Purine Synthesis (Nucleotide Synthesis)

Question 1

what is the parent molecule of Purine synthesis?

Answer 1

IMP (Inosine Monophosphate)

Question 2

sources of the Nitrogen atoms in Purine synthesis

Answer 2

Aspartate, Glutamine, Glycine

Question 3

sources of the Carbon atoms in Purine synthesis

Answer 3

N5-N10-Formyl-THF (tetra hydra folate), Glycine, Respiratory CO2

Question 4

name the ring (free nitrogenous base) that is a precursor to the Purine molecule.

Answer 4

Hypoxanthine

Question 5

what is needed to convert Ribose-5-P –> PRPP (phosphoribosyl pyrophosphate)?

Answer 5

4ATP + Mg

Question 6

what AA + high energy compound converts IMP –> AMP?

Answer 6

Aspartate + GTP

Question 7

what AA + high energy compound converts IMP –> GMP?

Answer 7

Glutamine + ATP

Question 8

In the Salvage pathway for Purine synthesis

what is the rxn + enzyme to make AMP?

Answer 8

Adenine + PRPP –> AMP
enzyme: APRT

Adenine Phosphoribosyl Transferase

Question 9

In the Salvage pathway for Purine synthesis

what are the 2 rxns + enzyme to make IMP/GMP

Answer 9

Hypoxanthine + PRPP –> IMP
Guanine + PRPP –> GMP
enzyme: HGPRT

Hypoxanthine-guanine Phosphoribosyl Transferase

Question 10

In the Salvage pathway for Purine synthesis

which tissues are unable to use the Salvage pathway for Purine Synthesis?

Answer 10

Brain, RBC, WBC

Question 11

Where does Salvage nucleotide synthesis occur

Answer 11

Liver