acceptance signal from a female Dm during mating?
Slowing down, extrusion of ovipositor, spreading of wings
What comprises a rejection from a female Dm during mating?
Kicking with hind legs, fending with middle legs, flicking of wings, buzzing sound, rapid movement, extrusion of genitalia
Organs through which female Dms perceive the love song
Antennae
Process by which male Dms generate their love song
Vibration of one wing
Feathery extensions of antennae that augment vibrations
Aristae
Segment of antennae that senses vibrations during mating
Johnston’s organ
Segment wherein Johnston’s organ is found
Second segment of antennae
Organ male Dms extend to touch female Dm genitalia
Proboscis
Process by which egg emerges from female Dm ovipositor
Oviposition
Way eggs emerge from the female Dm ovipositor
Posterior end first
Mold inhibitor used in culturing Dm
Propionic acid
Function of propionic acid
Mold inhibitor
Balanced salt solution which allows continued development of egg until hatching
Ringer’s solution
What composes insect Ringer’s solution?
NaCl, KCl, CaCl2
Extensions of the chorion
Respiratory filaments and micropyle
Channel through which sperm travels towards the ovum
Micropyle
Substitute for insect Ringer’s solution
PBS
Dye used to visualize Dm ornamental markings
Toluidine blue
Leaves impressions on the chorion prior to ovulation
Ovarian follicle cells
Impressions left by ovarian follicle cells on Dm egg chorion
Ornamental markings
Advantages of respiratory filaments as respiratory organs of Dm
Minimal water loss, maximized gas exchange
A film of gas surrounding the respiratory filaments when submerged
Plastron
Allows respiratory filaments to function even when submerged
Plastron
Maintains plastron
Water-repellent surface network of respiratory filaments
Type of egg wherein yolk is concentrated centrally and cytoplasm is pushed to the periphery
Centrolecithal egg
Nuclear division
Karyokinesis
Cleavage of cytoplasm
Cytokinesis
Usual process of cleavage (not like the formation of a multinucleate cell in early Drosophila development)
Karyokinesis then cytokinesis
Stage of Dm embryo wherein many nuclei are lined up in the peripheral cytoplasm
Syncytial blastoderm
Multinucleate tissue in which cell membranes don’t completely separate the nuclei
Syncytium
Stage of Dm embryo wherein cell membranes grow inward between peripheral nuclei, separating off individual cells from inner yolky cytoplasm
Cellular blastoderm
Pattern of cleavage in Dm embryo
Superficial or peripheral cleavage
First cells that form during cellularization
Pole cells
End of embryo where pole cells form
Posterior end
Origin of primordial germ cells
Pole cells
Fate of pole cells
Primordial germ cells
Fate of primordial germ cells
Gametes
Process following cleavage
Gastrulation
Insect with larval and pupal stage prior to adult stage
Holometabolous insect
Larval organs that are preserved
Malpighian tubules, fat bodies, gonads, nervous system
What regions comprise the head? (3)
mandibular, maxillary, labial
How many thoracic segments?
3
How many abdominal segments?
8
Segments that form the telson
Abdominal segments 9 and 10
Brings food to the mouth
mouth hooks
Does the larva have eyes?
No
hardened outer covering of flies, made of chitin with protein called sclerotin
cuticle
What is distributed over the cuticle?
sensory bristles
long white sheets running the length of the body
Fat bodies
Lateral to the esophagus
salivary glands
Sequence of compartments of GI tract
Pharynx –> Esophagus –> Proventriculus –> Gastric area –> midgut –> hindgut
Proventriculus empties into what?
gastric area
Excretory organs that carry urinary waste from the body
Malpighian tubules
Type of circulatory system?
Open
Bathes the internal organs
Hemolymph
Nine pairs of discs + 1 disc
labial, clypeolabrum, humerus, antenna, wing, haltere, 3 pairs of leg discs. genital disc
Chromosomes that replicate without separating
polytene chromosomes
What layer transforms into the puparium?
cuticle
Marks the end of pupation and beginning of adult life
Eclosion
White puparium
P1
Brown puparium
P2
Bubble prepupa
P3
Buoyant/moving bubble
P4
Malpighian tubules white
P5
Green Malpighian tubules
P6
Yellow body
P7
Yellow eyed
P8
Amber eyed
P9
Red eyed
P10
Head and thoracic bristles
P11
Wings grey
P12
Wings black
P13
Green patch
Meconium
Waste products of pupal metabolism
Meconium
Eclosion
P15
Appearance of green patch
P14
Layer of cells formed in embryo during cleavage
blastoderm
A furrow in the head region
cephalic furrow
Principal component of cuticle
chitin
Removal of the chorion
Dechorionation
Row of small hooks that help larva move by gripping substrate
denticle belt
Another word for molting
ecdysis
Simple eye containing a single light-perceivng element covered by a lens
Ocellus
Light-sensitive elements of the compound eye
ommatidium
Where larva exits
operculum
What stages are considered to be prepupa?
P1-P4
What stages are considered to be pupal?
P5-P15
Mouth parts of adult fly
proboscis
helps male pull himself up onto the female’s back during mating
sex combs
Color of the filter you will use to increase contrast of orange?
Blue
Germ layer that gives rise to epidermis/ectoderm
ectoderm
3 main functions of the condenser
control concentrate collect
The process of turning inside out
eversion
Footprints of ovarian follicle cells on the embryo
ornamental markings
number of eggs a femal Dm lays in one day
50-70
Prevents growth of molds/other bacteria
propionic acid
fertilization with more the one sperm cell
polyspermy
Process of turning inside out
Eversion
Embryological tool for fin manipulation and dissection of embryos
microneedle
Number of spiracles in a first instar larva
2
Considered the Mercedes of all lenses
Planapochromat
Rotate polarized lightd
birefringence
sac on the fly head which becomes filled with blood to break open the operculum
ptilinium
term used to describe Dm embroy before cleavage
syncytial blastoderm
second indication that larva enters pupation
stops moving
Process of egg laying in Dm
oviposition