Protein synthesis Flashcards

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1
Q

cell organelles involved in protein synthesis

A
  • Nucleus
  • Ribosome
  • Endoplasmic reticulum
  • Golgi body
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2
Q

what is a codon

A

group of three nucleotides (every three codes for a specific amino acid)

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3
Q

gene

A

group of codons (300-500) - codes for a specific protein

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4
Q

start codon

A

methionine

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5
Q

how many codons are there

A

64

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6
Q

how many codons code for amino acids

A

61

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7
Q

how many stop codons are there

A

3

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8
Q

what is the central dogma

A

the prosses of protein synthesis
- turning DNA to RNA and finally into a polypeptide

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9
Q

transcription (elongation)

A
  • builds an mRNA strand that is complementary to the transcribed DNA strand with the substitution of uracil in place of thymine
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10
Q

transcription (initiation)

A
  • RNA polymerase binds to the promoter segment of DNA
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11
Q

promoter region

A
  • high in adenine and thymine
  • a recognition site for RNA polymerase (TATA box)
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12
Q

template strand

A

strand of DNA the RNA polymerase is transcribing

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13
Q

coding strand

A

strand of DNA that is left alone during transcription

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14
Q

Transcription (termination)

A
  • RNA polymerase releases once it hits the terminator sequence
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15
Q

after transcription

A

the hydrogen bonds reform once again connecting the DNA strands

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16
Q

post transcription modification

A

caping and tailing

17
Q

caping

A

5’ cap is added to the start of mRNA

18
Q

tailing

A

a string of 200 adenine ribonucleotides is added to the end 3’ with assistance from poly-A- polymerase giving it the name Poly- A- tail

19
Q

Introns and Extrons

A
  • introns are “useless” DNA and exons are important for protein synthesis/ folding
  • introns are removed from mRNA after transcription, but before it leaves the nucleus
  • if the introns are left in they will alter the folding of the protein rendering it usless
20
Q

how are introns removed

A
  • spliceosomes (remove introns and connect exons)
21
Q

what happens to introns once they are removed

A

their nucleotides are broken down and recycled

22
Q

ribosome structure

A

2 units, built independently and then joined together
- large subunit (60S)
- small subunit (40S)
binding sites A (acceptor cite) and P (peptide cite)
- each site can hold one codon at a time

23
Q

purpose of the ribosome

A

translate mRNA and build the polypeptide chain

24
Q

tRNA

A
  • transfer RNA
  • single-stranded nucleic acid (5’- 3’)
  • cross-shaped
  • delivers the indicated amino acids
  • binds to ONE specific amino acid
25
Q

anticodon

A

located on one of tRNA’s arms
- complementary to mRNA codons being read by the ribososme

26
Q

aminoacyl-tRNA-synthetases

A

attaches the appropriate amino acid to tRNA

27
Q

Translation (initiation)

A

ribosome recognizes and binds to the mRNA 5’cap

28
Q

translation (elongation)

A

mRNA moves through the ribosome 5’-3’
- the starter codon conects at the p site and the following codon connects to the the a cite

29
Q

what is the start codon

A

AUG

30
Q

what bond connects the amino acids

A

peptide bond

31
Q

termination step one

A

ribosome reaches the stop codon and the release factor will let go of the polypeptide chain

32
Q

termination step 2

A

polypeptide enters the Golgi apparatus to be folded and stored or sent out right away in a vesicle