Pseudomonas and Spirochetes

Question 1

Pseudomonas aeruginosa is gram _____ and shape of?

Answer 1

Gram negative rod shaped bacterium

Question 2

What type of pathogen is Pseudomonas aeruginosa

Answer 2

opportunistic

Question 3

Pseudomonas aeruginosa is commonly isolated from where?

Answer 3

soil and water

Question 4

Pseudomonas aeruginosa is associated with

Answer 4

• Respiratory infectios such as cystic fibrosis lung infections
• wound
• burns
• diabetic foot infections
• UTIs
• Septicemia
• Nutritionally versatile

Question 5

Pseudomonas aeruginosa grows how?

Answer 5

In biofilms often in polymicrobial communities

Question 6

Pseudomonas aeruginosa is controlled how?

Answer 6

controlled by quorum sensing (QS)

Question 7

Polymicrobial communities

are composd of

Answer 7

baceria, fungi, viruses

Question 8

Polymicrobial communities

found where?

Answer 8

natural environments, normal flora and infection sites

Question 9

Polymicrobial infections can do what to disease?

Answer 9

exacerbate disease

Question 10

Cystic Fibrosis is what?

Answer 10

Hereditary disease affecting the mucus membranes of lungs and other organs

Question 11

what is the result of Hereditary disease affecting the mucus membranes of lungs and other organs

Answer 11

Results in accumulation of copious amounts of mucus (sputum)

Question 12

Cystic Fibrosis causes what?

Answer 12

Chronic lung infections with gram negative and gram positive bacteria

Question 13

P. aeruginosa can be isolated from _____% of individuals with CF

Answer 13

over 90%

Question 14

P. aeruginosa progression in the CF lung

Answer 14

from birth to age 8 contains H. influenza, A. aureus, and P. aeruginosa. However afte age 8 (8-37) contains merely P. aeruginosa

Question 15

P. aeruginosa reach counts as high as ______ in CF sputum

Answer 15

10^9 CFU/ml

Question 16

P. aeruginosa is the primary cause of what in cystic fibrosis patients?

Answer 16

morbidity and mortality

Question 17

Why does P. aeruginosa grow so well in the CF lung?

Answer 17

CF contains amino acids, sugars, lipids and ions. P. aeruginosa can catabolize many of these carbon substrates. CF sputum is a growth substrate for this organism

Question 18

P. aeruginosa growth in vivo has a correlation between what?

Answer 18

virulence factors produced and available carbon source

Question 19

what is the Quorum sensing in P. aeruginosa?

Answer 19

Free diffusible: 
    - lasl/R (3-oxoC12HSL)
    -rhll/R (C4HSL)
Delivered in vesicles form: 
    -pqsA-E, pqsR PQS