DNA replication Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

who discovered that the structure of DNA made it easy to replicate?

A

Watson and crick
(proven in 1958 by Mathew Meselson and Flranklin Stahl)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

how does DNA replicate

A

semi-conservative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what type of strands will each daughter cell have?

A

one parent strand and one copy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how many replication orgins do prokaryotes have

A

One

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what must DNA do before a cell can divide

A

create a copy of its self during mitosis or meosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

when does DNA replicate

A

during the S phase of interphase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

how does DNA replicate in eukaryotes

A
  • multiple replication orgins
  • happens in both directions
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

replication bubble

A

when two replication forks are near eachother it forms a bubble

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

replication fork

A

where the two strands are still conected together while being seperated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what are to five DNA replication enzymes

A
  • DNA helicase
  • DNA gyrase
  • DNA polymerase III
  • DNA polymerase I
  • DNA ligase
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

DNA helicase

A

unzips/ unwinds the double helix by breaking the hydrogen bonds between the nitrogenous bases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

DNA Gyrase

A

relives the tension built up from DNA helicase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

SSBP

A

single-stranded binding proteins

added by and enzyme to bind the sugar phasphate backbone. to each strand, every ssbp is the same charge so they rapel away from eachother keeping the strands apart so that DNA can replicate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

DNA polymerase III

A

takes nucleotides and inserts them building the daughter strand, this causes the SSBPś to let go
- this enzyme will only move 5’ to 3’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Leading strand

A

strand of DNA that is orientated 5’to 3’
- built continuously

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

lagging strand

A

built discontinuously in fragments

17
Q

RNA primers

A

create false starting sites on the lagging strand so polymerase III can move 5’ to 3’

18
Q

Okazaki fragments

A

the fragments of DNA built along the lagging strand

19
Q

DNA polymerase I

A

runs along the lagging strand eliminating the primers while simultaneously filling in any gaps
- can not form the ester bonds between the phosphate group and sugar

20
Q

DNA Ligase

A

builds the ester bonds completing the replication of the lagging strand

21
Q

proofreaders

A

DNA polymerase I and III