PT - Modelling Membrane Protein Structure

Question 1

What are the 3 main problems with membrane protein structure determination?

Answer 1

1. Protein expression
2. Solubilization/Purification//Reconstitution
3. Structural Analysis

Question 2

What is the hydropathy plot?

Answer 2

Hydropathy plots allow for the visualization of hydrophobicity over the length of a peptide sequence

Question 3

What is Genomics Analysis?

Answer 3

Genomic analysis is the identification, measurement or comparison of genomic features such as DNA sequence, structural variation, gene expression, or regulatory and functional element annotation at a genomic scale

Question 4

What are the 2 main helices that are present in bilayers?

Answer 4

Alpha Helix (Main)
Beta Barrel Structure (Minor)

Question 5

What is a sucrose specific porin?

Answer 5

A Transmembrane protein that allows for the uptake of sucrose under growth-limiting conditions

Question 6

How can the location of glycoslylation be determined?

Answer 6

Markers on threonine and serine indicate where glycosylation occurs and hence show where the outside of the cell is