Listeria Flashcards
Listeria monocytogenes is found where at what temperature?
Reservoir in soil and sometimes found in animals. It can grow at 4 degrees Celsius.
Listeria monocytogenes is a gram _____ bacteria with a shape _____
gram positive bacteria with coccobacilli
Listeria monocytogenes caused food born infections from what in California in 2011 resulting in 30 deaths?
Cantaloupes
What is the symptoms of Listeria monoctogenes?
- Fever
- Muscle aches (resembling flu)
- sometimes diarrhea
Is Listeria monocytogens different in immunocompromised people?
Yes it is worse in those immunocompromised
What extra risk do pregant woman with L. monocytogens have?
- risk of miscarriage
- stillbirth
- premature delivery
- neonatal infections (high mortality)
What is the pathway of Listeria monocytogens?
Goes from contaminated food —–> Intestines —-> to the blood stream which —-> goes to both the liver and the spleen —-> continues through the blood stream and goes to both —-> brain and placenta.
Listeria virulences include what:
- M cells
- Internalin
- Listeriolysin O (llo)
- PlcA and Plc B
- Listeriolysin S
- Actin polymerization gene (act A )
How does M cells play a role in Listeria’s virulence?
Enters through M cells in the intestinal mucosa.
How does Internalin (inlA, inlB) play a role in Listeria’s virulence?
entry into cells
How does Listeriolysin O (llo) play a role in Listeria’s virulence?
it is a pore formin cyotoxin that promotes the escape from vacuole; required for escape in mouse
How does PlcA and PlcB play a role in Listeria’s virulence?
pore forming phospholipases required for escape in humans
Listeriolysin S is a virulence factor of Listeria which is similar to what?
Streptolysin S
How does Actin polymerization gene (actA) play a role in Listeria’s virulence?
intracellular transport
How does the Actin polymerization gene promote intracellular transport?
The surface protein ActA mimics the host proteins, recruits the host Arp2/3 complex, and promotes actin polymerization, formation of an actin tail, and a movement from cell to another cell.
To avoid Listeria pregnant woman should avoid eating what?
soft cheese
What happens to listeria at room temperature
causes hemolysis of RBC at room temperature (CAMP factor)
The bacteriophage (Listeria phage P100) is what?
approved by FDA in 2006 to spray on food
The Anton test detects what?
infection of Listeria in rabbit eye.
What is used to treat listeria?
Amoxicillin and gentamicin.
What is Listeria resistant to?
Tetracycline and trimethoprim
