proteins - 1 Flashcards
2 thing proteins are made of
amino acids
one or more polypeptides
whats a dipeptide
two amino acids joined together
whats a polypeptide
more than two amino acids join together
amino acid structure
carboxyl group (-COOH)
anime group
R group attached to carbon atom
all living things contain how many amino acids?
20
what reaction links amino acids
condensation
bonds formed between amino acids
peptide bonds
what happens when dipeptides and polypeptides are broken down
reverse reaction - hydrolysis
protein structure (4)
primary, secondary, tertiary, quaternary
primary structure
sequence of amino acids in polypeptide chain
secondary structure
hydrogen bonds form between amino acids in chain
automatically coils into alpha helix or fold into beta pleated sheet
tertiary structure
disulfide bridges form when two amino acids of cysteine come close together
more bonds form
become more coiled or folded
TIP- think of this as a big tangled spring
quaternary structure
polypeptide chains are assembled together
proteins final 3D structure
test for protein
biuret test
3 steps for biuret etst
- test needs to be alkaline - add a few drops of sodium hydroxide
- add copper sulfate solution
- if protein is present, solution turns purple