Purification + Characterization Of Proteins

Question 1

Name the methods that separate proteins by SIZE

Answer 1

Filtration + Dialysis
(Gel filtration, dialysis + ultrafiltration)

Question 2

Name the methods that separate proteins by CHARGE

Answer 2

Chromatography (Chr = charge)
Ion-exchanging chromatography, affinity chromatography, HPLC (high performance liquid chromatography)

Question 3

Name the method that separates proteins by PURITY + MOLECULAR WEIGHT

Answer 3

SDS-PAGE (sodium dodecyl sulfate polyacrylamide electrophoresis)

Question 4

Name the method that determines the PRIMARY SEQUENCE OF PROTEIN

Answer 4

Edman degradation