Pulm/Renal - Biochemistry - Amino Acid Metabolism: Inborn Errors of Metabolism, Porphyrias, Jaundice Flashcards
Amino acids whose citric acid cycle intermediates can be subsequently converted to ____________ are termed glucogenic as they are easily entered into gluconeogenesis.
Amino acids whose citric acid cycle intermediates can be subsequently converted to ____________ are termed ketogenic as they are easily entered into fatty acid or ketone synthesis.
Amino acids whose citric acid cycle intermediates can be subsequently converted to ____________ are termed both glucogenic and ketogenic as they are easily entered into both of the above pathways.
Oxaloacetate;
acetyl-CoA;
pyruvate
Amino acids whose citric acid cycle intermediates can be subsequently converted to pyruvate are termed ___________.
Amino acids whose citric acid cycle intermediates can be subsequently converted to oxaloacetate are termed ___________.
Amino acids whose citric acid cycle intermediates can be subsequently converted to acetyl-CoA are termed ___________.
Both ketogenic and glucogenic;
glucogenic;
ketogenic
Which are the amino acids that are solely ketogenic (2)?
What does this mean?
Leucine, lysine;
they are easily converted to acetyl-CoA
Which are the amino acids that are solely glucogenic (9)?
What does this mean?
Arginine
Asparagine & Glutamine
Aspartate & Glutamate
Histidine
Methionine
Proline
Valine;
they are easily converted to oxaloacetate
Which are the amino acids that are either ketogenic or glucogenic (9)?
What does this mean?
Alanine
Cysteine
Glycine
Isoleucine
Phenylalanine
Serine
Threonine
Tryptophan
Tyrosine;
they are readily converted to pyruvate (which is easily convertible to acetyl-CoA and oxaloacetate)
Decide (for each AA individually) if each of the following amino acids is ketogenic, glucogenic, or both:
Proline
Tryptophan
Serine
Valine
Methionine
Glucogenic
Both
Both
Glucogenic
Glucogenic
Decide (for each AA individually) if each of the following amino acids is ketogenic, glucogenic, or both:
Threonine
Histidine
Lysine
Isoleucine
Glutamine
Both
Glucogenic
Ketogenic
Both
Glucogenic
Decide (for each AA individually) if each of the following amino acids is ketogenic, glucogenic, or both:
Glutamate
Aspartate
Phenylalanine
Asparagine
Aspartate
Glucogenic
Glucogenic
Both
Glucogenic
Glucogenic
Decide (for each AA individually) if each of the following amino acids is ketogenic, glucogenic, or both:
Arginine
Cysteine
Leucine
Tyrosine
Glycine
Glucogenic
Both
Ketogenic
Both
Both
Any amino acid that can be converted to (1) _________ is termed both glucogenic and ketogenic because (1) can be converted both to (2) _________ and (3) _________.
(Note: not all AA that are both glucogenic and ketogenic can be converted to (1). Some can enter the citric acid cycle at multiple intermediates.)
(1) Pyruvate,
(2) acetyl-CoA, (3) oxaloacetate
What are the (1) buildup product(s) and (2) deficient enzyme of homocystinuria?
Homocysteine (and possibly methionine);
cystathione β-synthase
What are the (1) buildup product(s) and (2) deficient enzyme of maple syrup urine disease?
Branched-chain ketoaciduria;
branched-chain α-ketoacid dehydrogenase complex
What are the (1) buildup product(s) and (2) deficient enzyme of methylmalonic acidemia?
L-Methylmalonic acid (and possibly propionyl-CoA);
methylmalonyl-CoA mutase
What are the (1) buildup product(s) and (2) deficient enzyme of phenylketonuria?
Phenylalanine;
phenylalanine hydroxylase
What are the (1) buildup product(s) and (2) deficient enzyme of alkaptonuria?
Homogentisate;
homogentisate oxidase
What are the (1) buildup product(s) and (2) deficient enzyme of type I tyrosinemia?
Fumarylacetoacetate;
fumarylacetoacetate hydrolase
What are the (1) buildup product(s) and (2) deficient enzyme of type II tyrosinemia?
Tyrosine;
tyrosine aminotransferase
What inborn error of metabolism is associated with a buildup of homocysteine (and possibly methionine)?
What enzyme is deficient?
Homocystinuria;
cystathione β-synthase
What inborn error of metabolism is associated with a buildup of branched-chain α-ketoacids?
What enzyme is deficient?
Maple syrup urine disease;
branched-chain α-ketoacid dehydrogenase complex
What inborn error of metabolism is associated with a buildup of L-methylmalonic acid?
What enzyme is deficient?
Methylmalonic acidemia;
methylmalonyl-CoA mutase
What inborn error of metabolism is associated with a buildup of phenylalanine?
What enzyme is deficient?
Phenylketonuria;
phenylalanine hydroxylase
What inborn error of metabolism is associated with a buildup of homogentisate?
What enzyme is deficient?
Alkaptonuria;
homogentisate oxidase
What inborn error of metabolism is associated with a buildup of fumarylacetoacetate?
What enzyme is deficient?
Type I tyrosinemia;
fumarylacetoacetate hydrolase
What inborn error of metabolism is associated with a buildup of tyrosine?
What enzyme is deficient?
Type II tyrosinemia;
tyrosine aminotransferase
Phenylketonuria is basically an inability to convert ____________ to ___________.
Why does this affect neurotransmitter production?
Phenylalanine, tyrosine;
tyrosine is a precursor for catecholamine synthesis
Asides from a phenylalanine hydroxylase deficiency (the most common cause), what other issues might cause phenylketonuria?
(1) A deficiency of tetrahydrobiopterin (BH4) (a cofactor for phenylalanine hydroxylase and catecholamine synthesis);
(2) an inability to regenerate BH4 from BH2 (via dihydropteridine reductase)
A 45-year-old male presents with complaints of worsening arthralgias. Upon further studies, you note that his urine turns brown when left exposed to air.
What is at the top of your differential?
What deficiency is responsible?
Alkaptonuria;
the enzyme homogentisate oxidase
How is maple syrup urine disease managed?
Via restriction of branched-chain amino acids
(valine, isoleucine, leucine)
What substrate may build up in cases of severe B12 deficiency, causing acidemia?
This is because a certain enzyme involving acids requires B12 as a cofactor. Which is it?
This is an alternate cause of what type of acidemia?
L-Methylmalonic acid;
methylmalonyl-CoA mutase;
methylmalonic acidemia
What are the two major methyl donors in the body?
S-Adenosylmethionine;
folate (and its derivatives)
What two vitamins are needed for S-adenosylmethionine (SAM) (a methyl donor) regeneration?
After donating its methyl group, SAM becomes S-adenosylhomocysteine, then homocysteine, then methionine.
In which part of this chain are the two vitamins necessary?
B12 and folate;
the conversion of homocysteine to methionine
After donating its methyl group, S-adenosylmethionine (SAM) becomes S-adenosylhomocysteine, then homocysteine.
What are the two products that are typically now produced from homocysteine?
Which vitamins are required for each path?
Either: (1) methionine (to regenerate SAM), B12 and folate;
or (2) cystathione, B6 (pyridoxal phosphate)
Methylmalonyl-CoA can be created from the metabolism of propionyl-CoA. It is then often converted to _____________ to enter the citric acid cycle.
What enzyme and vitamin are involved in this conversion?
Succinyl-CoA;
methylmalonyl-CoA mutase, B12
A deficiency of which of the following can cause an elevation in blood homocysteine: vitamin B12, vitamin B6, or folate?
A deficiency of which of the following can cause an elevation in blood L-methylmalonic acid: vitamin B12, vitamin B6, or folate?
All three;
B12 only
What acid often builds up in addition to L-methylmalonic acid?
Propionic acid
If poorly controlled, which of the following inborn errors of metabolism are most likely to cause intellectual disability and other neurological disabilties?
Alkaptonuria
Type I tyrosinemia
Type II tyrosinemia
Phenylketonuria
Homocystinuria
Maple syrup urine disease
Methylmalonic acidemia
Type II tyrosinemia
Phenylketonuria
Homocystinuria
Maple syrup urine disease
Methylmalonic acidemia
How does type I tyrosinemia often present?
A cabbage-like odor in the urine;
liver failure, renal tubular acidosis
Which are the *conditionally essential amino acids (6)?
(*E.g. essential during growth, pregnancy, illness, etc.)
Arginine
Cysteine
Glutamine
Glycine
Proline
Tyrosine
Which are the nonessential amino acids (5)?
Alanine
Asparagine
Aspartate
Glutamate
Serine
State if the following amino acid is essential, conditionally essential, or nonessential.
Second, state if the following amino acid is glucogenic, ketogenic, or both.
Third, name the citric acid cycle intermediate(s) into which it is readily convertible.
Alanine
Nonessential;
both;
pyruvate
First, state if the following amino acid is essential, conditionally essential, or nonessential.
Second, state if the following amino acid is glucogenic, ketogenic, or both.
Third, name the citric acid cycle intermediate(s) into which it is readily convertible.
Arginine
Conditionally essential;
glucogenic;
α-ketoglutarate
First, state if the following amino acid is essential, conditionally essential, or nonessential.
Second, state if the following amino acid is glucogenic, ketogenic, or both.
Third, name the citric acid cycle intermediate(s) into which it is readily convertible.
Asparagine
Nonessential;
glucogenic;
oxaloacetate