all cells arise from other cells - 2 Flashcards

topic 2

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1
Q

4 stages of cell cycle

A

mitosis, gap phase 1, synthesis, gap phase 2

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2
Q

structure of chromosomes

A

2 strands called sister chromatids joined in the middle by a centromere

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3
Q

4 stages of mitosis in order

A

prophase
metaphase
anaphase
telophase

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4
Q

what happens in prophase

A

chromosomes condense and get shorter and fatter
centrioles start moving to opposite ends to cell
nuclear envelope breaks down

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5
Q

what’s happens in metaphase

A

chromosomes line up along the middle of the cell and become attached to the spindle by their centromere

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6
Q

what happens in anaphase

A

centromere divides
spindles contract pulling the chromatids to opposite poles

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7
Q

what happens in telophase

A

chromatids uncoil and become long and thin again turning into chromosomes
nuclear envelope forms
cytoplasm divides, cytokinesis, and two daughter cells are formed

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8
Q

what is cancer

A

uncontrolled cell division

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9
Q

what do cancer treatments aim to do

A

control the rate of cell division in tumour cells by distrusting the cell cycle

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10
Q

what practical is used in this side topic

A

root tip squash

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11
Q

preparing a root tip cell squash (8)

A
  1. 1M hydrochloric acid to a boiling tube in a water bath at 60degrees
  2. use a scalpel to cut 1cm from the tip of a root
  3. transfer root tip to boiling tube and incubate for 5 mins
  4. use a pipet to rinse well with cold water and dry the tip
  5. place on a microscope slide and cut 2mm from the end of the tip
  6. use a mounted needle to spread out cells thinly
  7. add a few drops of stain
  8. place a cover slip and press down
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12
Q

how to calculate the mitotic index

A

number of cells with visible chromosomes/ total number of cells observed

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13
Q

calculating the actual size of cells

A

size of image / magnification

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